Q5® High-Fidelity 2X Master Mix
Fidelity at its finest – for over 10 years
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
- Highest fidelity amplification (~280X higher than Taq)
- Master mix includes Q5 High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a broad-use buffer
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Also available: Q5 Hot Start standalone polymerase (NEB #M0493) and hot start master mix formats (NEB #M0494)
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Product Information
The Q5 High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format. The Q5 High- Fidelity 2X Master Mix features a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM Mg++. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Q5-High Fidelity 2X Master Mix formats allow robust amplification of a broad range of targets with a single formulation.
Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity 2X Master Mix. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis.
Product Source
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.- This product is related to the following categories:
- Q5® High-Fidelity DNA Polymerases Products,
- Master Mixes Products
- This product can be used in the following applications:
- Long Range PCR,
- Fast PCR,
- High-Fidelity PCR,
- Multiplex PCR,
- Specialty PCR,
- DNA Amplification, PCR & qPCR,
- Routine PCR,
- PCR, Fast Cloning: Accelerate your cloning workflows with reagents from NEB
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Powered by BiozFeatured Videos
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Important Tips for Q5® Polymerase
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Why Choose Q5® High-fidelity Polymerase?
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Behind the paper: Examining Sources of Error in PCR by Single-Molecule Sequencing
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Why is Tm Important in Primer Design?
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Tips for Amplifying Large Amplicons
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How to Amplify GC-rich DNA
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5 Tips for Setting Up Your PCR
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Overview of PCR
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Quick Tips - What do I do if my master mix has a precipitate?
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