Home FAQs Is it necessary to inactivate restriction enzymes after vector digestion?

FAQ: Is it necessary to inactivate restriction enzymes after vector digestion?

Inactivation of restriction endonucleases is generally not necessary, but in some cases it might increase the transformation efficiency. If the insert and final assembled product also carry the restriction site that was used to linearize the vector it is necessary to heat inactivate the restriction enzyme or purify the cut vector if heat inactivation is not possible.