Endo S
Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG
- Active on native IgG
- Tagged with a chitin binding domain (CBD) for easy removal
- Recombinant enzyme with no detectable exooglycosidase or other endoglycosidases contaminating activities
- Glycerol -free for optimal performance in HPLC and mass spectrometry analysis
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability for up to 12 months
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Product Information
Endo S is an endoglycosidase with a uniquely high specificity for removing N-linked glycans from the chitobiose core of the heavy chain of native IgG (1). Endo S is tagged with a chitin binding domain (CBD) for easy removal from a reaction and is supplied glycerol free for optimal performance in HPLC and MS intensive methods.Product Source
Endo S is cloned from Streptococcus pyogenes and overexpressed as a fusion to the chitin binding domain in E. coli.Specificity
Detailed Specificity
X = protein, peptide, Asparagine, or free glycan, as Endo S does not have a strict peptide requirement for activity. Endo S is active on a substrate with or without core α(1-6)fucosylation as well as a with or without a bisecting N-acetylglucosamine. Triantennary and tetrantennary sialyated or asialo glycans are not a substrate for Endo S (1).- This product is related to the following categories:
- Endoglycosidases Products,
- Proteome Analysis Products
- This product can be used in the following applications:
- Expression Systems,
- Glycan Sequencing,
- Proteomics,
- Recombinant Glycoprotein Expression, Glycoprotein Analysis
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