neb31 cloned at NEB recombinant engineered time saver dil_B 37 80 Heat CpG dcm

BsaI-HFv2 has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10156743. Learn more.

Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments).

BsaI-HFv2 is NEB Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNNEB cutting by BsaI Works well for any protocol requiring DNNEB cutting by BsaI Recommended enzyme for any purpose requiring digestion at the recognition sequence 5-GGTCTC(N1)/(N5)-3
High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart™ Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

  • Engineered for improved performance
  • Optimized for Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer often used in this application
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • 100% activity in rCutSmart Buffer
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • Reduced star activity
  • NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly.
  • Can be used with NEBridge® Ligase Master Mix for Golden Gate Assembly
  • Restriction Enzyme Cut Site: GGTCTC(1/5)

"I really, really love BsaI-HFv2. We are back to getting phenomenal GGA results again with very little background."

    -M.C, Principal Investigator, Davidson College, NC

Catalog # Concentration Size List Price Your Price Quantity
R3733S 20,000 units/ml 1,000 units $78.00
R3733L 20,000 units/ml 5,000 units $323.00
Please enter a quantity for at least one size

Need a custom/large volume order? Contact us
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