Endo F3

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
P0771S     -20       Endo F3 P0771SVIAL -20 1 x 0.03 ml 8,000 units/ml   GlycoBuffer 4 B1703SVIAL -20 1 x 1 ml 10 X

Companion Products

• Endo H
• Endo H_f
• PNGase F (Glycerol-free), Recombinant
• Rapid PNGase F
• Rapid™ PNGase F (non-reducing format)
• Endo S
• Endo D

1. The optimal pH for the cleavage of fucosylated-biantennary (i.e. porcine fibrinogen) and triantennary glycans (i.e. fetuin) is pH 4.5 (10X GlycoBuffer 4). However, fucosylated-biantennary glycans can also be cleaved at pH 6.0 (10X GlycoBuffer 3).
2. The enzyme should be used under non-denaturing conditions. Detergents, such as SDS, inhibit Endo F3 activity.
3. Reactions may be scaled-up linearly to accommodate larger reaction volumes.

1. Typical Reaction Conditions for Endo F3 Protocol (P0771)

• Endoglycosidase Selection Chart

1. Is Endo F3 tagged?  
2. What is the difference between PNGase F and Endo F3?
3. How much Endo F3 should I use to deglycosylate a glycoprotein under native conditions?
4. What is the preferred substrate for Endo F3?
5. Do detergents inhibit Endo F3 activity?
6. What is the optimal pH for Endo F3 activity?
7. How do I eliminate Endo F3 from a reaction?
8. What is the binding capacity of the Magnetic Chitin Beads used to remove Endo F3?
9. Is Endo F3 compatible with downstream analysis such as HPLC and Mass Spectrometry?
10. What are Glycosidases and their uses?

• Glycoproteomics Brochure
• Glycoproteomics Technical Guide

• Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P0771S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P0771S_L_v1_0011712
• P0771S_v1_10015255
• P0771S_v1_10037296
• P0771S_v1_10059841
• P0771S_v1_10064384
• P0771S_v1_10112216
• P0771S_v1_10152120
• P0771S_v1_10166668
• P0771S_v1_10214337
• P0771S_v1_10239661
• P0771S_v1_10261238

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Endo F3
• GlycoBuffer 4

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.