Next Generation Sequencing Library Preparation

Successful Next Generation Sequencing (NGS) hinges on several factors, but one of the most impactful of them is the choice of library preparation workflow. As NGS matures into an essential analysis method, libraries of the highest quality are required, in yields sufficient for a wide range of applications and throughputs, from single-cell and cfDNA to PCR-free whole-genome analysis. Automation compatibility is a major consideration, too, as liquid-handling robots are becoming more accessible and commonplace..

NEBNext UltraExpress® and NEBNext® Ultra™ II: Faster workflows for better libraries

For 15 years, NEB has addressed these challenges by offering solutions that streamline sample prep workflows, minimize inputs and improve library yield and quality. With over 100 products, the NEBNext portfolio includes sample prep solutions for DNA sequencing and RNA sequencing from a wide range of sample types. The NEBNext workflow lies at the heart of the portfolio, and products are available in a convenient kit format, or as separate modules – offering flexible library prep workflows that are easily scaled and automated. Our new NEBNext UltraExpress workflows take speed and convenience even farther, generating high-quality libraries in less time with a single protocol (with a single adaptor concentration and number of PCR cycles) for all samples within the input range. The flagship NEBNext Ultra II workflow sets the standard for high-quality NGS library preparation for complex and precious samples

NEBNext reagents are currently available for next generation sequencing sample preparation on Illumina®, Oxford Nanopore Technologies® (ONT) and IonTorrent® sequencing platforms, and have already been applied to emerging sequencing platforms.


Choose Type:

Next Generation Sequencing Library Preparation includes these subcategories:
Library Preparation for Illumina
Library Preparation for Ion Torrent
Infectious Diseases & ARTIC Sequencing
DNA Fragmentation & RNA Fragmentation
RNA Depletion & mRNA Enrichment
Methylome Analysis
Microbiome DNA Enrichment
NEBNext® Multiplex Oligos (Adaptors & Primers)
NGS Library Quantitation
Automation for NEBNext® NGS Library Prep
Small RNA Library Preparation
Application Notes for Next Generation Sequencing Library Preparation
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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