NEBuilder HiFi DNA Assembly offers several advantages over NEB Gibson Assembly. NEBuilder HiFi utilizes a proprietary higher fidelity polymerase, which results in less screening/re-sequencing of constructs and virtually error-free, high-fidelity assembly. This feature also enables NEBuilder to be used in certain applications that NEB Gibson Assembly cannot be used for:
- NEBuilder HiFi removes 5´- and 3´-end mismatches, and can be used in successive rounds of assembly. This saves time by avoiding time-consuming PCR amplification steps.
- NEBuilder HiFi can bridge two ds-fragments with a synthetic ssDNA oligo for simple and fast construction (e.g., linker insertion or gRNA library).
For more detailed information and data, please visit NEBuilderHifi.com
