Q5® Hot Start High-Fidelity 2X Master Mix
Featured in the ARTIC SARS-CoV-2 sequencing workflow (1) and a component in the NEBNext® ARTIC SARS-CoV-2 kits.
Fidelity at its finest – for over 10 years
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
- Highest fidelity amplification (~280X higher than Taq)
- Master mix includes Q5 Hot Start High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a broad-use buffer
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Bulk format available: M0494X = 1 x 12.5 ml bottle
1. Josh Quick 2020. nCoV-2019 sequencing protocol v3 (LoCost). protocols.io https://www.protocols.io/view/ncov-2019-sequencing-protocol-v3-locost-bh42j8ye
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Product Information
The Q5® Hot Start High-Fidelity 2X Master Mix features a high-fidelity, thermostable, hot start DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity 2X Master Mix is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 Hot Start High-Fidelity Master Mix contains 2 mM Mg++. Q5 Hot Start High-Fidelity 2X Master Mix is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Q5-High Fidelity 2X Master Mix formats allow robust amplification of a broad range of targets with a single formulation.
Amplification of a variety of human genomic amplicons from low to high GC content using Q5 Hot Start High-Fidelity 2X Master Mix. All reactions were set up at room temperature, conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis. 
Product Source
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.- This product is related to the following categories:
- Q5® High-Fidelity DNA Polymerases Products,
- Master Mixes Products
- This product can be used in the following applications:
- Site Directed Mutagenesis,
- Site Directed Mutagenesis,
- High-throughput cloning and automation solutions,
- Hot Start PCR,
- Long Range PCR,
- Fast PCR,
- High-Fidelity PCR,
- Multiplex PCR,
- Specialty PCR,
- DNA Amplification, PCR & qPCR,
- Routine PCR,
- PCR, Fast Cloning: Accelerate your cloning workflows with reagents from NEB
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Powered by BiozFeatured Videos
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Important Tips for Q5® Polymerase
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Why Choose Q5® High-fidelity Polymerase?
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Behind the paper: Examining Sources of Error in PCR by Single-Molecule Sequencing
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Why is Tm Important in Primer Design?
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Tips for Amplifying Large Amplicons
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How to Amplify GC-rich DNA
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5 Tips for Setting Up Your PCR
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Overview of PCR
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Quick Tips - What do I do if my master mix has a precipitate?
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