Icon Descriptions
The gene encoding this enzyme has been cloned at New England Biolabs, Inc.
This enzyme is purified from a recombinant source.
This enzyme has been engineered.
This enzyme will digest unit substrate in 5-15 minutes under recommended reaction conditions, and can also be used safely in overnight digestions.
Indicates that the polymerase is high fidelity.
Indicates that the polymerase is a hot start/warm start enzyme.
This polymerase is used for PCR.
Ta = Tm + 3°C for the polymerase.
Ta = Tm – 5°C for the polymerase.
Indicates which reaction buffer is supplied with the enzyme for optimal activity. Enzymes with buffer requirements not met by one of the four standard NEBuffers are supplied with their own unique NEBuffer (NEB U). NEBuffers are color-coded (NEBuffer r1.1 & 1.1 - yellow, NEBuffer r2. & 2.1 - blue, NEBuffer r3.1 & 3.1 - red, rCutSmart & CutSmart - green) and supplied as 10X stocks with each enzyme.
Indicates which diluent buffer (A, B or C) is recommended for making dilutions of restriction enzymes.
Indicates the product can amplify DNA directly with no need for extraction.
Indicates the enzyme's optimum incubation temperature.
Indicates that the restriction enzyme requires two or more sites for optimal cleavage.
This enzyme is supplied with a separate tube of S-adenosylmethionine (SAM). To obtain 100% activity SAM should be added to the 1X reaction mix as indicated. When required, a concentrated stock of SAM is supplied with the enzyme.
This enzyme is supplied with a separate tube of bovine serum albumin (BSA). To obtain 100% activity BSA should be added to the 1X reaction mix to a final concentration as indicated.
This enzyme is supplied with a separate tube of Recombinant Albumin (rAlbumin). To obtain 100% activity rAlbumin should be added to the 1X reaction mix to a final concentration as indicated.
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by either the dam or dcm or CpG methylase.
Indicates whether or not the enzyme can be heat inactivated. Enzymes are first tested by incubation at 65°C for 20 minutes; any enzyme not inactivated at 65°C is then tested by incubation at 80°C for 20 minutes.
This enzyme has passed the blue/white selection assay. This assay is performed on enzymes with single sites in cloning vectors, it is an extremely sensitive measure of the integrity of DNA fragment ends after excess digestion with the enzyme.
