The qPCR standards included in this product are intended for use with libraries prepped for sequencing on Illumina® instruments.
NEBNext Library Quant DNA Standards are a set of six standards, defining a curve from 100 pM to 0.001 pM, intended for use with the NEBNext Library Quant Master Mix included in the NEBNext Library Quant Kit for Illumina (NEB #E7630).
Additional volume of the same standards included in the NEBNext Library Quant Kit for Illumina
qPCR-based library quant based on either four or six standards
For use with the NEBNext Library Quant Master Mix included in the NEBNext Libarary Quant Kit for Illumina, this product includes six tubes of standards (100 pM to 0.001 pM) providing extra volume for users who require it. Protocols included in the (NEB #E7630) product manual detail the process for setting up standard curves using either four or six standards, building flexibility into the workflow.
The NEBNext Library Quant Kit for Illumina offers a streamlined protocol and reduced lot-to-lot variability as compared to similar library quantification kits.
Figure 1: Typical amplification and standard curves for the NEBNext Library Quant Kit
Typical results from the NEBNext Library Quant Kit on a Bio-Rad CFX96 Touch (A) and an Applied Biosystems 7500 Fast real-time qPCR instrument (B). Amplification curves are shown on the left and resulting standard curves on the right. All default settings were used on both platforms. ABI 7500 Fast assay included 1X ROX (Low Concentration) and the ROX normalization.
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Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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