NEBNext® Enzymatic 5hmC-seq Kit

NEBNext® Enzymatic 5hmC-seq (E5hmC-seq™) is a new method for the specific detection of 5hmC at the single base level.

5hmC is detected using a two-step enzymatic conversion workflow that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC, after Illumina® sequencing. The kit has an input range of 0.1–200 ng and includes NEBNext Ultra™ II library prep reagents and the E5hmC-seq Adaptor.

Note that index primers are not included and must be purchased separately (NEBNext Primers for Epigenetics, NEB #E3392, NEB #E3404).

  • High sensitivity of detection of 5hmC
  • 0.1–200 ng input range
  • Even GC coverage
  • High-efficiency library preparation
  • E5hmC-seq and EM-seq data can be combined
  • Conversion module also available separately (NEB #E3365)

View or download extensive performance data below or in our Data Supplement.

Catalog # Concentration Size List Price Your Price Quantity
E3350S Not Applicable 24 reactions $820.00
E3350L Not Applicable 96 reactions $3,110.00
Please enter a quantity for at least one size

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