Endo F2 is a highly specific recombinant endoglycosidase which cleaves within the chitobiose core of asparagine-linked complex biantennary and high mannose oligosaccharides from glycoproteins and glycopeptides. Endo F2 cleaves biantennary glycans at a rate approximately 20 times greater than high mannose glycans. The activity of Endo F2 is identical on biantennary structures with and without core fucosylation. However, Endo F2 is not active on hybrid or tri- and tetra-antennary oligosaccharides.
- Endo F2 is tagged with a chitin binding domain (CBD) for easy removal from a reaction
- Recombinant enzyme with no detectable exooglycosidase or other endoglycosidase contaminating activities
- Glycerol -free for optimal performance in HPLC and mass spectrometry analysis
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability for up to 24 months
Product SourceCloned from Elizabethkingia miricola (formerly Flavobacterium meningosepticum) and expressed in E. coli (1).
The following reagents are supplied with this product:
Store at (°C) Concentration GlycoBuffer 4 -20 10X
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