α1-2,4,6 Fucosidase O

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
P0749S     4       α1-2,4,6 Fucosidase O P0749SVIAL 4 1 x 0.04 ml 2,000 units/ml   GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X
P0749L     4       α1-2,4,6 Fucosidase O P0749LVIAL 4 1 x 0.2 ml 2,000 units/ml   GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X

Companion Products

• α1-2 Fucosidase
• PNGase F (Glycerol-free), Recombinant
• Rapid PNGase F

1. The enzymatic unit definition is calculated using a 37°C reaction temperature, to accommodate for simultaneous glycosidase digestions and N-glycan sequencing protocols. However, the optimal reaction temperature for α1-2,4,6 Fucosidase O is 50°C. A 37°C reaction temperature results in ~35% lower activity.
2. In order to achieve complete removal of core α1-6 fucose residues from glycans with instant labels, it may be necessary to use longer incubation times (18 hours).
3. α1-2,4,6 Fucosidase O is only active on an intact antibody if it is trimmed to the trimannosyl core.
4. Reactions may be scaled-up linearly to accommodate larger reaction volumes.

1. Wong-Madden, S.T. and Landry, D. (1995). Glycobiology. 5, 19-28.

1. α1-2,4,6 Fucosidase O Digestion of Released Labeled Glycans Protocol

• Detailed Characterization of Antibody Glycan Structure using the N-Glycan Sequencing Kit

• Glycan Analyzer

1. what-is-the-difference-between-1-2-3-4-6-fucosidase-and-1-2-4-6-fucosidase-o
2. can-1-2-4-6-fucosidase-o-cleave-fucose-residues-in-the-presence-of-instant-labelled-glycans
3. can-i-use-1-2-4-6-fucosidase-o-in-a-double-digest-with-other-exoglycosidases
4. can-1-2-4-6-fucosidase-o-cleave-1-3-fucose-residues
5. can-1-2-4-6-fucosidase-o-cleave-branched-and-complex-substrates
6. can-1-2-4-6-fucosidase-o-cleave-fucose-residues-from-an-intact-antibody
7. what-is-the-optimal-incubation-temperature-for-1-2-4-6-fucosidase-o
8. what-is-a-good-method-to-re-purify-a-glycan-or-glycopeptide-after-exoglycosidase-treatment3
9. How much exoglycosidase should be used?

• Glycoproteomics Brochure
• Glycoproteomics Technical Guide

• O'Flaherty, Roisin, Harbison, Aoife M., Hanley, Philip J., Fadda, Elisa, Rudd, Pauline, Taron, Chris (2017) Aminoquinoline fluorescent labels obstruct efficient removal of N-glycan corex J Proteome Res; 16, 4237-4243.

• Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein
• Redesigning glycosidase manufacturing quality for pharmaceutical and clinical applications

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P0749S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P0749S_L_v1_0031708
• P0749S_L_v1_0031801
• P0749S_L_v1_0031802
• P0749S_v1_10011296
• P0749S_v1_10042354
• P0749S_v1_10046126
• P0749S_v1_10056387
• P0749S_v1_10079352
• P0749L_v1_10098208
• P0749S_v1_10092835
• P0749L_v1_10100469
• P0749S_v1_10100472
• P0749L_v1_10106659
• P0749S_v1_10106656
• P0749S_v1_10122786
• P0749L_v1_10122787
• P0749S_v1_10151498
• P0749L_v1_10164134
• P0749S_v1_10164849
• P0749S_v1_10179581
• P0749L_v1_10164851
• P0749S_v1_10182662
• P0749L_v1_10184695
• P0749L_v1_10185530
• P0749S_v1_10185532
• P0749S_v1_10195158
• P0749L_v1_10195157
• P0749S_v1_10221881
• P0749L_v1_10223295
• P0749S_v1_10238121
• P0749L_v1_10239432
• P0749S_v1_10245039
• P0749L_v1_10247528
• P0749L_v1_10252787
• P0749S_v1_10255351
• P0749L_v1_10262596

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• α1-2,4,6 Fucosidase O
• GlycoBuffer 1

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.