NEBNext® Ultra™ II Q5® Master Mix

NEBNext Ultra II Q5® Master Mix is the most recent NEBNext formulation of Q5 High Fidelity DNA Polymerase, optimized for amplification of NGS libraries. This hot start formulation is designed for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, and further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.

NEBNext Ultra II Q5 Master Mix is also included as a component in all NEBNext Ultra II kits, for DNA and RNA library preparation.

  • Optimized for high yields in NGS library amplification
  • Minimizes GC bias, with superior performance across the GC spectrum
  • Ultra-high-fidelity amplification with Q5, the highest-fidelity polymerase (1)
  • Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
  • Bulk format available: M0544X = 500 reactions in 1 x 12.5 ml bottle


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Catalog # Concentration Size List Price Your Price Quantity
M0544S 2 X 50 reactions $102.00
M0544L 2 X 250 reactions $407.00
M0544X 2 X 500 reactions $774.00
Please enter a quantity for at least one size

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