PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from
glycoproteins. PNGase F (Glycerol-free) is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
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Peptide: N-Glycosidase F, also known as PNGase F, is an amidase which is supplied glycerol free for optimal performance in HPLC intensive methods. PNGase F cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins (1).
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| Glycoprotein Denaturing Buffer | -20 | 10 X |
| NP-40 | -20 | 10 % |
| GlycoBuffer 2 | -20 | 10 X |
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