Fidelity at its finest – for over 10 years
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
The Q5 High-Fidelity 2X Master
Mix offers robust, high-fidelity performance in a
convenient master mix format. The Q5 High-
Fidelity 2X Master Mix features a high-fidelity,
thermostable DNA polymerase with 3´→ 5´
exonuclease activity, fused to a processivity-enhancing
Sso7d domain to support robust DNA
amplification. With an error rate ~280-fold lower
than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase
is ideal for cloning and can be used for long or
difficult amplicons. The convenient master mix
formulation is supplied at a 2X concentration.
The mix contains dNTPs, Mg++ and a proprietary
broad-use buffer requiring only the addition of
primers and DNA template for robust amplification
regardless of GC content. When used at the
recommended 1X final concentration, the Q5
High-Fidelity Master Mix contains 2 mM Mg++.
Q5 High-Fidelity DNA Polymerase is unlike typical,
lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
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