Next Generation Sequencing Library Preparation


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Next Generation Sequencing Library Preparation

Library Preparation for Illumina

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NEBNext i701 Primer

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NEBNext® ARTIC SARS-CoV-2 RT-PCR Module

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NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat)

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NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® Magnetic Separation Rack

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® RNA Depletion Core Reagent Set

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NEBNext® RNA Depletion Core Reagent Set with RNA Sample Purification Beads

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NEBNext® rRNA Depletion Kit (Bacteria)

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NEBNext® rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads

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NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat)

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NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads

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NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module

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NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II Q5® Master Mix

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NEBNext® Ultra II RNA First Strand Synthesis Module

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NEBNext® Ultra II RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

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NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)

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NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)

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NEBNext® Ultra™ II DNA PCR-free Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II FS DNA PCR-free Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II DNA PCR-free Library Prep Kit for Illumina®

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NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina®

Library Preparation for Ion Torrent

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NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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NEBNext® Fast DNA Library Prep Set for Ion Torrent™

Library Preparation for Oxford Nanopore Technologies

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NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing

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NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)

Infectious Diseases & ARTIC Sequencing

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NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®)

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NEBNext® ARTIC SARS-CoV-2 RT-PCR Module

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NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)

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NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)

DNA Fragmentation & RNA Fragmentation

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Magnesium Chloride (MgCl2) (200 mM)

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NEBNext® dsDNA Fragmentase®

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II FS DNA Module

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NEBNext® dsDNA Fragmentase® Reaction Buffer v2

RNA Depletion & mRNA Enrichment

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NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat)

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NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

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NEBNext® Magnetic Separation Rack

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® RNA Depletion Core Reagent Set

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NEBNext® RNA Depletion Core Reagent Set with RNA Sample Purification Beads

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NEBNext® rRNA Depletion Kit (Bacteria)

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NEBNext® rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads

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NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat)

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NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads

Methylome Analysis

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EpiMark® 5-hmC and 5-mC Analysis Kit

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EpiMark® Methylated DNA Enrichment Kit

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EpiMark® N6-Methyladenosine Enrichment Kit

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EpiMark® Nucleosome Assembly Kit

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EpiMark® Hot Start Taq DNA Polymerase

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NEBNext® Enzymatic Methyl-seq Kit

Microbiome DNA Enrichment

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NEBNext® Magnetic Separation Rack

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NEBNext® Microbiome DNA Enrichment Kit

NEBNext® Multiplex Oligos (Adaptors & Primers) 

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NEBNext Index 41 Primer for Illumina

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NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs)

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NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1)

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NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 2)

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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 3)

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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs)

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NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 1)

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NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 4)

FFPE DNA Repair

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NEBNext® FFPE DNA Repair Mix

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NEBNext® Library Quant DNA Standards

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NEBNext® Library Quant Kit for Illumina®

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NEBNext® Magnetic Separation Rack

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NGS Library Quantitation

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NEBNext® Library Dilution Buffer

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NEBNext® Library Quant DNA Standards

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NEBNext® Library Quant Kit for Illumina®

Automation for NEBNext® NGS Library Prep

Small RNA Library Preparation

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NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

Library Preparation for Illumina
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NEBNext® Magnetic Separation Rack

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II DNA PCR-free Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II FS DNA PCR-free Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II DNA PCR-free Library Prep Kit for Illumina®

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NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina®

DNA Library Prep for Illumina

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NEBNext® Magnetic Separation Rack

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II Q5® Master Mix

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NEBNext® Ultra™ II DNA PCR-free Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II FS DNA PCR-free Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II DNA PCR-free Library Prep Kit for Illumina®

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NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina®

RNA Sample Prep for Illumina

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NEBNext i701 Primer

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NEBNext® ARTIC SARS-CoV-2 RT-PCR Module

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NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat)

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NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® Magnetic Separation Rack

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® RNA Depletion Core Reagent Set

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NEBNext® RNA Depletion Core Reagent Set with RNA Sample Purification Beads

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NEBNext® rRNA Depletion Kit (Bacteria)

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NEBNext® rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads

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NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat)

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NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads

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NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module

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NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II Q5® Master Mix

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NEBNext® Ultra II RNA First Strand Synthesis Module

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NEBNext® Ultra II RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

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NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)

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NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)

Library Preparation for Ion Torrent
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NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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NEBNext® Fast DNA Library Prep Set for Ion Torrent™

Library Preparation for Oxford Nanopore Technologies
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NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing

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NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)

Infectious Diseases & ARTIC Sequencing
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NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®)

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NEBNext® ARTIC SARS-CoV-2 RT-PCR Module

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NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)

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NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)

NEBNext® ARTIC products for SARS-CoV-2 sequencing

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NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®)

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NEBNext® ARTIC SARS-CoV-2 RT-PCR Module

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NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)

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NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)

DNA Fragmentation & RNA Fragmentation
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Magnesium Chloride (MgCl2) (200 mM)

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NEBNext® dsDNA Fragmentase®

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II FS DNA Module

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NEBNext® dsDNA Fragmentase® Reaction Buffer v2

RNA Depletion & mRNA Enrichment
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NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat)

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NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

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NEBNext® Magnetic Separation Rack

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® RNA Depletion Core Reagent Set

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NEBNext® RNA Depletion Core Reagent Set with RNA Sample Purification Beads

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NEBNext® rRNA Depletion Kit (Bacteria)

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NEBNext® rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads

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NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat)

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NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads

Methylome Analysis
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EpiMark® 5-hmC and 5-mC Analysis Kit

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EpiMark® Methylated DNA Enrichment Kit

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EpiMark® N6-Methyladenosine Enrichment Kit

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EpiMark® Nucleosome Assembly Kit

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EpiMark® Hot Start Taq DNA Polymerase

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NEBNext® Enzymatic Methyl-seq Kit

Microbiome DNA Enrichment
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NEBNext® Magnetic Separation Rack

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NEBNext® Microbiome DNA Enrichment Kit

NEBNext® Multiplex Oligos (Adaptors & Primers) 
Close

NEBNext Index 41 Primer for Illumina

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NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs)

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NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1)

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NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 2)

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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 3)

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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs)

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NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 1)

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NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 4)

FFPE DNA Repair
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NEBNext® FFPE DNA Repair Mix

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NEBNext® Library Quant DNA Standards

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NEBNext® Library Quant Kit for Illumina®

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NEBNext® Magnetic Separation Rack

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NGS Library Quantitation
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NEBNext® Library Dilution Buffer

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NEBNext® Library Quant DNA Standards

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NEBNext® Library Quant Kit for Illumina®

Automation for NEBNext® NGS Library Prep
Small RNA Library Preparation
Close

NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

Close

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

Videos

  • BeckmanAutomation_UltraWebinar_thumb_0715

    Biomek Automated NEBNEXT® Ultra Directional RNA Library Prep Kit for Illumina

    Learn how Biomek automation of NEBNext RNA library preparation enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Zach Smith from Beckman Coulter describes strand-specific RNA library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and discusses Biomek automation of this method.

  • BeckmanAutomation_ProductivityWebinar_thumb_0715

    Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent® Sequencing

    Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Joanna Hamilton from Dartmouth Genomics & Molecular Biology Shared Resource, Lynne Apone from New England Biolabs and Zach Smith from Beckman Coulter describe the NEBNext Fast Fragmentation & DNA Library Prep kit for Ion Torrent, automation of this method and implementation in multiple workflows in a sequencing core lab.