Fidelity at its finest – for over 10 years
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Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
Q5® High-Fidelity DNA Polymerase
is a high-fidelity, thermostable DNA polymerase
with 3´→ 5´ exonuclease activity, fused to a
processivity-enhancing Sso7d domain to support
robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity
DNA Polymerase is ideal for cloning and can
be used for long or difficult amplicons. Q5
High-Fidelity DNA Polymerase is supplied with
an optimized buffer system that allows robust
amplification regardless of GC content. The 5X
Q5 Reaction Buffer contains 2 mM Mg++ at final
(1X) reaction concentrations and is recommended
for most routine applications. For GC-rich targets
(≥ 65% GC), amplification can be improved by
the addition of the 5X Q5 High GC Enhancer. Q5
High-Fidelity DNA Polymerase is unlike typical,
lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
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