PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination
- Stored in 50% glycerol; optimal activity and stability for up to 24 months
- Can be used under native or denaturing conditions
- Optimized for deglycosylation of glycoproteins; leaves N-glycan core oligosaccharides intact and suitable for further analysis
Overview of Glycobiology
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Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins (1)
Product SourcePNGase F is purified from Flavobacterium meningosepticum (3) and it is free of proteases and Endo F activities.
The following reagents are supplied with this product:
Store at (°C) Concentration Glycoprotein Denaturing Buffer -20 10 X NP-40 4 10 % GlycoBuffer 2 4 10 X
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