NEBuilder® HiFi DNA Assembly Master Mix

NEBuilder HiFi DNA Assembly Master Mix (NEB #M5520) has been reformulated with components containing Recombinant Albumin (rAlbumin) beginning with Lot #10238675.

Did you know this product can be purchased in larger volumes? Submit an inquiry to find out more.

NEBuilder® HiFi DNA Assembly Master Mix allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format. Visit for more information.

  • Also available with chemically competent cells at a discounted price.
  • Enjoy less screening/re-sequencing of constructs, with virtually error-free, high-fidelity assembly
  • Flexible sequence design (scar-less cloning)
  • No PCR clean-up step required
  • Join DNA fragments together more efficiently, even with larger fragments or low DNA inputs
  • Use NEBuilder HiFi in successive rounds of assembly, because it removes 5´ and 3´ end mismatches. (Save time by avoiding time-consuming PCR amplification steps.)
  • Bridge two ds-fragments with a synthetic ss-DNA oligo for simple and fast construction (e.g., linker insertion or gRNA library)
  • Use with lower DNA input requirements
  • Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
  • No licensing fee requirements from NEB for NEBuilder products
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Catalog # Concentration Size List Price Your Price Quantity
E2621S Not Applicable 10 reactions $173.00
E2621L Not Applicable 50 reactions $683.00
E2621X Not Applicable 250 reactions $2,680.00
Please enter a quantity for at least one size

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