β-N-Acetylhexosaminidasef is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing β-N-acetyl-galactosamine and glucosamine residues from oligosaccharides.
- Can be used for Epigenetic applications to remove O-GlcNAc from serine/threonine residues on transcription factors, histones, RNA-binding proteins and other O-GlcNAc modified proteins
- Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
- Glycerol -free for optimal performance in HPLC and mass spectrometry analysis
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability for up to 24 months
β-N-Acetylhexosaminidasef is a recombinant protein fusion of β-N-Acetylhexosaminidase (1) and maltose binding protein. It has identical activity to β-N-Acetylhexosaminidase. β-N-Acetylhexosaminidasef catalyzes the hydrolysis of terminal β-D-N-acetyl-galactosamine and glucosamine residues from oligosaccharides. β-N-Acetylhexosaminidasef can be used to remove O-GlcNAc (N-Acetylglucosaminidase on serine/threonine)(4).
- Epigenetics applications: O-GlcNac (N-Acetylhexosamine attached to serine or threonine) is a regulator of nuclear protein function. β-N-Acetylhexosaminidasef removes O-GlcNAc from transcription factors, histones, RNA-binding proteins, etc.
Product SourceCloned from Streptomyces plicatus
(1) and overexpressed in E. coli (2).
The following reagents are supplied with this product:
Store at (°C) Concentration GlycoBuffer 1 -20 10 X
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