Home Next Generation Sequencing Library Preparation Products NEBNext^® Poly(A) mRNA Magnetic Isolation Module

NEBNext^® Poly(A) mRNA Magnetic Isolation Module

An Express protocol option is now available in the product manual, providing substantial time savings with a more streamlined workflow.

Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails.

This product is compatible with total RNA inputs of 1-5µg. For input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).

Catalog #	Concentration	Size	List Price	Your Price
E7490S	Not Applicable	24 reactions	$76.00	Sign In Sign In
E7490L	Not Applicable	96 reactions	$275.00	Sign In Sign In

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Product Information

The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from 1-5µg of previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.

Note that for input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).

This product is related to the following categories:: RNA Depletion & mRNA Enrichment Products,; RNA Library Prep for Illumina,; Next Generation Sequencing Library Preparation Products

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

E7490S

NEBNext RNA Binding Buffer (2X)	E7492AVIAL	4	1 x 7.2 ml	Not Applicable
NEBNext Wash Buffer	E7493AVIAL	4	1 x 28.8 ml	Not Applicable
Nuclease-free Water	E7495AVIAL	4	1 x 1.2 ml	Not Applicable
NEBNext Tris Buffer	E7496AVIAL	4	1 x 6 ml	Not Applicable
NEBNext Oligo d(T)25 Beads	E7499AVIAL	4	1 x 0.48 ml	Not Applicable

E7490L

NEBNext RNA Binding Buffer (2X)	E7492AAVIAL	4	1 x 28.8 ml	Not Applicable
NEBNext Wash Buffer	E7493AAVIAL	4	2 x 57.6 ml	Not Applicable
Nuclease-free Water	E7495AAVIAL	4	1 x 4.8 ml	Not Applicable
NEBNext Tris Buffer	E7496AAVIAL	4	1 x 24 ml	Not Applicable
NEBNext Oligo d(T)25 Beads	E7499AAVIAL	4	1 x 1.92 ml	Not Applicable

Properties & Usage
Materials Required but not Supplied
- 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
- Magnetic rack or plate (e.g., NEBNext^® Magnetic Separation Rack (NEB #S1515S), Alpaqua ^® 96S Super Magnet Plate (#A001322), or equivalent)
- Thermal cycler or heat block
- Bioanalyzer^® (Agilent Technologies, Inc.) or similar instrument and consumables.
Advantages and Features

Application Features
Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.

Protocols, Manuals & Usage
- Protocols
  Where can I find protocols for using NEBNext^® RNA Library Prep reagents?
- Manuals
  The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
  
  manualE7490
- Usage Guidelines
  Getting Started with RNA-Sequencing (RNA-Seq)
FAQs & Troubleshooting
- FAQs
  Can I isolate poly(A)⁺ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
  
  Do I need two rounds of binding?
  
  Can I use the Oligo (dT)₂₅ Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?
Citations & Technical Literature
- Citations
  
  NEBNext Product Citations Search Tool
  
  Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  E7490S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  E7490G_v0_10010544
  
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  E7490S_v1_10260104
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  NEBNext RNA Binding Buffer (2X)
  
  NEBNext Wash Buffer
  
  Nuclease-free Water
  
  NEBNext Tris Buffer
  
  NEBNext Oligo d(T)25 Beads
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Licenses
  This product is for research use only, not for therapeutic use, and for use only in nucleic acid purification as part of sample preparation prior to sequencing.

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

Product Information

Reagents Supplied

Properties & Usage

Materials Required but not Supplied

Advantages and Features

Application Features

Protocols, Manuals & Usage

Protocols

Manuals

Usage Guidelines

FAQs & Troubleshooting

FAQs

Citations & Technical Literature

Citations

NEBNext Product Citations Search Tool

Quality, Safety & Legal

Quality Control Assays

Specifications & Change Notifications

Certificate of Analysis

Safety Data Sheets

Legal and Disclaimers

Licenses

Other Products You May Be Interested In

NEBNext® Magnesium RNA Fragmentation Module

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NEBNext^® Poly(A) mRNA Magnetic Isolation Module

NEBNext^® Magnesium RNA Fragmentation Module