NEBNext® RSV Primer Module is intended for use in cDNA synthesis, amplification, and library preparation upstream of Respiratory Syncytial Virus (RSV) sequencing. The Primer Module can be used upstream of both Illumina® and Oxford Nanopore Technologies® sequencing, when paired with LunaScript Multiplex One-Step RT-PCR Kit and a compatible library prep solution (e.g., NEBNext UltraExpress FS DNA Library Prep Kit or ONT library prep reagents).
Full-length coverage of RSV A and RSV B
Intentionally designed, balanced, and functionally optimized primer sequences
Flexibility to use with Illumina® or Oxford Nanopore Technologies® platforms
Note: This product is intended for research-use only.
Product Information
RSV is a virus that commonly infects infants and the elderly, causing severe respiratory symptoms, while leading to milder illness in older children and young adults. For laboratories interested in sequencing the RSV genome, the NEBNext RSV Primer Module contains a curated set of primer mixes for use in RT-PCR upstream of NGS library preparation. Downstream, the libraries are compatible with Illumina or ONT chemistries. Refer to the product manual for the required materials not supplied for your sequencing platform of choice.
The NEBNext RSV Primer Module has undergone extensive QCs to ensure that the primer mixes are balanced, well-aligned with modern strains of RSV, and free of cross contamination. In-house testing includes the full recommended workflow, combining RT-PCR, NGS library prep, and sequencing followed by analysis of sequencing metrics.
Due to its modular nature, and compatibility with a range of downstream sequencing platforms, the NEBNext RSV Primer Module provides flexibility for maximal ease of use. Not only is the NEBNext RSV Primer Module compatible with both Illumina and ONT sequencers, the workflow is automation friendly.
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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