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Restriction Endonucleases

Cut Smarter with Restriction Enzymes from NEB

With over 45 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

Convenience

A vial of 6X Purple Load Dye is included with most restriction enzymes.
Over 210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer.
>180 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight.
Choose from >265 restriction enzymes, the largest selection commercially available.

Performance

Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in rCutSmart Buffer. A vial of 6X Purple Load Dye is included with every HF restriction enzyme.
All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.

Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.

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Restriction Endonucleases

Restriction Endonucleases: A

Restriction Endonucleases: B

Restriction Endonucleases: C-G

Restriction Endonucleases: H-M

Restriction Endonucleases: N-O

Restriction Endonucleases: P-R

Restriction Endonucleases: S

Restriction Endonucleases: T-Z

HF®, Nicking, Master Mix, Time-Saver & Other

Restriction Endonuclease Buffers & Diluents

Restriction Endonucleases: A

Restriction Endonucleases: B

Restriction Endonucleases: C-G

Restriction Endonucleases: H-M

Restriction Endonucleases: N-O

Restriction Endonucleases: P-R

Restriction Endonucleases: S

Restriction Endonucleases: T-Z

HF®, Nicking, Master Mix, Time-Saver & Other

High-Fidelity (HF®) Restriction Endonucleases

Homing Endonucleases

Nicking Endonucleases

Restriction Enzymes for Epigenetic Analysis

Time-Saver™ Qualified Restriction Enzymes

Restriction Endonuclease Buffers & Diluents

Videos

What is a Type II Restriction Enzyme?

Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work.
What is a Type I Restriction Enzyme?

Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Learn more about how Type I REs work.
What is a Type III Restriction Enzyme?

Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. Learn more about these poorly understood enzymes.
TIME-SAVER™ Qualified Restriction Enzymes

How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.
Cloning With Restriction Enzymes

Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.
Reduce Star Activity with High-Fidelity Restriction Enzymes

NEB has engineered HF® enzymes to eliminate star activity. Learn how, and what this means for your digests.
Standard Protocol for Restriction Enzyme Digests

Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.
What are Nicking Enzymes and How are They Used?

Learn more about nicking endonucleases (aka nicking enzymes), how they work and their role in different applications including virus detection and SDA.
What are Restriction Enzymes?

Watch as Geoff Wilson, Emeritus Scientist, describes what restriction enzymes are and how they revolutionized molecular biology.
Why is My Restriction Enzyme Not Cutting DNA?

Not getting the cleavage you expected? Let an NEB scientist help you troubleshoot your reaction.
Restriction Enzyme Digest Problem: Too Many DNA Bands

Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause.
Restriction Enzyme Digest Protocol: Cutting Close to DNA End

When cutting close to the end of a DNA molecule, make sure you know how many bases to add to the ends of your PCR primers.
The Effect of DNA Methylation on Restriction Digests

This tutorial explains the effect of DNA methylation on restriction enzyme digestion; including dam, dcm and CpG methylation.
Restriction Enzymes in Isothermal Amplification

Isothermal amplification generates many copies of a target sequence in a short period of time, at a constant temperature. Learn more about isothermal amplification.