EpiMark^® Hot Start Taq DNA Polymerase

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
NEB # Stored at (°C) M0490S -20 Component Name Component # Stored at (°C) Amount Concentration EpiMark® Hot Start Taq DNA Polymerase M0490SVIAL -20 1 x 0.025 ml 5,000 units/ml EpiMark® Hot Start Taq Reaction Buffer Pack B0490SVIAL -20 1 x 2 ml 5 X
NEB # Stored at (°C) M0490L -20 Component Name Component # Stored at (°C) Amount Concentration EpiMark® Hot Start Taq DNA Polymerase M0490LVIAL -20 1 x 0.125 ml 5,000 units/ml EpiMark® Hot Start Taq Reaction Buffer Pack B0490SVIAL -20 3 x 2 ml 5 X

Application Features

• PCR from bisulfite-converted DNA

Companion Products

• Magnesium Chloride (MgCl₂) Solution
• Deoxynucleotide (dNTP) Solution Set
• EpiMark® Methylated DNA Enrichment Kit
• Deoxynucleotide (dNTP) Solution Mix

Materials Sold Separately

• EpiMark® Hot Start Taq Reaction Buffer Pack

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact. . 127, 1550-1557.
2. Kaledin, A.S., Slyusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya . 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCR Methods And Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res. . 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques . 15, 372-374.

1. EpiMark® Hot Start Taq DNA Polymerase Guidelines for PCR (M0490)

• Activity of Restriction Enzymes in PCR Buffers

• Tm Calculator

1. What ends will my PCR products have?
2. What are Hot Start and WarmStart® polymerases and when would I use them?
3. How should I determine the appropriate annealing temperature for my reaction?
4. My PCR results are not as expected. Where can I find troubleshooting help?
5. What should the final primer concentration be in my PCR?
6. Is this Taq DNA Polymerase product compatible with other NEB Buffers?
7. What are the general recommendations for designing primers for bisulfite-treated/deaminated DNA?

• Est?ve P., Zhang G., Ponnaluri V., Kanneganti (2015) Binding of 14-3-3 reader proteins to phosphorylated DNMT1 facilitates aberrant DNA methylation and gene expression Nucleic Acids Res; PubMedID: 26553800, DOI: 10.1093/nar/gkv1162

• Using aptamers to control enzyme activity: Hot Start Taq and beyond

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0490S_L_v3

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0490S_L_v1_0111606
• M0490S_L_v1_0111612
• M0490S_L_v1_0111712
• M0490L_v1_10013931
• M0490S_v1_10013943
• M0490S_v1_10035715
• M0490L_v1_10040455
• M0490S_v1_10047882
• M0490L_v1_10051222
• M0490S_v1_10053523
• M0490S_v1_10061336
• M0490L_v2_10067928
• M0490S_v2_10069220
• M0490L_v2_10093511
• M0490S_v2_10100849
• M0490L_v2_10112808
• M0490S_v2_10122332
• M0490L_v2_10127659
• M0490L_v2_10130878
• M0490L_v2_10134794
• M0490S_v2_10136452
• M0490L_v2_10154962
• M0490S_v2_10156485
• M0490S_v2_10171017
• M0490L_v2_10184735
• M0490L_v2_10191606
• M0490S_v2_10208806
• M0490S_v2_10230754
• M0490L_v2_10230755
• M0490S_v3_10264681
• M0490L_v3_10273675
• M0490S_v3_10288811
• M0490L_v3_10290017
• M0490S_v3_10302064
• M0490L_v3_10308367
• M0490L_v3_10322691
• M0490S_v3_10314633
• M0490S_v3_10343830

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• EpiMark^® Hot Start Taq DNA Polymerase
• EpiMark^® Hot Start Taq Reaction Buffer Pack

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use EpiMark Hot Start Taq DNA Polymerase for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com. 

Trademarks

NEW ENGLAND BIOLABS®, EPIMARK® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
IGEPAL^® is a registered trademark of Rhodia  Operations.
TWEEN^® is a registered  trademark of Uniqema americas LLC.