Q5® High-Fidelity DNA Polymerase
Fidelity at its finest – for over 10 years
This product is available in a glycerol-free format. Contact us for more information.
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Also available: Q5 Hot Start standalone polymerase (NEB #M0493) and master mix formats (NEB #M0492, M0494)
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Product Information
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity DNA Polymerase. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis. 
Product Source
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.- This product is related to the following categories:
- Q5® High-Fidelity DNA Polymerases Products
- This product can be used in the following applications:
- Gibson Assembly®,
- Long Range PCR,
- Fast PCR,
- High-Fidelity PCR,
- Multiplex PCR,
- Specialty PCR,
- DNA Amplification, PCR & qPCR,
- Routine PCR,
- PCR, Fast Cloning: Accelerate your cloning workflows with reagents from NEB
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Additional Citations
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- Yonghe Zhang, Huiming Huang, Shanshan Xu, Bo Wang, Jianhua Ju, Huarong Tan, Wenli Li (2015) Activation and enhancement of Fredericamycin A production in deepsea-derived Streptomyces somaliensis SCSIO ZH66 by using ribosome engineering and response surface methodology. Microb Cell Fact; 14, 64. PubMedID: 25927229, DOI: 10.1186/s12934-015-0244-2
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- Longhai Dai, Can Liu, Yueming Zhu, Jiangsheng Zhang, Yan Men, Zeng Yan, Yuanxia Sun (2015) Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii. Plant Cell Physiol; , PubMedID: 25759326, DOI: 10.1093/pcp/pcv043
- Yang YJ, Han YY, Chen K, Zhang Y, Liu X, Li S, Wang KQ, Ge JB, Liu W, Zuo J. (2015) TonEBP modulates the protective effect of taurine in ischemia-induced cytotoxicity in cardiomyocytes Cell Death Dis; PubMedID: 26673669, DOI: 10.1038
- Yuan Xue, Jossef Osborn, Anand Panchal, Jay L Mellies (2015) The RpoE Stress Response Pathway Mediates Reduction of the Virulence of Enteropathogenic Escherichia coli by Zinc. Appl Environ Microbiol; 81, 3766-74. PubMedID: 25819956, DOI: 10.1128/AEM.00507-15
- Christine Henke, Pamela L Strissel, Maria-Theresa Schubert, Megan Mitchell, Claus C Stolt, Florian Faschingbauer, Matthias W Beckmann, Reiner Strick (2015) Selective expression of sense and antisense transcripts of the sushi-ichi-related retrotransposon - derived family during mouse placentogenesis. Retrovirology; 12, 9. PubMedID: 25888968, DOI: 10.1186/s12977-015-0138-8
- Binyamin D Berkovits, Christine Mayr (2015) Alternative 3' UTRs act as scaffolds to regulate membrane protein localization. Nature; , PubMedID: 25896326, DOI: 10.1038/nature14321
- Wang XJ, Zhang XJ, Hu W, Zhang TY, Wang SQ (2014) A simple and efficient strategy for the de novo construction of greater-than-genome-length hepatitis B virus replicons J Virol Methods; 207, 158-62. PubMedID: 25025817, DOI: 10.1016/j.jviromet.2014.07.009
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- Martin Kostovcik, Craig C Bateman, Miroslav Kolarik, Lukasz L Stelinski, Bjarte H Jordal, Jiri Hulcr (2014) The ambrosia symbiosis is specific in some species and promiscuous in others: evidence from community pyrosequencing. ISME J; , PubMedID: 25083930, DOI: 10.1038/ismej.2014.115
- Bert De Rybel, Milad Adibi, Alice S. Breda, Jos R. Wendrich, Margot E. Smit, Ondej Novk, Nobutoshi Yamaguchi, Saiko Yoshida, Gert Van Isterdael, Joakim Palovaara, Bart Nijsse, Mark V. Boekschoten, Guido Hooiveld, Tom Beeckman, Doris Wagner, Karin Ljung, Christian Fleck, Dolf Weijers (2014) Integration of growth and patterning during vascular tissue formation in Arabidopsis Science; 345, 1255215. PubMedID: 25104393, DOI: 10.1126/science.1255215
- Xin Duan, Arjun Krishnaswamy, Irina De la Huerta, Joshua R Sanes (2014) Type II Cadherins Guide Assembly of a Direction-Selective Retinal Circuit. Cell; 158, 793-807. PubMedID: 25126785, DOI: 10.1016/j.cell.2014.06.047
- Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013) ROLE OF HUMAN HYPOXANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR) Mol Pharmacol; 87247. PubMedID: 23907213
- Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc (2013) Hedgehog Signaling Regulates MyoD Expression and Activity J Biol Chem; 288(6), 4389–4404. PubMedID: 23266826
- Hicham Bouabe, Klaus Okkenhaug (2013) A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells Methods Mol Biol; 1064, 337-354. PubMedID: 23996269
- Wilber Quispe-Tintaya, Ryan R White, Vasily N Popov, Jan Vijg, Alexander Y Maslov (2013) Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing Biotechniques; 55(3), 133-6. PubMedID: 24003945, DOI: 10.2144/000114077
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Important Tips for Q5® Polymerase
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Why Choose Q5® High-fidelity Polymerase?
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Behind the paper: Examining Sources of Error in PCR by Single-Molecule Sequencing
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Why is Tm Important in Primer Design?
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Tips for Amplifying Large Amplicons
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How to Amplify GC-rich DNA
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5 Tips for Setting Up Your PCR
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Overview of PCR
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