For superior performance, the NEBNext FFPE DNA Repair v2 Module (NEB #E7360) is now available.
Kits for FFPE DNA library prep are now available, with or without enzymatic DNA fragmentation (NEBNext UltraShear™ FFPE DNA Library Prep Kit NEB #E6655 and NEBNext FFPE DNA Library Prep Kit NEB #E6650).
FFPE DAMAGE TYPE | REPAIRED BY THE FFPE DNA ENZYME REPAIR MIX? |
Deamination of cytosine to uracil | Yes |
Nicks and gaps | Yes |
Oxidized bases | Yes |
Blocked 3´ ends | Yes |
DNA fragmentation | No |
DNA-protein crosslinks | No |
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