Gibson Assembly® Master Mix

Gibson Assembly Master Mix (NEB #M5510) has been reformulated with components containing Recombinant Albumin (rAlbumin) beginning with Lot #10229799. Learn more.

Have you tried NEBuilder HiFi DNA Assembly? NEBuilder HiFi offers several advantages over NEB Gibson Assembly. For more information, visit

Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.

  • Assembly and transformation in just under two hours
  • Flexible sequence design (scar-less cloning)
  • No PCR clean-up step required
  • High transformation efficiencies for inserts up to 20 kb
  • Easily adapted for multiple DNA manipulations, including site-directed mutagenesis

We're sorry, this butterfly has already been found. Learn more about how NEB is celebrating our 50th anniversary.

Catalog # Concentration Size List Price Your Price Quantity
E2611S Not Applicable 10 reactions $180.00
E2611L Not Applicable 50 reactions $711.00
Please enter a quantity for at least one size
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