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NEBNext UltraExpress™ FS DNA Library Prep Kit

The NEBNext UltraExpress™ FS DNA Library Prep Kit is a single enzyme mix for enzymatic fragmentation, end prep and dA-tailing. This enables the generation of high-quality, high-yield DNA libraries from a broad input range in less than two hours using a single protocol.

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Authenticase is a mix of structure-specific nucleases that targets mismatch and indel regions (1-10 bp) on double-stranded DNA. It can be used as an error-correction reagent in oligo-based PCR gene assembly. It can also replace T7 Endonuclease I for genome editing efficiency assessment.

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NEBNext UltraExpress RNA Library Prep Kit
NEBNext UltraExpress™ RNA Library Prep Kit

The NEBNext UltraExpress™ RNA Library Prep Kit has a 3-hour library prep protocol & creates high-quality directional RNA libraries in a single day when paired with poly(A) mRNA enrichment or rRNA depletion kits. The kit features a single protocol, master mixed reagents, reduced incubation times, & fewer cleanup steps.

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Review NEB’s Latest Research
Feature Article

Read about how NEB scientists use Golden Gate Assembly, data-optimized assembly design (DAD) and online tools to design one-pot assemblies of medium to high complexity genomes.

Featured Video

NEB scientists have shown that RNase I has Ca2+-dependent double-stranded-RNase activity, and DNA:RNA hybridase activity.

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App Note

Learn about our one-day in vitro workflow of Golden Gate Assembly, RCA, and cell-free protein expression for high-throughput screening, generating custom protein variants.

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New Publication

An in-vitro strategy developed by NEB scientists expands cytosine deaminase applications and uncovers novel deaminases. They introduce a non-destructive 5-methylcytosine sequencing method (SEM-seq) utilizing a newly identified DNA deaminase.

Web Tool

The latest version of NEBaseChanger® is available to help design site-directed mutagenesis primers. Compatible with ambiguous bases, substitute based on amino acid position, batch upload for multiple primer sets and add mutations to primer tails.

New Publication

In this issue of NEB Expressions, explore DNA construction and gene synthesis with NEBridge® Golden Gate Assembly and Authenticase for error correction. Achieve precision in FFPE DNA library prep using NEBNext®, and much more!

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