This article traces molecular cloning’s history and progress, highlighting how improved enzymes, assembly methods, and DNA design tools have advanced cloning efficiency, precision and applications.
NEBaseChanger® Primer Design Toolチュートリアルビデオでは、NEBaseChangerを用いて部位特異的および複数部位への変異導入用のプライマーをデザインする方法を説明します。このツールは、NEB Q5®およびNEBuilder® HiFi DNA Assemblyアプリケーションに最適です。
Explore NEB Expressions, featuring a precision medicine case study from discovery to targeted therapy, cell-free dbDNA synthesis, enzymatic solutions for circular RNA enrichment, conservation efforts in Costa Rica, RNA workflow products and the 2026 Passion in Science Awards.
RNase III Protocol Modification Can Enable Safe dsRNA Removal from IVT Samples
Posted on 2026年7月20日
By
Iris Martinez, Illustrations by Tasha José
High-quality and pure RNA is a requirement for RNA therapeutics to avoid unwanted immune responses, not just a “nice to have.” In vitro transcription can be optimized, but additional cleanup may be needed. Enzymatic removal of dsRNA using RNase III preserves ssRNA integrity when refined using a protocol developed by NEB scientists.
Cell-free Protein Synthesis Enables Rapid Single-chain Fragment Production and Analysis
Posted on 2026年7月9日
By
Iris Martinez, Illustrations by Tasha José
Cell-free protein synthesis (CFPS) workflows allow for fast, scalable and cost-effective single-chain antibody fragment production. NEB scientists have outlined approaches to quickly and efficiently screen the discovery and initial characterization of novel antibody fragments with CFPS.
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