β1-3 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-3 and, at a much lower rate, β1-6 linked galactose residues from oligosaccharides.
- Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
- Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability for up to 24 months
β1-3 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-3 and, at a much lower rate, β1-6 linked D-galactopyranosyl residues from oligosaccharides. The approximate kinetic data show > 100-fold preference for β1-3 over β1-6 linkages (1,2), and > 500-fold preference from β1-3 over β1-4 linkages (3).
Detailed Specificity: The GlcNAc(β1-6) residue is the only anomeric configuration that can effect the specificity of the enzyme enabling cleavage of the non-reducing β1-4Galactose (Fig. 1).
Product SourceCloned from Xanthomonas manihotis and expressed in E. coli at NEB (4).
The following reagents are supplied with this product:
Store at (°C) Concentration GlycoBuffer 1 -20 10 X Purified BSA -20 100 X
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