
Restriction Endonucleases
The Leader in the Discovery and Production of Restriction Enzymes
With > 45 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.
NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. With the industry’s largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.
NEB feels that moving away from animal-containing products is a step in the right direction. We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free! Learn more at www.neb.com/BSA-free.
For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.
All of NEB's Restriction enzymes have transitioned to a new buffer system. Visit NEBCutSmart.com for further details.
Convenience
- >210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer.
- >180 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight.
- Choose from >265 restriction enzymes, the largest selection commercially available.
Performance
- Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in rCutSmart Buffer. A vial of 6X Purple Loading Dye is included with most restriction enzymes.
- All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.
Physical Purity Enzymes are evaluated by SDS-PAGE and silver-stained gels to ensure the highest levels of purity and the absence of contaminating proteins.
DNA Contamination Enzymes are screened by qPCR to ensure no contaminating genomic DNA is present. The specification for this assay is less than one E.coli genome per sample. Exonuclease Activity Using radioactively labelled DNA substrate and/or state-of-the-art capillary electrophoresis-based assays with fluorescently-labelled substrates, NEB is able to detect very low levels of exonuclease activity. Endonuclease Activity To ensure that there are no contaminating enzymes that could cause nickng or non-specific nuclease degradation, reagents are incubated with supercoiled plasmid DNA for 4 hours to demonstrate the absence of endonuclease contamination. Non-Specific DNase Activity Enzymes are incubated overnight with Lambda DNA to confirm that there is no additional non-specific nuclease activity present. Cloning QC (Ligation and Re-cutting) A DNA template is over-digested by the appropriate restriction enzyme and the percentage of DNA fragments ligated and re-cut are determined by agarose gel electrophoresis. Cloning QC (Blue-white Screening) A DNA plasmid is over-digested by the appropriate restriction enzyme and the linearized plasmid DNA is ligated and transformed into an E.coli strain with greater than 99% correct transformants, as determined by a blue-white screen.
Use Enzyme Finder to select restriction enzyme by name, sequence, overhang or type.
Cut Smarter with Restriction Enzymes from NEB
With over 45 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.
Convenience
- A vial of 6X Purple Load Dye is included with most restriction enzymes.
- Over 210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer.
- >180 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight.
- Choose from >265 restriction enzymes, the largest selection commercially available.
Performance
- Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in rCutSmart Buffer. A vial of 6X Purple Load Dye is included with every HF restriction enzyme.
- All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.
Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.
Choose Product:
Restriction Endonucleases: A
Restriction Endonucleases B
Restriction Endonucleases C G
Restriction Endonucleases H M
Restriction Endonucleases: N-O
Restriction Endonucleases P R
Restriction Endonucleases S
Restriction Endonucleases T Z
HF®, Nicking, Master Mix, Time-Saver & Other
Restriction Endonuclease Buffers & Diluents
High-Fidelity (HF®) Restriction Endonucleases
Homing Endonucleases
Nicking Endonucleases
Restriction Enzymes for Epigenetic Analysis
Time-Saver™ Qualified Restriction Enzymes
Choose Type:
- Restriction Endonucleases: A
- Restriction Endonucleases: B
- Restriction Endonucleases: C-G
- Restriction Endonucleases: H-M
- Restriction Endonucleases: N-O
- Restriction Endonucleases: P-R
- Restriction Endonucleases: S
- Restriction Endonucleases: T-Z
- HF®, Nicking, Master Mix, Time-Saver & Other
- Restriction Endonuclease Buffers & Diluents
- Digestion of Agarose-Embedded DNA
- Standard Digest Using RE-Mix®
- Double Digest Protocol using Two RE-Mix® Enzymes
- Optimizing Restriction Endonuclease Reactions
- Double Digest Protocol using One RE-Mix and One Standard Restriction Enzyme
- Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
- Double Digest Protocol with Standard Restriction Enzymes
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Restriction Endonucleases: Molecular Cloning and Beyond
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Type II Restriction Enzymes: What You Need to Know | NEB
Read about Type II restriction enzymes and the distinguishing properties of the four principle subtypes.
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A Modern Day Gene Genie Sir Richard Roberts on Rebase
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Restriction Enzyme Cleavage: ‘single-site’ enzymes and ‘multi-site’ enzymes
Restriction enzymes are proteins used to fragment and clone DNA, but their biological function is to protect bacteria and archaea against viral infections.
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Whole genome assembly from next generation sequencing data using restriction and nicking enzymes in optical mapping and proximity-based ligation strategies
High throughput sequencing methods have revolutionized genomic analysis by producing millions of sequence reads from an organism’s DNA at an ever decreasing cost.
- NEB Restriction Enzyme Activity Poster
- DNA Sequences and Maps Tool
- REBASE®
- Alphabetized List of Recognition Sequences
- Cleavage Of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Enzymes with Multiple Recognition Sequences
- Enzymes with Nonpalindromic Sequences
- Frequencies of Restriction Sites
- Interrupted Palindromes
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- Recleavable Blunt Ends
- Recleavable Filled-in 5' Overhangs
- Time-Saver™ Qualified Enzymes
- Type IIS Restriction Enzymes
- Why Choose Recombinant Enzymes?
- Restriction Enzyme Troubleshooting Guide
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Alteration of Apparent Recognition Specificities Using Methylases
- Cleavage Close to the End of DNA Fragments
- Dam and Dcm Methylases of E. coli
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Effects of CpG Methylation on Restriction Enzyme Cleavage
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Restriction Enzymes for Droplet Digital PCR (ddPCR)
- Restriction Enzymes requiring multi-sites for efficient cleavage
- Restriction of Foreign DNA by E. coli K-12
- Site Preferences
- Star Activity
- Traditional Cloning Quick Guide
- Crystal Structure of the 8 bp-Specific Restriction Enzyme SwaI (2015)
Feature Articles
Brochures
Web Tools
Selection Tools
Troubleshooting Guides
Usage Guidelines
Posters
- Morgan, R.D., Luyten, Y.A., Johnson, S.A., Clough, E.M., Clark, T.A. and Roberts, R.J. (2016) Novel m4C modification in type I restriction-modification systems. Nucleic Acids Res; Nov, 2;44(19):9413-9425. PubMedID: 27580720
- Fu YB, Peterson G. W., Dong Y (2016) Increasing Genome Sampling and Improving SNP Genotyping for Genotyping-by-Sequencing with New Combinations of Restriction Enzymes G3 (Bethesda); 6:4, 845-846. PubMedID: 26818077
- Blow, M.J., Clark, T.A., Daum, C.G., Deutschbauer, A.M., Fomenkov, A., Fries, R., Froula, J., Kang, D.D., Malmstrom, R.R., Morgan, R.D., Posfai, J., Singh, K., Visel, A., Wetmore, K., Zhao, Z., Rubin, E.M., Korlach, J., Pennacchio, L.A. and Roberts, R.J. (2016) The Epigenomic Landscape of Prokaryotes. PLoS Genet; Feb 12;12(2):e1005854, PubMedID: 26870957, DOI: 10.1371/journal.pgen.1005854
- Callahan, S.J., Luyten, Y.A., Gupta, Y.K., Wilson, G.G., Roberts, R.J., Morgan, R.D. and Aggarwal, A.K. (2016) Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. PLoS Biol; Apr 15;14(4):e1002442, PubMedID: 27082731
- Kamps-Hughes, N., Quimby, A., Zhu, Z., Johnson, E.A. (2013) Massively parallel characterization of restriction endonucleases Nucleic Acids Res ; 41(11), e119. PubMedID: 23605040, DOI: 10.1093/nar/gkt257
- Shah, S., Sanchez, J., Stewart, A., et al. (2015) Probing the Run-On Oligomer of Activated SgrAI Bound to DNA PLoS One; 10(4), PubMedID: 25880668, DOI: 10.1371/journal.pone.0124783.
- Fomenkov, A., Lunnen, K.D., Zhu, Z., Anton, B.P., Wilson, G.G., Vincze, T. and Roberts, R.J. (2015) Complete genome sequence and methylome analysis of bacillus strain x1 Genome Announc; 3(1), PubMedID: 25700417
- Roberts, R.J., Vincze, T., Posfai, J., Macelis, D. (2014) REBASE - A database for DNA restriction and modification: enzymes, genes and genomes Nucleic Acids Res; PubMedID: 25378308
- Loenen, W.A., Raleigh, E.A. (2014) The other face of restriction: modification-dependent enzymes. Nucleic Acids Res; 42, 56-69. PubMedID: 23990325, DOI: doi: 10.1093/nar/gkt847
- Roberts, R.J., Vincze, T., Posfai, J., Macelis, D. (2015) REBASE - A database for DNA restriction and modification: enzymes, genes and genomes Nucleic Acids Res; 43, D298-D299. PubMedID: 25378308
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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