NEBNext^® Ultra™ II Ligation Module 

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E7595S     -20       NEBNext® Ligation Enhancer E7374AVIAL -20 1 x 0.024 ml Not Applicable   NEBNext® Ultra™ II Ligation Master Mix E7648AVIAL -20 1 x 0.72 ml Not Applicable
E7595L     -20       NEBNext® Ligation Enhancer E7374AAVIAL -20 1 x 0.096 ml Not Applicable   NEBNext® Ultra™ II Ligation Master Mix E7648AAVIAL -20 3 x 0.96 ml Not Applicable

Companion Products

• NEBNext® Ultra™ II End Repair/dA-Tailing Module
• NEBNext® Ultra™ II FS DNA Module
• NEBNext® Ultra™ II Q5® Master Mix

1. Protocol for use with NEBNext Ultra II End Repair/dA-Tailing Module (E7546) and NEBNext Ultra II Ligation Module (E7595)
2. Protocol for use with NEBNext Ultra II FS DNA Module (E7810) and NEBNext Ultra II Ligation Module (E7595)
3. Where can I find protocols for using NEBNext^® DNA Library Prep reagents?

1. What is the difference between the three NEBNext Ligation Modules?
2. How do I obtain the NEBNext Adaptor and USER enzyme for use with the NEBNext Ultra II Ligation Module?

NEBNext Product Citations Search Tool

Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7595S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7595S_v1_10011910
• E7595L_v1_10012568
• E7595L_v1_10013960
• E7595L_v1_10015110
• E7595S_v1_10019845
• E7595L_v1_10008035
• E7595S_v1_10008032
• E7595L_v1_10026681
• E7595L_v1_10028917
• E7595S_v1_10024801
• E7595L_v1_10024804
• E7595S_v1_10035780
• E7595L_v1_10040898
• E7595L_v1_10040983
• E7595L_v1_10041323
• E7595S_v1_10041331
• E7595L_v1_10044728
• E7595L_v1_10038057
• E7595S_v1_10048902
• E7595S_v1_10051819
• E7595L_v1_10054275
• E7595L_v1_10055692
• E7595L_v1_10049274
• E7595S_v0_10049271
• E7595L_v1_10063918
• E7595S_v1_10063921
• E7595S_v1_10059715
• E7595L_v1_10059718
• E7595L_v1_10072785
• E7595S_v1_10072787
• E7595L_v1_10071194
• E7595L_v1_10072996
• E7595S_v1_10071191
• E7595L_v1_10075491
• E7595S_v1_10075354
• E7595L_v1_10077645
• E7595S_v1_10078041
• E7595L_v1_10078751
• E7595S_v1_10079850
• E7595L_v1_10082690
• E7595S_v1_10089681
• E7595L_v1_10093888
• E7595L_v1_10099973
• E7595S_v1_10094525
• E7595L_v1_10094528
• E7595S_v1_10109302
• E7595L_v1_10113786
• E7595L_v1_10115159
• E7595L_v1_10109305
• E7595L_v1_10129168
• E7595S_v1_10120045
• E7595L_v1_10140774
• E7595L_v1_10142711
• E7595S_v1_10133474
• E7595L_v1_10142799
• E7595L_v1_10133471
• E7595L_v1_10157462
• E7595S_v1_10148992
• E7595L_v1_10166902
• E7595S_v1_10170491
• E7595L_v1_10148989
• E7595S_v1_10178345
• E7595L_v1_10179531
• E7595L_v1_10169184
• E7595S_v1_10169187
• E7595L_v1_10188347
• E7595S_v1_10189275
• E7595S_v1_10195610
• E7595L_v1_10196502
• E7595S_v1_10183575
• E7595L_v1_10183572
• E7595L_v1_10200430
• E7595S_v1_10200433
• E7595L_v1_10217726
• E7595S_v1_10222536
• E7595L_v1_10231776
• E7595L_v1_10222533
• E7595S_v1_10239388
• E7595L_v1_10250734
• E7595L_v1_10250732
• E7595L_v1_10259610

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext^® Ligation Enhancer
• NEBNext^® Ultra^™ II Ligation Master Mix

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.