This article traces molecular cloning’s history and progress, highlighting how improved enzymes, assembly methods, and DNA design tools have advanced cloning efficiency, precision and applications.
NEBaseChanger® Primer Design Toolチュートリアルビデオでは、NEBaseChangerを用いて部位特異的および複数部位への変異導入用のプライマーをデザインする方法を説明します。このツールは、NEB Q5®およびNEBuilder® HiFi DNA Assemblyアプリケーションに最適です。
Explore NEB Expressions, featuring a precision medicine case study from discovery to targeted therapy, cell-free dbDNA synthesis, enzymatic solutions for circular RNA enrichment, conservation efforts in Costa Rica, RNA workflow products and the 2026 Passion in Science Awards.
RNase 4 Supports Precise Characterization of mRNA 5´ Cap Structures
Posted on 2026年9月14日
By
Iris Martinez, Illustration by Tasha José
& Reviewed by Development Scientist Jeremy C. Henderson, Ph.D.
Critical quality attributes (CQAs) of mRNA vaccines, including sequence identity, 5′ capping efficiency, and 3′ poly(A) tail length, require thorough characterization. RNase 4 can be used to digest long mRNA molecules into oligonucleotide fragments that are amenable to analysis by LC-MS, capillary electrophoresis (CE), or denaturing gel-based methods, thereby enabling detailed assessment of mRNA therapeutic safety and content.
Highly Efficient Peptide Binder Discovery Using mRNA Display
Posted on 2026年8月24日
By
Nicole Kelesoglu
& Illustration by Tasha Jose
In this interview, PeptiFinder Biotech scientists share why mRNA Display is such a powerful platform for peptide therapeutics discovery, where they see this field going, and how enzyme engineering and workflow optimization could further expand the chemical space accessible to mRNA display.
RNase III Protocol Modification Can Enable Safe dsRNA Removal from IVT Samples
Posted on 2026年7月20日
By
Iris Martinez, Illustrations by Tasha José
High-quality and pure RNA is a requirement for RNA therapeutics to avoid unwanted immune responses, not just a “nice to have.” In vitro transcription can be optimized, but additional cleanup may be needed. Enzymatic removal of dsRNA using RNase III preserves ssRNA integrity when refined using a protocol developed by NEB scientists.
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