Home FAQs I would like to assemble ssDNA oligonucleotides into dsDNA fragments. Do I need to use oligonucleotides that have been purified by PAGE or HPLC?

FAQ: I would like to assemble ssDNA oligonucleotides into dsDNA fragments. Do I need to use oligonucleotides that have been purified by PAGE or HPLC?

No. Standard, desalted primers may be used.

For more information, please download the application note, Construction of an sgRNA-Cas9 expression vector via single-stranded DNA oligo bridging of double-stranded DNA fragments.

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NEBUILDER^® HIFI DNA ASSEMBLY^® : Bridging dsDNA with a ssDNA Oligo

Learn how NEBuilder® HiFi DNA Assembly bridges dsDNA with a ssDNA oligo.

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NEBUILDER® HIFI DNA ASSEMBLY® : Bridging dsDNA with a ssDNA Oligo

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NEBUILDER^® HIFI DNA ASSEMBLY^® : Bridging dsDNA with a ssDNA Oligo