Home protein-tools α2-3,6,8 Neuraminidase

α2-3,6,8 Neuraminidase

α2-3,6,8 Neuraminidase catalyzes the hydrolysis of α2-3, α2-6, and α2-8 linked sialic acid residues from glycoproteins and oligosaccharides.

Recombinant enzyme with no detectable endoglycosidase or other exoglycosidases contaminating activities
Acts on both Neu5Ac and Neu5Gc
Fast digestion, removal of sialic acids in 5 minutes

Catalog #	Cat #	Concentration	Conc	Size
P0720S	50 000 units/ml	2 000 units	86,00 €	86,00 €
P0720L	50 000 units/ml	10 000 units	342,00 €	342,00 €
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Product Information
Neuraminidase is the common name for Acetyl-neuraminyl hydrolase (Sialidase). This Neuraminidase catalyzes the hydrolysis of α2-3, α2-6, and α2-8 linked N-acetylneuraminic acid residues from glycoproteins and oligosaccharides.

Substrate Specificity:

Product Source
Cloned from Clostridium perfringens and overexpressed in E. coli.

This product is related to the following categories:

Exoglycosidases,

Proteome Analysis

This product can be used in the following applications:

Expression Systems,

Glycan Sequencing,

Proteomics,

Recombinant Glycoprotein Expression,
Glycoprotein Analysis
- Properties & Usage
  
  Unit Definition
  One unit is defined as the amount of enzyme required to cleave > 95% of the terminal α-Neu5Ac from 1 nmol Neu5Acα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC), in 5 minutes at 37°C in a total reaction volume of 10 µl.
  Reaction Conditions
  
  1X GlycoBuffer 1
  Incubate at 37°C
  
  Storage Buffer
  
  50 mM NaCl
  20 mM Tris-HCl
  5 mM EDTA
  pH 7,5 @ 25°C
  
  Heat Inactivation
  65°C for 10 minutes
  Unit Assay Conditions
  Two fold dilutions of α2-3,6,8 Neuraminidase are incubated with 1 nmol AMC-labeled substrate and 1X GlycoBuffer 1 in a 10 µl reaction. The reaction mix is incubated at 37°C for 5 minutes. Separation of reaction products are visualized via thin layer chromatography (3).
Protocols, Manuals & Usage
- Protocols for this product
  Typical Reaction Conditions for α2-3,6,8 Neuraminidase (P0720)
  
  Endo-α-N-Acetylgalactosaminidase Application Note 1
- Usage Guidelines
  Glycobiology Unit Conversion Chart
FAQs & Troubleshooting
- FAQs
  Can I use α2-3,6,8 Neuraminidase in a double digest with Endo H(H_f) or PNGase F?
  
  Is α2-3,6,8 Neuraminidase active at higher pH levels?
  
  How much exoglycosidase should be used?
  
  Do detergents inhibit exoglycosidases/endoglycosidases?
  
  What is a good positive control for α2-3,6,8 Neuraminidase?
  
  Why have the NEB Glycosidase enzymes changed reaction buffers? What are the new reaction buffers and can I still use an enzyme with its old buffer? Where can I find the composition of the old buffers?
  
  What are Glycosidases and their uses?
  
  Is it necessary to treat my glycoprotein concomitantly with Neuraminidase and O-Glycosidase?
  
  Do the NEB Neuraminidase enzymes cleave both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) residues?
- Tech Tips
  
  Don’t forget to include Neuraminidase in a digestion with O-Glycosidase.
Citations & Technical Literature
- Brochures
  Glycoproteomics Brochure
  
  Glycoproteomics Technical Guide
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
- Articles à la une
  Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  P0720S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  P0720S_L_v1_0161603
  
  P0720S_L_v1_0171609
  
  P0720S_L_v1_0171612
  
  P0720S_L_v1_0191707
  
  P0720S_L_v1_0201710
  
  P0720S_L_v1_0201801
  
  P0720L_v1_10010266
  
  P0720L_v1_10015145
  
  P0720S_v1_10018751
  
  P0720S_v1_10026475
  
  P0720L_v1_10026474
  
  P0720S_v1_10031657
  
  P0720L_v1_10044263
  
  P0720S_v1_10044262
  
  P0720S_v1_10044661
  
  P0720L_v1_10054879
  
  P0720L_v1_10056317
  
  P0720L_v1_10061039
  
  P0720S_v1_10062665
  
  P0720L_v1_10065575
  
  P0720S_v1_10065577
  
  P0720L_v1_10075882
  
  P0720S_v1_10102998
  
  P0720S_v1_10113722
  
  P0720L_v1_10103000
  
  P0720S_v1_10117868
  
  P0720L_v1_10133654
  
  P0720L_v1_10152115
  
  P0720S_v1_10156498
  
  P0720L_v1_10169875
  
  P0720S_v1_10174000
  
  P0720L_v1_10178400
  
  P0720S_v1_10212094
  
  P0720L_v1_10227077
  
  P0720L_v1_10261345
  
  P0720S_v1_10260294
  
  P0720S_v1_10276881
  
  P0720L_v1_10288674
  
  P0720L_v1_10309573
  
  P0720S_v1_10317309
  
  P0720L_v1_10316661
  
  P0720L_v1_10322678
  
  P0720S_v1_10331523
  
  P0720L_v1_10347494
  
  P0720S_v1_10350958
  
  P0720L_v1_10354519
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  α2-3,6,8 Neuraminidase
  
  GlycoBuffer 1
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

α2-3,6,8 Neuraminidase

Product Information

Substrate Specificity:

Product Source

Properties & Usage

Unit Definition

Reaction Conditions

Storage Buffer

Heat Inactivation

Unit Assay Conditions

Protocols, Manuals & Usage

Protocols for this product

Usage Guidelines

FAQs & Troubleshooting

FAQs

Tech Tips

Citations & Technical Literature

Brochures

Citations

Product Citation Tool

Articles à la une

Quality, Safety & Legal

Quality Control Assays

Specifications & Change Notifications

Certificate of Analysis

Safety Data Sheets

Legal and Disclaimers

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α2-3,6,8 Neuraminidase

Product Information

Substrate Specificity:

Product Source

Properties & Usage

Unit Definition

Reaction Conditions

Storage Buffer

Heat Inactivation

Unit Assay Conditions

Protocols, Manuals & Usage

Protocols for this product

Usage Guidelines

FAQs & Troubleshooting

FAQs

Tech Tips

Citations & Technical Literature

Brochures

Citations

Product Citation Tool

Articles à la une

Quality, Safety & Legal

Quality Control Assays

Specifications & Change Notifications

Certificate of Analysis

Safety Data Sheets

Legal and Disclaimers

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