O-Glycosidase & Neuraminidase Bundle
This bundle contains 1 vial each of O-Glycosidase (NEB #P0733S) and a2-3,6,8 Neuraminidase (NEB #P0720S), which can be used simultaneously for the removal of terminal sialic acid residues and core 1 and core 3 O-glycans.
- Recombinant enzymes with no detectable endoglycosidase or other exoglycosidase contaminating activities
- Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability for up to 24 months
1 set of this bundle includes 2,000,000 units of O-Glycosidase and 2,000 units of Neuraminidase.
O-Glycosidase, also known as Endo-α-N-Acetylgalactosaminidase, catalyzes the removal of Core 1 and Core 3 O-linked disaccharides from glycoproteins.
Neuraminidase is the common name for Acetyl-neuraminyl hydrolase (Sialidase). This Neuraminidase catalyzes the hydrolysis of α2-3, α2-6, and α2-8 linked N-acetyl-neuraminic acid residues from glycoproteins and oligosaccharides.
Product SourceO-Glycosidase is cloned from Enterococcus faecalis and expressed in E.coli (1). Neuraminidase is cloned from Clostridium perfringens (2) and overexpressed in E. coli (3).
The following reagents are supplied with this product:
Store at (°C) Concentration Glycoprotein Denaturing Buffer -20 10 X NP-40 10 % GlycoBuffer 2 4 10 X
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