Add SOC to freshly transformed E. coli cells and incubate for up to 1 hour before plating on selective media.
1X SOC Outgrowth Medium:
2% Vegetable Peptone
0.5% Yeast Extract
10 mM NaCl
2.5 mM KCl
10 mM MgCl2
10 mM MgSO4
20 mM Glucose
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
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