Home FAQs Is this method applicable to the assembly of repetitive sequences?

FAQ: Is this method applicable to the assembly of repetitive sequences?

Yes. However, one must ensure that each DNA fragment includes a unique overlap so that the sequences may anneal and are properly arranged. The repetitive sequence can also be internalized in the first stage of a two-stage assembly strategy. If having repetitive sequences at the ends of each fragment is unavoidable, the correct DNA assembly may be produced, albeit at lower efficiency than other, unintended assemblies. Alternatively, consider Golden Gate Assembly for assemblies containing repetitive sequences.

Videos

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    NEBUILDER® Assembly Tool 2.0 What’s New?

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