The NEBNext High-Input Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from high inputs (5-50 µg per 0.2 ml reaction) of previously isolated total RNA, using oligo d(T)25-coupled paramagnetic beads. Intact mRNA can be obtained in approximately one hour, and eluted in small volumes.
The NEBNext High-Input Poly(A) mRNA Magnetic Isolation Module isolates intact poly(A)+ RNA from high inputs (5-50 µg per 0.2 ml reaction) of previously isolated total RNA. The technology is based on the coupling of oligo d(T)25 to paramagnetic beads, which is then used as the solid support for the direct binding of poly(A)+ RNA. The procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes, thereby eliminating the need for precipitation of the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA, which is representative of the mRNA population of the original sample, can be obtained in approximately one hour.
For inputs less than 5 µg, the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) is recommended.
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