PNGase F (Glycerol-free), Recombinant
Overview of Glycobiology
Peptide: N-Glycosidase F, also known as PNGase F, is a recombinant amidase which is supplied glycerol free for optimal performance in HPLC intensive methods. PNGase F cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins (1).
Product SourceCloned from Elizabethkingia miricola (formerly Flavobacterium meningosepticum) and expressed in E. coli (2).
The following reagents are supplied with this product:
Store at (°C) Concentration Glycoprotein Denaturing Buffer -20 10 X NP-40 10 % GlycoBuffer 2 4 10 X
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