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Restriction Endonucleases Products

Cut Smarter with Restriction Enzymes from NEB

With over 45 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

Convenience

  • A vial of 6X Purple Load Dye is included with most restriction enzymes.
  • Over 210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer. 
  • >180 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight.
  • Choose from >265 restriction enzymes, the largest selection commercially available.



Performance

  • Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in rCutSmart Buffer. A vial of 6X Purple Load Dye is included with every HF restriction enzyme.
  • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.


Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.


Choose Product:

Restriction Endonucleases
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WarmStart® Nt.BstNBI

Restriction Endonucleases: A Products

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AatII

Close

Acc65I

Close

AccI

Close

AciI

Close

AclI

Close

AcuI

Close

AfeI

Close

AflII

Close

AflIII

Close

AgeI-HF®

Close

AhdI

Close

AleI-v2

Close

AluI

Close

AlwI

Close

AlwNI

Close

ApaI

Close

ApaLI

Close

ApeKI

Close

ApoI-HF®

Close

AscI

Close

AseI

Close

AsiSI

Close

AvaI

Close

AvaII

Close

AvrII

Restriction Endonucleases: B Products

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BaeGI

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BaeI

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BamHI

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BamHI-HF®

Close

BanI

Close

BanII

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BbsI

Close

BbsI-HF®

Close

BbvCI

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BbvI

Close

BccI

Close

BceAI

Close

BcgI

Close

BciVI

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BclI

Close

BclI-HF

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BcoDI

Close

BfaI

Close

BfuAI

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BglI

Close

BglII

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BlpI

Close

BmgBI

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BmrI

Close

BmtI-HF®

Close

BpmI

Close

Bpu10I

Close

BpuEI

Close

BsaAI

Close

BsaBI

Close

BsaHI

Close

BsaI-HF®v2

Close

BsaJI

Close

BsaWI

Close

BsaXI

Close

BseRI

Close

BseYI

Close

BsgI

Close

BsiEI

Close

BsiHKAI

Close

BsiWI

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BsiWI-HF®

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BslI

Close

BsmAI

Close

BsmBI-v2

Close

BsmFI

Close

BsmI

Close

BsoBI

Close

Bsp1286I

Close

BspCNI

Close

BspDI

Close

BspEI

Close

BspHI

Close

BspMI

Close

BspQI

Close

BsrBI

Close

BsrDI

Close

BsrFI-v2

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BsrGI-HF®

Close

BsrI

Close

BssHII

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BssSI-v2

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BstAPI

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BstBI

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BstEII-HF®

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BstNI

Close

BstUI

Close

BstXI

Close

BstYI

Close

BstZ17I-HF®

Close

Bsu36I

Close

BtgI

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BtgZI

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BtsCI

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BtsIMutI

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BtsI-v2

Restriction Endonucleases: C-G Products

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Cac8I

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ClaI

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CspCI

Close

CviKI-1

Close

CviQI

Close

DdeI

Close

DpnI

Close

DpnII

Close

DraI

Close

DraIII-HF®

Close

DrdI

Close

EaeI

Close

EagI-HF®

Close

EarI

Close

EciI

Close

Eco53kI

Close

EcoNI

Close

EcoO109I

Close

EcoP15I

Close

EcoRI

Close

EcoRI-HF®

Close

EcoRV

Close

EcoRV-HF®

Close

Esp3I

Close

FatI

Close

FauI

Close

Fnu4HI

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FokI

Close

FseI

Close

FspI

Restriction Endonucleases: H-M Products

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HaeII

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HaeIII

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HgaI

Close

HhaI

Close

HincII

Close

HindIII

Close

HindIII-HF®

Close

HinfI

Close

HinP1I

Close

HpaI

Close

HpaII

Close

HphI

Close

Hpy166II

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Hpy188I

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Hpy188III

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Hpy99I

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HpyAV

Close

HpyCH4III

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HpyCH4IV

Close

HpyCH4V

Close

I-CeuI

Close

I-SceI

Close

KasI

Close

KpnI-HF®

Close

MboI

Close

MboII

Close

MfeI-HF®

Close

MluCI

Close

MluI-HF®

Close

MlyI

Close

MmeI

Close

MnlI

Close

MscI

Close

MseI

Close

MslI

Close

MspA1I

Close

MspI

Close

MspJI

Close

MwoI

Restriction Endonucleases: N-O Products

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NaeI

Close

NarI

Close

Nb.BbvCI

Close

Nb.BsmI

Close

Nb.BsrDI

Close

Nb.BssSI

Close

Nb.BtsI

Close

NciI

Close

NcoI

Close

NcoI-HF®

Close

NdeI

Close

NgoMIV

Close

NheI-HF®

Close

NlaIII

Close

NlaIV

Close

NmeAIII

Close

NotI

Close

NotI-HF®

Close

NruI-HF®

Close

NsiI

Close

NsiI-HF®

Close

NspI

Close

Nt.AlwI

Close

Nt.BbvCI

Close

Nt.BsmAI

Close

Nt.BspQI

Close

Nt.BstNBI

Close

Nt.CviPII

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WarmStart® Nt.BstNBI

Restriction Endonucleases: P-R Products

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PacI

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PaeR7I

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PaqCI®

Close

PciI

Close

PflFI

Close

PflMI

Close

PI-PspI

Close

PI-SceI

Close

PleI

Close

PluTI

Close

PmeI

Close

PmlI

Close

PpuMI

Close

PshAI

Close

PsiI-v2

Close

PspGI

Close

PspOMI

Close

PspXI

Close

PstI

Close

PstI-HF®

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PvuI-HF®

Close

PvuII

Close

PvuII-HF®

Close

RsaI

Close

RsrII

Restriction Endonucleases: S Products

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SacI-HF®

Close

SacII

Close

SalI

Close

SalI-HF®

Close

SapI

Close

Sau3AI

Close

Sau96I

Close

SbfI-HF®

Close

ScaI-HF®

Close

ScrFI

Close

SexAI

Close

SfaNI

Close

SfcI

Close

SfiI

Close

SfoI

Close

SgrAI

Close

SmaI

Close

SmlI

Close

SnaBI

Close

SpeI-HF®

Close

SphI

Close

SphI-HF®

Close

SrfI

Close

SspI-HF®

Close

StuI

Close

StyD4I

Close

StyI-HF®

Close

SwaI

Restriction Endonucleases: T-Z Products

Close

TaqI-v2

Close

TfiI

Close

TseI

Close

Tsp45I

Close

TspMI

Close

TspRI

Close

Tth111I

Close

XbaI

Close

XcmI

Close

XhoI

Close

XmaI

Close

XmnI

Close

ZraI

HF®, Nicking, Master Mix, Time-Saver & Other Products

Close

AatII

Close

Acc65I

Close

AccI

Close

AciI

Close

AclI

Close

AcuI

Close

AflII

Close

AgeI-HF®

Close

AhdI

Close

AluI

Close

AlwNI

Close

ApaI

Close

ApaLI

Close

ApeKI

Close

ApoI-HF®

Close

AscI

Close

AseI

Close

AvaI

Close

AvaII

Close

AvrII

Close

BaeGI

Close

BaeI

Close

BamHI

Close

BamHI-HF®

Close

BbsI

Close

BbsI-HF®

Close

BbvI

Close

BciVI

Close

BclI

Close

BclI-HF

Close

BcoDI

Close

BfuAI

Close

BglI

Close

BglII

Close

BlpI

Close

BmgBI

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BmtI-HF®

Close

BpmI

Close

BpuEI

Close

BsaAI

Close

BsaHI

Close

BsaI-HF®v2

Close

BsaWI

Close

BsaXI

Close

BseRI

Close

BsgI

Close

BsiEI

Close

BsiWI

Close

BsiWI-HF®

Close

BslI

Close

BsmAI

Close

BsmBI-v2

Close

BsmI

Close

BsoBI

Close

Bsp1286I

Close

BspCNI

Close

BspEI

Close

BspHI

Close

BspQI

Close

BsrBI

Close

BsrDI

Close

BsrGI-HF®

Close

BsrI

Close

BssHII

Close

BstBI

Close

BstEII-HF®

Close

BstNI

Close

BstUI

Close

BstXI

Close

BstYI

Close

BstZ17I-HF®

Close

Bsu36I

Close

BtgI

Close

BtsCI

Close

Cac8I

Close

ClaI

Close

CspCI

Close

CviQI

Close

DdeI

Close

DpnI

Close

DpnII

Close

DraI

Close

DraIII-HF®

Close

DrdI

Close

EagI-HF®

Close

EarI

Close

Eco53kI

Close

EcoNI

Close

EcoO109I

Close

EcoP15I

Close

EcoRI

Close

EcoRI-HF®

Close

EcoRV

Close

EcoRV-HF®

Close

Esp3I

Close

Fnu4HI

Close

FseI

Close

FspI

Close

HaeIII

Close

HhaI

Close

HincII

Close

HindIII-HF®

Close

HinfI

Close

HinP1I

Close

HpaII

Close

HphI

Close

Hpy166II

Close

HpyAV

Close

HpyCH4IV

Close

HpyCH4V

Close

I-CeuI

Close

I-SceI

Close

KpnI-HF®

Close

MboI

Close

MfeI-HF®

Close

MluCI

Close

MluI-HF®

Close

MlyI

Close

MmeI

Close

MnlI

Close

MseI

Close

MslI

Close

MspA1I

Close

MspI

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MspJI

Close

MwoI

Close

Nb.BbvCI

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Nb.BsmI

Close

Nb.BsrDI

Close

Nb.BssSI

Close

Nb.BtsI

Close

NciI

Close

NcoI

Close

NcoI-HF®

Close

NdeI

Close

NgoMIV

Close

NheI-HF®

Close

NlaIII

Close

NotI

Close

NotI-HF®

Close

NruI-HF®

Close

NsiI

Close

NsiI-HF®

Close

NspI

Close

Nt.AlwI

Close

Nt.BbvCI

Close

Nt.BsmAI

Close

Nt.BspQI

Close

Nt.BstNBI

Close

Nt.CviPII

Close

PacI

Close

PaeR7I

Close

PflFI

Close

PflMI

Close

PI-PspI

Close

PI-SceI

Close

PmeI

Close

PmlI

Close

PpuMI

Close

PshAI

Close

PstI

Close

PstI-HF®

Close

PvuI-HF®

Close

PvuII

Close

PvuII-HF®

Close

RsaI

Close

SacI-HF®

Close

SacII

Close

SalI

Close

SalI-HF®

Close

SapI

Close

SbfI-HF®

Close

ScaI-HF®

Close

SfiI

Close

SfoI

Close

SmaI

Close

SpeI-HF®

Close

SphI-HF®

Close

SspI-HF®

Close

StuI

Close

StyD4I

Close

StyI-HF®

Close

SwaI

Close

TaqI-v2

Close

TfiI

Close

TseI

Close

TspMI

Close

TspRI

Close

Tth111I

Close

WarmStart® Nt.BstNBI

Close

XbaI

Close

XhoI

Close

XmaI

Close

XmnI

Restriction Endonuclease Buffers & Diluents Products

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Diluent A (with rAlbumin)

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Diluent B (with rAlbumin)

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Diluent C (with rAlbumin)

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NEBuffer™ 1

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NEBuffer™ 2

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NEBuffer™ 3

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NEBuffer™ 4

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NEBuffer™ r2.1

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NEBuffer™ r3.1

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NEBuffer™ Set (EcoRI/SspI, DpnII)

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NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)

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rCutSmart™ Buffer

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Recombinant Albumin, Molecular Biology Grade

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S-adenosylmethionine (SAM)

Restriction Endonucleases: A Products
Close

AatII

Close

Acc65I

Close

AccI

Close

AciI

Close

AclI

Close

AcuI

Close

AfeI

Close

AflII

Close

AflIII

Close

AgeI-HF®

Close

AhdI

Close

AleI-v2

Close

AluI

Close

AlwI

Close

AlwNI

Close

ApaI

Close

ApaLI

Close

ApeKI

Close

ApoI-HF®

Close

AscI

Close

AseI

Close

AsiSI

Close

AvaI

Close

AvaII

Close

AvrII

Restriction Endonucleases: B Products
Close

BaeGI

Close

BaeI

Close

BamHI

Close

BamHI-HF®

Close

BanI

Close

BanII

Close

BbsI

Close

BbsI-HF®

Close

BbvCI

Close

BbvI

Close

BccI

Close

BceAI

Close

BcgI

Close

BciVI

Close

BclI

Close

BclI-HF

Close

BcoDI

Close

BfaI

Close

BfuAI

Close

BglI

Close

BglII

Close

BlpI

Close

BmgBI

Close

BmrI

Close

BmtI-HF®

Close

BpmI

Close

Bpu10I

Close

BpuEI

Close

BsaAI

Close

BsaBI

Close

BsaHI

Close

BsaI-HF®v2

Close

BsaJI

Close

BsaWI

Close

BsaXI

Close

BseRI

Close

BseYI

Close

BsgI

Close

BsiEI

Close

BsiHKAI

Close

BsiWI

Close

BsiWI-HF®

Close

BslI

Close

BsmAI

Close

BsmBI-v2

Close

BsmFI

Close

BsmI

Close

BsoBI

Close

Bsp1286I

Close

BspCNI

Close

BspDI

Close

BspEI

Close

BspHI

Close

BspMI

Close

BspQI

Close

BsrBI

Close

BsrDI

Close

BsrFI-v2

Close

BsrGI-HF®

Close

BsrI

Close

BssHII

Close

BssSI-v2

Close

BstAPI

Close

BstBI

Close

BstEII-HF®

Close

BstNI

Close

BstUI

Close

BstXI

Close

BstYI

Close

BstZ17I-HF®

Close

Bsu36I

Close

BtgI

Close

BtgZI

Close

BtsCI

Close

BtsIMutI

Close

BtsI-v2

Restriction Endonucleases: C-G Products
Close

Cac8I

Close

ClaI

Close

CspCI

Close

CviKI-1

Close

CviQI

Close

DdeI

Close

DpnI

Close

DpnII

Close

DraI

Close

DraIII-HF®

Close

DrdI

Close

EaeI

Close

EagI-HF®

Close

EarI

Close

EciI

Close

Eco53kI

Close

EcoNI

Close

EcoO109I

Close

EcoP15I

Close

EcoRI

Close

EcoRI-HF®

Close

EcoRV

Close

EcoRV-HF®

Close

Esp3I

Close

FatI

Close

FauI

Close

Fnu4HI

Close

FokI

Close

FseI

Close

FspI

Restriction Endonucleases: H-M Products
Close

HaeII

Close

HaeIII

Close

HgaI

Close

HhaI

Close

HincII

Close

HindIII

Close

HindIII-HF®

Close

HinfI

Close

HinP1I

Close

HpaI

Close

HpaII

Close

HphI

Close

Hpy166II

Close

Hpy188I

Close

Hpy188III

Close

Hpy99I

Close

HpyAV

Close

HpyCH4III

Close

HpyCH4IV

Close

HpyCH4V

Close

I-CeuI

Close

I-SceI

Close

KasI

Close

KpnI-HF®

Close

MboI

Close

MboII

Close

MfeI-HF®

Close

MluCI

Close

MluI-HF®

Close

MlyI

Close

MmeI

Close

MnlI

Close

MscI

Close

MseI

Close

MslI

Close

MspA1I

Close

MspI

Close

MspJI

Close

MwoI

Restriction Endonucleases: N-O Products
Close

NaeI

Close

NarI

Close

Nb.BbvCI

Close

Nb.BsmI

Close

Nb.BsrDI

Close

Nb.BssSI

Close

Nb.BtsI

Close

NciI

Close

NcoI

Close

NcoI-HF®

Close

NdeI

Close

NgoMIV

Close

NheI-HF®

Close

NlaIII

Close

NlaIV

Close

NmeAIII

Close

NotI

Close

NotI-HF®

Close

NruI-HF®

Close

NsiI

Close

NsiI-HF®

Close

NspI

Close

Nt.AlwI

Close

Nt.BbvCI

Close

Nt.BsmAI

Close

Nt.BspQI

Close

Nt.BstNBI

Close

Nt.CviPII

Close

WarmStart® Nt.BstNBI

Restriction Endonucleases: P-R Products
Close

PacI

Close

PaeR7I

Close

PaqCI®

Close

PciI

Close

PflFI

Close

PflMI

Close

PI-PspI

Close

PI-SceI

Close

PleI

Close

PluTI

Close

PmeI

Close

PmlI

Close

PpuMI

Close

PshAI

Close

PsiI-v2

Close

PspGI

Close

PspOMI

Close

PspXI

Close

PstI

Close

PstI-HF®

Close

PvuI-HF®

Close

PvuII

Close

PvuII-HF®

Close

RsaI

Close

RsrII

Restriction Endonucleases: S Products
Close

SacI-HF®

Close

SacII

Close

SalI

Close

SalI-HF®

Close

SapI

Close

Sau3AI

Close

Sau96I

Close

SbfI-HF®

Close

ScaI-HF®

Close

ScrFI

Close

SexAI

Close

SfaNI

Close

SfcI

Close

SfiI

Close

SfoI

Close

SgrAI

Close

SmaI

Close

SmlI

Close

SnaBI

Close

SpeI-HF®

Close

SphI

Close

SphI-HF®

Close

SrfI

Close

SspI-HF®

Close

StuI

Close

StyD4I

Close

StyI-HF®

Close

SwaI

Restriction Endonucleases: T-Z Products
Close

TaqI-v2

Close

TfiI

Close

TseI

Close

Tsp45I

Close

TspMI

Close

TspRI

Close

Tth111I

Close

XbaI

Close

XcmI

Close

XhoI

Close

XmaI

Close

XmnI

Close

ZraI

HF®, Nicking, Master Mix, Time-Saver & Other Products

High-Fidelity (HF®) Restriction Endonucleases

Close

AgeI-HF®

Close

ApoI-HF®

Close

BamHI-HF®

Close

BbsI-HF®

Close

BclI-HF

Close

BmtI-HF®

Close

BsaI-HF®v2

Close

BsiWI-HF®

Close

BsrGI-HF®

Close

BstEII-HF®

Close

BstZ17I-HF®

Close

DraIII-HF®

Close

EagI-HF®

Close

EcoRI-HF®

Close

EcoRV-HF®

Close

HindIII-HF®

Close

KpnI-HF®

Close

MfeI-HF®

Close

MluI-HF®

Close

NcoI-HF®

Close

NheI-HF®

Close

NotI-HF®

Close

NruI-HF®

Close

NsiI-HF®

Close

PstI-HF®

Close

PvuI-HF®

Close

PvuII-HF®

Close

SacI-HF®

Close

SalI-HF®

Close

SbfI-HF®

Close

ScaI-HF®

Close

SpeI-HF®

Close

SphI-HF®

Close

SspI-HF®

Close

StyI-HF®

Homing Endonucleases

Close

I-CeuI

Close

I-SceI

Close

PI-PspI

Close

PI-SceI

Nicking Endonucleases

Close

Nb.BbvCI

Close

Nb.BsmI

Close

Nb.BsrDI

Close

Nb.BssSI

Close

Nb.BtsI

Close

Nt.AlwI

Close

Nt.BbvCI

Close

Nt.BsmAI

Close

Nt.BspQI

Close

Nt.BstNBI

Close

Nt.CviPII

Close

WarmStart® Nt.BstNBI

Restriction Enzymes for Epigenetic Analysis

Close

DpnI

Close

DpnII

Close

HpaII

Close

MspI

Close

MspJI

Time-Saver™ Qualified Restriction Enzymes

Close

AatII

Close

Acc65I

Close

AccI

Close

AciI

Close

AclI

Close

AcuI

Close

AflII

Close

AgeI-HF®

Close

AhdI

Close

AluI

Close

AlwNI

Close

ApaI

Close

ApaLI

Close

ApeKI

Close

ApoI-HF®

Close

AscI

Close

AseI

Close

AvaI

Close

AvaII

Close

AvrII

Close

BaeGI

Close

BaeI

Close

BamHI

Close

BamHI-HF®

Close

BbsI

Close

BbsI-HF®

Close

BbvI

Close

BciVI

Close

BclI

Close

BclI-HF

Close

BcoDI

Close

BfuAI

Close

BglI

Close

BglII

Close

BlpI

Close

BmgBI

Close

BmtI-HF®

Close

BpmI

Close

BpuEI

Close

BsaAI

Close

BsaHI

Close

BsaI-HF®v2

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BsaWI

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BsaXI

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BseRI

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BsgI

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BsiEI

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BsiWI

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BsiWI-HF®

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BslI

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BsmAI

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BsmBI-v2

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BsmI

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BsoBI

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Bsp1286I

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BspCNI

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BspEI

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BspHI

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BspQI

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BsrBI

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BsrDI

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BsrI

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BssHII

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BstBI

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BstEII-HF®

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BstNI

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BstUI

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BstXI

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BstYI

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BstZ17I-HF®

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Bsu36I

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BtgI

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BtsCI

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Cac8I

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ClaI

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CspCI

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CviQI

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DdeI

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DpnI

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DpnII

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DraI

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DraIII-HF®

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DrdI

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EagI-HF®

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EarI

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Eco53kI

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EcoNI

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EcoO109I

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EcoP15I

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EcoRI

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EcoRI-HF®

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EcoRV

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EcoRV-HF®

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Esp3I

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Fnu4HI

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FseI

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FspI

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HaeIII

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HhaI

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HincII

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HindIII-HF®

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HinfI

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HinP1I

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HpaII

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HphI

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Hpy166II

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HpyAV

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HpyCH4IV

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HpyCH4V

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KpnI-HF®

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MboI

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MfeI-HF®

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MluCI

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MlyI

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MmeI

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MnlI

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MseI

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MslI

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MspA1I

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MspI

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MwoI

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NciI

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NcoI

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NcoI-HF®

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NdeI

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NgoMIV

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NheI-HF®

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NlaIII

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NotI

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NotI-HF®

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NsiI

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NspI

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PacI

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PaeR7I

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PflFI

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PflMI

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PmeI

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PmlI

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PpuMI

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PshAI

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PstI

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PstI-HF®

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PvuI-HF®

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PvuII

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PvuII-HF®

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RsaI

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SacI-HF®

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SacII

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SalI

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SalI-HF®

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SapI

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SbfI-HF®

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ScaI-HF®

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SfiI

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SfoI

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SmaI

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SpeI-HF®

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SphI-HF®

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SspI-HF®

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StuI

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StyD4I

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StyI-HF®

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SwaI

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TaqI-v2

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TfiI

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TseI

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TspMI

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TspRI

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Tth111I

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XbaI

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XhoI

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XmaI

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XmnI

Restriction Endonuclease Buffers & Diluents Products
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Diluent A (with rAlbumin)

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Diluent B (with rAlbumin)

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Diluent C (with rAlbumin)

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NEBuffer™ 1

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NEBuffer™ 2

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NEBuffer™ 3

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NEBuffer™ 4

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NEBuffer™ r2.1

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NEBuffer™ r3.1

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NEBuffer™ Set (EcoRI/SspI, DpnII)

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NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)

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rCutSmart™ Buffer

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Recombinant Albumin, Molecular Biology Grade

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S-adenosylmethionine (SAM)

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