HomeFAQsCan I use a 15-nt overlap that is entirely composed of His-tag repeats (i.e. CACCACCACCACCAC)?
FAQ: Can I use a 15-nt overlap that is entirely composed of His-tag repeats (i.e. CACCACCACCACCAC)?
No, after the His-tag, you must include at least 3 nucleotides, that are not part
of the His-tag repeating sequence. Avoid repeating sequences at the end of an
overlap.
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