NEBNext^® Ultra^™ II FS DNA Module

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E7810S     -20       NEBNext® Ultra™ II FS Enzyme Mix E7806AVIAL -20 1 x 0.048 ml Not Applicable   NEBNext® Ultra™ II FS Reaction Buffer E7807AVIAL -20 1 x 0.168 ml Not Applicable   TE Buffer (1X) E7808AVIAL -20 1 x 1.1 ml 1 X
E7810L     -20       NEBNext® Ultra™ II FS Enzyme Mix E7806AAVIAL -20 1 x 0.192 ml Not Applicable   NEBNext® Ultra™ II FS Reaction Buffer E7807AAVIAL -20 1 x 0.672 ml Not Applicable   TE Buffer (1X) E7808AAVIAL -20 1 x 4.3 ml 1 X

1. Protocol for use with NEBNext® Ultra™ II FS DNA Module (E7810)

1. When preparing samples using the NEBNext Ultra II FS DNA Library Prep Kit can my input DNA be in EDTA-containing solutions
2. What do I do if I see a precipitate in the Ultra II FS Reaction Buffer
3. Following the Fragmentation and End Prep steps of the NEBNext Ultra II FS workflow can the reactions be kept at 4C
4. Do I really need to vortex the Ultra II FS Enzyme Mix
5. What is the concentration of my index primers
6. Which NEBNext ligation module should be used in conjunction with the NEBNext Ultra II FS DNA Module
7. Can I use the NEBNext Ultra II FS DNA Module for bisulfite conversion and EMSeq workflows?
8. Does the fragmentation reagent included in NEBNext Ultra^® II FS DNA kits contain NEBNext dsDNA Fragmentase^®?
9. Is the NEBNext^® Ultra^™ II FS DNA Library Prep Kit for Illumina^® compatible with all sample types?

NEBNext Product Citations Search Tool

Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.

• Improving Enzymatic DNA Fragmentation for Next Generation Sequencing Library Construction

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7810S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7810S_v1_10011344
• E7810S_v1_10015881
• E7810L_v1_10017671
• E7810S_v1_10017704
• E7810S_v1_10024726
• E7810L_v1_10014573
• E7810S_v1_10029757
• E7810L_v1_10027438
• E7810S_v1_10033280
• E7810L_v1_10037420
• E7810L_v1_10040184
• E7810S_v1_10040466
• E7810S_v1_10045344
• E7810L_v1_10046077
• E7810L_v1_10048607
• E7810S_v1_10037416
• E7810L_v1_10052033
• E7810S_v1_10046476
• E7810L_v1_10059343
• E7810S_v1_10055935
• E7810L_v1_10065015
• E7810L_v1_10067024
• E7810S_v1_10072100
• E7810L_v1_10072641
• E7810L_v1_10068321
• E7810L_v1_10080426
• E7810S_v1_10080144
• E7810L_v1_10085359
• E7810S_v1_10068317
• E7810S_v1_10094293
• E7810S_v1_10097196
• E7810L_v1_10099632
• E7810S_v1_10086819
• E7810L_v1_10103024
• E7810S_v1_10099327
• E7810L_v1_10099331
• E7810L_v1_10113554
• E7810L_v1_10118441
• E7810S_v1_10118446
• E7810S_v1_10121659
• E7810L_v1_10111542
• E7810L_v1_10134174
• E7810S_v1_10127265
• E7810L_v1_10127290
• E7810L_v1_10150358
• E7810S_v1_10144440
• E7810S_v1_10154386
• E7810L_v1_10156017
• E7810S_v1_10152763
• E7810L_v1_10168095
• E7810S_v1_10171968
• E7810L_v1_10174542
• E7810S_v1_10177827
• E7810L_v1_10161376
• E7810L_v1_10174782
• E7810S_v1_10174757
• E7810L_v1_10186490
• E7810S_v1_10186045
• E7810L_v1_10196355
• E7810S_v1_10196358
• E7810L_v1_10187215
• E7810S_v1_10187188
• E7810L_v1_10221926
• E7810S_v1_10209547
• E7810L_v1_10221883
• E7810L_v1_10231629
• E7810S_v1_10231607
• E7810L_v1_10245802
• E7810L_v1_10255007
• E7810S_v1_10245779
• E7810L_v1_10259609

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext^® Ultra^™ II FS Enzyme Mix
• NEBNext^® Ultra^™ II FS Reaction Buffer
• TE Buffer (1X)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.