Features

• Large plasmid and BAC cloning
• DH10B™ derivative

Application Features

Effect of Plasmid Size on Transformation Efficiency: 

Effect of heat shock time on NEB 10-beta competent E. coli transformation efficiency:   50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.

Effect of DNA incubation time on NEB 10-beta competent E. coli transformation efficiency: 

Effect of outgrowth medium on transformation efficiency:

Benefit from the high transformation efficiencies of NEB 10-beta:

 

Antibiotics for Plasmid Selection	Working Concentration
Ampicillin	100 μg/ml
Carbenicillin	100 μg/ml
Chloramphenicol	33 μg/ml
Kanamycin	30 μg/ml
Tetracycline	15 μg/ml

Storage Temperature

-80°C

Shipping Notes

• Ships on dry ice

Antibiotic Resistance

• str

Materials Sold Separately

• NEB® 10-beta/Stable Outgrowth Medium

1. CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
2. STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.

1. Which competent cell strains are compatible with Gateway® Cloning?  Which strains are not compatible?
2. Do you carry competent cells which are compatible with Gateway® Cloning?
3. What are the strain properties (C3019)?
4. What is the difference between NEB #C3019H and NEB #C3019I?
5. What is the shelf life for this strain (NEB #C3019H and NEB #C3019I)?
6. Which strain of Competent E. coli should I use for general cloning?
7. Does plasmid size affect transformation efficiency (C3019)?
8. How should I calculate the transformation efficiency (C3019)?
9. Can I store competent cells at -20°C instead of -80°C?
10. What is the optimal heat shock time for this strain (NEB #C3019H and NEB #C3019I)?
11. How long should I incubate cells on ice after DNA has been added (NEB #C3019H and NEB #C3019I)?
12. Which kind of transformation tubes should be used?
13. What volume of DNA can be added into competent cells?
14. Are NEB's competent cells compatible with the “Mix & Go" protocol?
15. What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix?
16. Why did Synthetic Biologist Chris Voigt of MIT choose NEB 10-beta for DNA assembly and cloning?
17. How should I store the NEB 10-beta/Stable Outgrowth Medium?

1. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (High Efficiency) (C3019)
2. 5 Minute Transformation Protocol using NEB® 10-beta Competent E. coli (C3019)

• Characteristics of Select E.coli Strains
• Cloning Competent Cell Transformation Selection Chart
• Competent Cell Product Comparison
• Competent Cell Selection Guide
• Strain Properties

• Additional E. coli Strain Genotypes
• Chemical Transformation Tips
• Convenient Formats of Competent Cells
• Genetic Markers
• McrA, McrBC and EcoKI Strain Phenotypes
• Restriction of Foreign DNA by E. coli K-12

• NEBcloner
• NEBioCalculator

• Enhancing Transformation Efficiency C3019

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• C3019H_I_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• C3019H_I_v1_3031607
• C3019H_I_v1_3001607
• C3019H_I_v1_2881608
• C3019H_I_v1_2971607
• C3019H_I_v1_3001608
• C3019H_I_v1_3041608
• C3019H_I_v1_3051608
• C3019H_I_v1_2991608
• C3019H_I_v1_2991609
• C3019H_I_v1_3061608
• C3019H_I_v1_3071609
• C3019H_I_v1_3081609
• C3019H_I_v1_3061610
• C3019H_I_v1_3011609
• C3019H_I_v1_3011611
• C3019H_I_v1_3071611
• C3019H_I_v1_3091610
• C3019H_I_v1_3091611
• C3019H_I_v1_3051610
• C3019H_I_v1_3111611
• C3019H_I_v1_3121611
• C3019H_I_v1_2951604
• C3019H_I_v1_3131612
• C3019H_I_v1_3031612
• C3019H_I_v1_3111701
• C3019H_I_v1_3151701
• C3019H_I_v1_3161701
• C3019H_I_v1_3101610
• C3019H_I_v1_3171701
• C3019H_I_v1_3101701
• C3019H_I_v1_3091701
• C3019H_I_v1_3021703
• C3019H_I_v1_3161703
• C3019H_I_v1_3041704
• C3019H_I_v1_3191705
• C3019H_I_v1_3211705
• C3019H_I_v1_3221705
• C3019H_I_v1_3131705
• C3019H_I_v1_3171706
• C3019H_I_v1_3181706
• C3019H_I_v1_3191708
• C3019H_I_v1_3211708
• C3019H_I_v1_3221708
• C3019H_I_v1_3231707
• C3019H_I_v1_3241707
• C3019H_I_v1_3251706
• C3019H_I_v1_3251710
• C3019H_I_v1_3261710
• C3019H_I_v1_3271707
• C3019H_I_v1_3271708
• C3019H_I_v1_3271710
• C3019H_I_v1_3281708
• C3019H_I_v1_3291708
• C3019H_I_v1_3301708
• C3019H_I_v1_3311708
• C3019H_I_v1_3311711
• C3019H_I_v1_3341710
• C3019H_I_v1_3351711
• C3019H_I_v1_3361711
• C3019H_I_v1_3291710
• C3019H_I_v1_3361712
• C3019H_I_v1_3281712
• C3019H_I_v1_3291711
• C3019H_I_v1_3291712
• C3019H_I_v1_3391712
• C3019H_I_v1_3331801
• C3019H_I_v1_3401712
• C3019H_I_v1_3301801
• C3019H_I_v1_3321802
• C3019H_I_v1_3331802
• C3019H_I_v1_3411802
• C3019H_I_v1_3421802
• C3019H_I_v1_3431802
• C3019H_I_v1_3381803
• C3019H_I_v1_3391803
• C3019H_I_v1_3461803

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEB® 10-beta Competent E. coli (High Efficiency)
• NEB^® 10-beta/Stable Outgrowth Medium
• pUC19 Vector

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Trademarks

ONESHOT^® and MAX EFFICIENCY^® are registered trademarks of Thermo Fisher Scientific.
DH10B™ is a trademarks of Thermo Fisher Scientific.