NEB® 10-beta Electrocompetent E. coli
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
- High efficiency strain ideal for cloning large plasmids and BACs
- Available in chemically-competent format (single-use and 200 μl vials)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
Ordering Information

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Product Information
NEB 10-beta electrocompetent E. coli cells are optimized for high efficiency transformation by electroporation. These cells are ideal for DNA library constructions and all cloning purposes.
Highlights
- DH10B™ derivative
- Transformation efficiency: 2 x 1010 cfu/μg pUC19
- Accomodation of large plasmids including BAC and cosmid constructs
- Efficient transformation of methylated DNA derived from eukaryotic sources and unmethylated DNA derived from PCR, cDNA and other sources
- Activity of nonspecific endonuclease I eliminated for highest quality plasmid preparations (endA1)
- Suitable for blue/white screening by α-complementation of the β-galactosidase gene (Φ80ΔlacZM15)
- Reduced recombination of cloned DNA (recA)
- Resistance to phage T1 lytic (fhuA2)
- K-12 Strain
- Free of animal products
Genotype
Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- Φ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration NEB® 10-beta/Stable Outgrowth Medium 4 1 X pUC19 Vector -20 0.05 ng/μl - Product Categories:
- Cloning Competent Cell Strains Products
- Applications:
- Transformation,
- E Coli Protein Expression
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