FAQ: Why have the NEB Glycosidase enzymes changed reaction buffers? What are the new reaction buffers and can I still use an enzyme with its old buffer? Where can I find the composition of the old buffers?

To simplify workflows and digestions with two or more exoglycosidases, most enzymes are now provided with 10X GlycoBuffer 1. Two exceptions are provided with GlycoBuffer 4. Also, the buffer panel for endoglycosidases has been simplified.

The activity of every endo- and exoglycosidase enzyme was evaluated in both its old and new buffer system. All enzymes retain either the same activity, or were found to have greater activity in the new buffer. All exoglycosidases now retain optimal activity in GlycoBuffer 1. As before, some exoglycosidases are still provided with an additional vial of BSA (BSA enhances the activity of certain enzymes). Other components (i.e. Glycoprotein Denaturing Buffer, NP-40, etc.) are still provided when required.

Enzyme Product # Old buffer Current buffer Changed?
α-N-Acetylgalactosaminidase P0734 G7 GlycoBuffer 1 YES
α1-2 Fucosidase P0724 G4 GlycoBuffer 1 YES
α1-2,3,4,6 Fucosidase P0748 --- GlycoBuffer 1 ---
α1-2,4,6 Fucosidase O p0749 --- GlycoBuffer 1 ---
α1-3,4 Fucosidase p0769 --- GlycoBuffer 1 ---
α1-2,3 Mannosidase P0729 G6 GlycoBuffer 1 no
α1-6 Mannosidase P0727 G2 GlycoBuffer 1 YES
α1-2,3,6 Mannosidase p0768 --- GlycoBuffer 4 ---
α1-3,4,6 Galactosidase P0747 --- GlycoBuffer 1 ----
α1-3,6 Galactosidase P0731 G6 GlycoBuffer 1 no
α2-3 Neuraminidase S P0743 --- GlycoBuffer 1 ---
α2-3,6,8 Neuraminidase P0720 G1 GlycoBuffer 1 YES
α2-3,6,8,9 Neuraminidase A P0722 --- GlycoBuffer 1 ---
β-N-Acetylhexosaminidasef P0721 G2 GlycoBuffer 1 YES
β-N-Acetylglucosaminidase P0732 G1 GlycoBuffer 1 YES
β-N-Acetylglucosaminidase S P0744 --- GlycoBuffer 1 ---
β1-3 Galactosidase P0726 G6 GlycoBuffer 1 no
β1-4 Galactosidase S P0745 --- GlycoBuffer 1 ---
β1-3,4 Galactosidase p0746 --- GlycoBuffer 4 ---
N-Glycan Sequencing Kit E0577 --- GlycoBuffer 1 ---
Endo S P0741 G6 GlycoBuffer 1 YES
Endo H P0702 G5 GlycoBuffer 3 YES
Endo Hf P0703 G5 GlycoBuffer 3 YES
Endo F2 p0772 --- GlycoBuffer 4 ---
Endo F3 p0771 --- GlycoBuffer 4 ---
Endo D P0742 G7 GlycoBuffer 2 no
O-Glycosidase P0733 G7 GlycoBuffer 2 no
O-Glycosidase & Neuraminidase Bundle E0540 G7 GlycoBuffer 2 no
Remove-iT® PNGase F P0706 G7 GlycoBuffer 2 no
PNGase F P0704 G7 GlycoBuffer 2 no
PNGase F (Glycerol-free) P0705 G7 GlycoBuffer 2 no
PNGase F, Recombinant P0708 G7 GlycoBuffer 2 no
PNGase F (Glycerol-free), Recombinant P0709 G7 GlycoBuffer 2 no
PNGase A p0707 --- Glyco Buffer 3 ---
Protein Deglycosylation Mix II p6044 --- Deglycosylation Mix Buffer 1 and 2 ---
Rapid PNGase F P0710 --- Rapid PNGase F Buffer ----
Rapid™ PNGase F (non-reducing format) p0711 --- Rapid PNGase F (non-reducing format) Buffer