Next Generation Sequencing Library Preparation
Successful Next Generation Sequencing (NGS) hinges on several factors, but one of the most impactful of them is the choice of library preparation workflow. As NGS matures into an essential analysis method, libraries of the highest quality are required, in yields sufficient for a wide range of applications and throughputs, from single-cell and cfDNA to PCR-free whole-genome analysis. Automation compatibility is a major consideration, too, as liquid-handling robots are becoming more accessible and commonplace.
NEBNext UltraExpress® and NEBNext® Ultra™ II: Faster workflows for better libraries
For 15 years, NEB has addressed these challenges by offering solutions that streamline sample prep workflows, minimize inputs and improve library yield and quality. With over 100 products, the NEBNext portfolio includes sample prep solutions for DNA sequencing and RNA sequencing from a wide range of sample types. The NEBNext workflow lies at the heart of the portfolio, and products are available in a convenient kit format, or as separate modules – offering flexible library prep workflows that are easily scaled and automated. Our new NEBNext UltraExpress workflows take speed and convenience even further, generating high-quality libraries in less time with a single protocol (with a single adaptor concentration and number of PCR cycles) for all samples within the input range. The flagship NEBNext Ultra II workflow sets the standard for high-quality NGS library preparation for complex and precious samples.
NEBNext reagents are currently available for next generation sequencing sample preparation on Illumina®, Oxford Nanopore Technologies® (ONT) and IonTorrent® sequencing platforms, and library quantitation for Illumina and Ultima Genomics®.
What’s next for your NGS Library Prep? We’ve got you covered!
Choose Product:
Library Preparation for Illumina
Library Preparation for Ion Torrent
Library Preparation for Oxford Nanopore Technologies
Infectious Diseases & ARTIC Sequencing
DNA Fragmentation
Library Preparation for Ultima Genomics
Library Preparation for Element Biosciences
RNA Depletion & mRNA Enrichment
Methylome Analysis
Microbiome DNA Enrichment
NEBNext® Multiplex Oligos (Adaptors & Primers)
FFPE DNA
NGS Library Quantitation
Automation for NEBNext® NGS Library Prep
Small RNA Library Preparation
DNA Library Prep for Illumina
RNA Sample Prep for Illumina
Influenza Sequencing
NEBNext® ARTIC products for SARS-CoV-2 sequencing
Respiratory Syncytial Virus (RSV) Sequencing
Choose Type:
- Library Preparation for Illumina
- Library Preparation for Ion Torrent
- Library Preparation for Oxford Nanopore Technologies
- Infectious Diseases & ARTIC Sequencing
- DNA Fragmentation
- Library Preparation for Ultima Genomics
- Library Preparation for Element Biosciences
- RNA Depletion & mRNA Enrichment
- Methylome Analysis
- Microbiome DNA Enrichment
- NEBNext® Multiplex Oligos (Adaptors & Primers)
- FFPE DNA
- NGS Library Quantitation
- Automation for NEBNext® NGS Library Prep
- Small RNA Library Preparation
- Obtain superior NGS library performance with lower input amounts using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
- Increased transcription detection with the NEBNext Single Cell/Low Input RNA Library Prep Kit
- Monarch HMW DNA Extraction Kit improves sample preparation for Oxford Nanopore Technologies sequencing of malaria parasites
- High-yield, Scalable Library Preparation with the NEBNext® Ultra™ II FS DNA Library Prep Kit
- Improving NGS library performance with lower input amounts using the NEBNext Ultra II RNA Library Prep Kit for Illumina (non-directional) – Advances in non-strand-specific RNA library construction in a ribosomal RNA depletion workflow
- Improving NGS library performance with lower input amounts using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (non-directional)
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Improving Enzymatic DNA Fragmentation for Next Generation Sequencing Library Construction
Among the available options for DNA fragmentation, enzymatic fragmentation is demonstrably gentler on the sample, yielding less damage at any scale. The new NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® includes a single step for enzymatic fragmentation, end repair, and dA tailing.
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Advances in next generation sequencing: How researchers at NEB are working to improve our understanding of the genome, epigenome and transcriptome.
NEB scientists develop sequencing technologies to analyze genome-wide methylation, transcriptional start sites, full-length transcriptomes, chromatin accessibility, and much more.
- Sequencing to Insight: Real-world applications powered by NEBNext® Poster
- NEBNext Ultra II Reagents & Kits Brochure
- NEBNext For RNA Sample Prep Brochure
- NEBNext RNA Depletion Brochure
- NEBNext Direct Genotyping Solution Brochure
- NEBNext® for DNA Sample Prep Brochure
- NEBNext for Ion Torrent™ Brochure
- NEB E3406 LV UDI IlluminaLRM
- Induro™, a Novel Reverse Transcriptase for Nanopore Direct RNA Sequencing with Significantly Improved RNA 5′ Coverage
- Streamlined, Express DNA Library Preparation Methods Meet Requirements of High-throughput Library Construction
- NEBNext UltraExpress™ RNA: A fast and flexible workflow for stranded RNA-seq library preparation
- NEBNext® solutions for challenging methods and samples
- E5hmC-seq™: Detection of 5hmC at single base resolution
- E5hmC-seq: Detection of 5hmC at single-base resolution
- Streamlining RNA and DNA library construction methods to meet the challenges of high-throughput sequencing
- Matched fresh frozen and FFPE patient tissues reveal the enhanced sensitivity and data quality of a novel DNA library prep method
- Streamlined whole-genome and targeted sequencing workflows for prevalent RNA viruses: SARS-CoV-2, Respiratory Syncytial Virus, and Influenza A
- Methods for sequencing cell-free DNA and cell-free RNA from human plasma
- An end-to-end enzymatic solution for methylomes from low-input DNA
- Germline variant calling with EM-seq data
- A sample-to-results workflow for Respiratory Syncytial Virus (RSV) whole-genome sequencing
- Accurate detection of small non-coding RNAs using NEBNext® Low-bias Small RNA Library Prep Kit
- Integration of whole exome sequencing and RNA-seq facilitates somatic mutation detection in tumor tissue samples
- High-throughput methyl-binding domain-based DNA enrichment and sequencing
- Real-world applications powered by NEBNext® Poster
- Rapid, robust and sensitive DNA, RNA and small RNA library construction methods for evaluation of various samples and species
Feature Articles
Brochures
Usage Guidelines
Posters
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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