Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
r1.1 | r2.1 | r3.1 | rCutSmart | ||||||||||||
AatII | |
GACGT/C | rCutSmart™ Buffer | <10 | 50* | 50 | 100 | 80°C | 37°C | B | λ DNA | ||||
AccI | |
GT/MKAC | rCutSmart™ Buffer | 50 | 50 | 10 | 100 | 80°C | 37°C | A | λ DNA | ||||
Acc65I | |
G/GTACC | NEBuffer™ r3.1 | 10 | 75* | 100 | 25 | 65°C | 37°C | A | pBC4 DNA | ||||
AciI | |
CCGC(-3/-1) | rCutSmart™ Buffer | <10 | 25 | 100 | 100 | 65°C | 37°C | A | λ DNA | ||||
AclI | |
AA/CGTT | rCutSmart™ Buffer | <10 | <10 | <10 | 100 | No | 37°C | B | λ DNA | ||||
AcuI | |
CTGAAG(16/14) | rCutSmart™ Buffer | 50 | 100 | 50 | 100 | 65°C | 37°C | B | λ DNA | 1, b, d | |||
AfeI | |
AGC/GCT | rCutSmart™ Buffer | 25 | 100 | 25 | 100 | 65°C | 37°C | B | pXba DNA | ||||
AflII | |
C/TTAAG | rCutSmart™ Buffer | 50 | 100 | 10 | 100 | 65°C | 37°C | A | ΦX174 RF I DNA | ||||
AflIII | |
A/CRYGT | NEBuffer™ r3.1 | 10 | 50 | 100 | 50 | 80°C | 37°C | B | λ DNA | ||||
AgeI-HF® | |
A/CCGGT | rCutSmart™ Buffer | 100 | 50 | 10 | 100 | 65°C | 37°C | A | λ DNA | ||||
AhdI | |
GACNNN/NNGTC | rCutSmart™ Buffer | 25 | 25 | 10 | 100 | 65°C | 37°C | A | λ DNA | a | |||
AleI-v2 | |
CACNN/NNGTG | rCutSmart™ Buffer | <10 | <10 | <10 | 100 | 65°C | 37°C | B | λ DNA | ||||
AluI | |
AG/CT | rCutSmart™ Buffer | 25 | 100 | 50 | 100 | 80°C | 37°C | B | λ DNA | b | |||
AlwI | |
GGATC(4/5) | rCutSmart™ Buffer | 50 | 50 | 10 | 100 | No | 37°C | A | λ DNA (dam-) | 1, b, d | |||
AlwNI | |
CAGNNN/CTG | rCutSmart™ Buffer | 10 | 100 | 50 | 100 | 80°C | 37°C | A | λ DNA | ||||
ApaI | |
GGGCC/C | rCutSmart™ Buffer | 25 | 25 | <10 | 100 | 65°C | 37°C | A | pXba DNA | ||||
ApaLI | |
G/TGCAC | rCutSmart™ Buffer | 100 | 100 | 10 | 100 | No | 37°C | A | λ DNA (HindIII digest) | ||||
ApeKI | |
G/CWGC | NEBuffer™ r3.1 | 25 | 50 | 100 | 10 | No | 75°C | B | λ DNA | ||||
ApoI-HF | |
R/AATTY | rCutSmart™ Buffer | 10 | 100 | 10 | 100 | 80°C | 37°C | B | λ DNA | ||||
AscI | |
GG/CGCGCC | rCutSmart™ Buffer | <10 | 10 | 10 | 100 | 80°C | 37°C | A | λ DNA | ||||
AseI | |
AT/TAAT | NEBuffer™ r3.1 | <10 | 50* | 100 | 10 | 65°C | 37°C | B | λ DNA | 3 | |||
AsiSI | |
GCGAT/CGC | rCutSmart™ Buffer | 100 | 100 | 25 | 100 | 80°C | 37°C | B | XhoI digested pXba | 2, b | |||
AvaI | |
C/YCGRG | rCutSmart™ Buffer | <10 | 100 | 25 | 100 | 80°C | 37°C | A | λ DNA | ||||
AvaII | |
G/GWCC | rCutSmart™ Buffer | 50 | 75 | 10 | 100 | 80°C | 37°C | A | λ DNA | ||||
AvrII | |
C/CTAGG | rCutSmart™ Buffer | 100 | 50 | 50 | 100 | No | 37°C | B | λ DNA (HindIII digest) | ||||
BaeGI | |
GKGCM/C | NEBuffer™ r3.1 | 75 | 75 | 100 | 25 | 80°C | 37°C | A | λ DNA | ||||
BaeI | |
(10/15)ACNNNNGTAYC(12/7) | rCutSmart™ Buffer | 50 | 100 | 50 | 100 | 65°C | 37°C | A | λ DNA | e | |||
BamHI § | |
G/GATCC | NEBuffer™ r3.1 | 75* | 100* | 100 | 100* | No | 37°C | A | λ DNA | 3 | |||
BamHI-HF® | |
G/GATCC | rCutSmart™ Buffer | 100 | 50 | 10 | 100 | No | 37°C | A | λ DNA | ||||
BanI | |
G/GYRCC | rCutSmart™ Buffer | 10 | 25 | <10 | 100 | 65°C | 37°C | A | λ DNA | 1 | |||
BanII | |
GRGCY/C | rCutSmart™ Buffer | 100 | 100 | 50 | 100 | 80°C | 37°C | A | λ DNA | 2 | |||
BbsI § | |
GAAGAC(2/6) | NEBuffer™ r2.1 | 100 | 100 | 25 | 75 | 65°C | 37°C | B | λ DNA | ||||
BbsI-HF® | |
GAAGAC(2/6) | rCutSmart™ Buffer | 10 | 10 | 10 | 100 | 65°C | 37°C | B | λ DNA | ||||
BbvCI | |
CCTCAGC(-5/-2) | rCutSmart™ Buffer | 10 | 100 | 50 | 100 | No | 37°C | B | λ DNA | 1, a | |||
BbvI | |
GCAGC(8/12) | rCutSmart™ Buffer | 100 | 100 | 25 | 100 | 65°C | 37°C | B | pBR322 DNA | 3 | |||
BccI | |
CCATC(4/5) | rCutSmart™ Buffer | 100 | 50 | 10 | 100 | 65°C | 37°C | A | pXba DNA | 3, b | |||
BceAI | |
ACGGC(12/14) | NEBuffer™ r3.1 | 100* | 100* | 100 | 100* | 65°C | 37°C | A | pBR322 DNA | 1 | |||
BcgI | |
(10/12)CGANNNNNNTGC(12/10) | NEBuffer™ r3.1 | 10 | 75* | 100 | 50* | 65°C | 37°C | A | λ DNA | e | |||
BciVI | |
GTATCC(6/5) | rCutSmart™ Buffer | 100 | 25 | <10 | 100 | 80°C | 37°C | C | λ DNA | b | |||
BclI § | |
T/GATCA | NEBuffer™ r3.1 | 50 | 100 | 100 | 75 | No | 37°C | A | λ DNA (dam-) | ||||
BclI-HF | |
T/GATCA | rCutSmart™ Buffer | 100 | 100 | 10 | 100 | 65°C | 37°C | B | λ DNA (dam-) | ||||
BcoDI | |
GTCTC(1/5) | rCutSmart™ Buffer | 50 | 75 | 75 | 100 | No | 37°C | B | λ DNA | ||||
BfaI | |
C/TAG | rCutSmart™ Buffer | <10 | 10 | <10 | 100 | 80°C | 37°C | B | λ DNA | 2, b | |||
BfuAI | |
ACCTGC(4/8) | NEBuffer™ r3.1 | <10 | 25 | 100 | 10 | 65°C | 50°C | B | λ DNA | 3 | |||
BglI | |
GCCNNNN/NGGC | NEBuffer™ r3.1 | 10 | 25 | 100 | 10 | 65°C | 37°C | B | λ DNA | ||||
BglII | |
A/GATCT | NEBuffer™ r3.1 | 10 | 10 | 100 | <10 | No | 37°C | A | λ DNA | ||||
BlpI | |
GC/TNAGC | rCutSmart™ Buffer | 50 | 100 | 10 | 100 | No | 37°C | A | λ DNA | d | |||
BmgBI | |
CACGTC(-3/-3) | NEBuffer™ r3.1 | <10 | 10 | 100 | 10 | 65°C | 37°C | B | λ DNA | 3, b, d | |||
BmrI | |
ACTGGG(5/4) | NEBuffer™ r2.1 | 75 | 100 | 75 | 100* | 65°C | 37°C | B | λ DNA (HindIII digest) | b | |||
BmtI-HF® | |
GCTAG/C | rCutSmart™ Buffer | 50 | 100 | 10 | 100 | 65°C | 37°C | B | pXba DNA | ||||
BpmI | |
CTGGAG(16/14) | NEBuffer™ r3.1 | 75 | 100 | 100 | 100* | 65°C | 37°C | B | λ DNA | 2 | |||
BpuEI | |
CTTGAG(16/14) | rCutSmart™ Buffer | 50* | 100 | 50* | 100 | 65°C | 37°C | B | λ DNA | d | |||
Bpu10I | |
CCTNAGC(-5/-2) | NEBuffer™ r3.1 | 10 | 25 | 100 | 25 | 80°C | 37°C | B | λ DNA | 3, b, d | |||
BsaAI | |
YAC/GTR | rCutSmart™ Buffer | 100 | 100 | 100 | 100 | No | 37°C | C | λ DNA | ||||
BsaBI | |
GATNN/NNATC | rCutSmart™ Buffer | 50 | 100 | 75 | 100 | 80°C | 60°C | B | λ DNA (dam-) | 2 | |||
BsaHI | |
GR/CGYC | rCutSmart™ Buffer | 50 | 100 | 100 | 100 | 80°C | 37°C | C | λ DNA | ||||
BsaI-HF®v2 | |
GGTCTC(1/5) | rCutSmart™ Buffer | 100 | 100 | 100 | 100 | 80°C | 37°C | B | pXba DNA | ||||
BsaJI | |
C/CNNGG | rCutSmart™ Buffer | 50 | 100 | 100 | 100 | 80°C | 60°C | A | λ DNA | ||||
BsaWI | |
W/CCGGW | rCutSmart™ Buffer | 10 | 100 | 50 | 100 | 80°C | 60°C | A | λ DNA | ||||
BsaXI | |
(9/12)ACNNNNNCTCC(10/7) | rCutSmart™ Buffer | 50* | 100* | 10 | 100 | No | 37°C | C | λ DNA | e | |||
BseRI | |
GAGGAG(10/8) | rCutSmart™ Buffer | 100 | 100 | 75 | 100 | 80°C | 37°C | A | λ DNA | d | |||
BseYI | |
CCCAGC(-5/-1) | NEBuffer™ r3.1 | 10 | 50 | 100 | 50 | 80°C | 37°C | B | λ DNA | d | |||
BsgI | |
GTGCAG(16/14) | rCutSmart™ Buffer | 25 | 50 | 25 | 100 | 65°C | 37°C | B | λ DNA | d | |||
BsiEI | |
CGRY/CG | rCutSmart™ Buffer | 25 | 50 | <10 | 100 | No | 60°C | A | λ DNA | ||||
BsiHKAI | |
GWGCW/C | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | No | 65°C | A | λ DNA | ||||
BsiWI § | |
C/GTACG | NEBuffer™ r3.1 | 25 | 50* | 100 | 25 | 80°C | 55°C | B | ΦX174 DNA | ||||
BsiWI-HF® | |
C/GTACG | rCutSmart™ Buffer | 50 | 100 | 10 | 100 | 80°C | 37°C | B | ΦX174 DNA | ||||
BslI | |
CCNNNNN/NNGG | rCutSmart™ Buffer | 50 | 75 | 100 | 100 | No | 37°C | A | λ DNA | b | |||
BsmAI | |
GTCTC(1/5) | rCutSmart™ Buffer | 50 | 100 | 100 | 100 | No | 37°C | B | λ DNA | ||||
BsmBI-v2 | |
CGTCTC | NEBuffer™ r3.1 | <10 | 50 | 100 | 25 | 80°C | 55°C | B | λ DNA | ||||
BsmFI | |
GGGAC(10/14) | rCutSmart™ Buffer | 25 | 50 | 50 | 100 | 80°C | 37°C | A | pBR322 DNA | 1 | |||
BsmI | |
GAATGC(1/-1) | rCutSmart™ Buffer | 25 | 100 | <10 | 100 | 80°C | 65°C | A | λ DNA | ||||
BsoBI | |
C/YCGRG | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | 80°C | 37°C | A | λ DNA | ||||
BspCNI | |
CTCAG(9/7) | rCutSmart™ Buffer | 100 | 75 | 10 | 100 | 80°C | 37°C | A | λ DNA | b | |||
BspDI | |
AT/CGAT | rCutSmart™ Buffer | 25 | 75 | 50 | 100 | 80°C | 37°C | A | λ DNA | ||||
BspEI | |
T/CCGGA | NEBuffer™ r3.1 | <10 | 10 | 100 | <10 | 80°C | 37°C | B | λ DNA (dam-) | ||||
BspHI | |
T/CATGA | rCutSmart™ Buffer | 10 | 50 | 25 | 100 | 80°C | 37°C | A | λ DNA | ||||
Bsp1286I | |
GDGCH/C | rCutSmart™ Buffer | 25 | 25 | 25 | 100 | 65°C | 37°C | A | λ DNA | 3 | |||
BspMI | |
ACCTGC(4/8) | NEBuffer™ r3.1 | 10 | 50* | 100 | 10 | 65°C | 37°C | B | λ DNA | ||||
BspQI | |
GCTCTTC(1/4) | NEBuffer™ r3.1 | 100* | 100* | 100 | 100* | 80°C | 50°C | B | λ DNA | 3 | |||
R3712 BspQI-HF | |
rCutSmart™ Buffer | 25 | 100 | 50 | 100 | 80°C | 37°C | B | ||||||
BsrBI | |
CCGCTC(-3/-3) | rCutSmart™ Buffer | 50 | 100 | 100 | 100 | 80°C | 37°C | A | λ DNA | d | |||
BsrDI | |
GCAATG(2/0) | NEBuffer™ r2.1 | 10 | 100 | 75 | 25 | 80°C | 37°C | A | λ DNA | 3, d | |||
BsrFI-v2 | |
R/CCGGY | rCutSmart™ Buffer | 25 | 25 | 0 | 100 | No | 37°C | C | pBR322 DNA | ||||
BsrGI-HF® | |
T/GTACA | rCutSmart™ Buffer | 10 | 100 | 100 | 100 | 80°C | 37°C | A | λ DNA | ||||
BsrI | |
ACTGG(1/-1) | NEBuffer™ r3.1 | <10 | 50 | 100 | 10 | 80°C | 65°C | B | ΦX174 DNA | b | |||
BssHII | |
G/CGCGC | rCutSmart™ Buffer | 100 | 100 | 100 | 100 | 65°C | 37°C | B | λ DNA | ||||
BssSI-v2 | |
CACGAG(-5/-1) | rCutSmart™ Buffer | 10 | 25 | <10 | 100 | No | 37°C | B | λ DNA | ||||
BstAPI | |
GCANNNN/NTGC | rCutSmart™ Buffer | 50 | 100 | 25 | 100 | 80°C | 60°C | A | λ DNA | b | |||
BstBI | |
TT/CGAA | rCutSmart™ Buffer | 75 | 100 | 10 | 100 | No | 65°C | A | λ DNA | ||||
BstEII-HF® | |
G/GTNACC | rCutSmart™ Buffer | <10 | 10 | <10 | 100 | No | 37°C | A | λ DNA | ||||
BstNI | |
CC/WGG | NEBuffer™ r3.1 | 10 | 100 | 100 | 75 | No | 60°C | A | λ DNA | a | |||
BstUI | |
CG/CG | rCutSmart™ Buffer | 50 | 100 | 25 | 100 | No | 60°C | A | λ DNA | b | |||
BstXI | |
CCANNNNN/NTGG | NEBuffer™ r3.1 | <10 | 50 | 100 | 25 | 80°C | 37°C | B | λ DNA | 3 | |||
BstYI | |
R/GATCY | rCutSmart™ Buffer | 25 | 100 | 75 | 100 | No | 60°C | A | λ DNA | ||||
BstZ17I-HF® | |
GTATAC | rCutSmart™ Buffer | 100 | 100 | 10 | 100 | No | 37°C | A | λ DNA | ||||
Bsu36I | |
CC/TNAGG | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | 80°C | 37°C | C | λ DNA (HindIII digest) | b | |||
BtgI | |
C/CRYGG | rCutSmart™ Buffer | 50 | 100 | 100 | 100 | 80°C | 37°C | B | pBR322 DNA | ||||
BtgZI | |
GCGATG(10/14) | rCutSmart™ Buffer | 10 | 25 | <10 | 100 | 80°C | 60°C | A | λ DNA | 3, b, d | |||
BtsCI | |
GGATG(2/0) | rCutSmart™ Buffer | 10 | 100 | 25 | 100 | 80°C | 50°C | B | λ DNA | ||||
BtsIMutI | |
CAGTG(2/0) | rCutSmart™ Buffer | 100 | 50 | 10 | 100 | 80°C | 55°C | A | pUC19 DNA | b | |||
BtsI-v2 | |
GCAGTG(2/0) | rCutSmart™ Buffer | 100 | 100 | 25 | 100 | No | 37°C | A | λ DNA | 1 | |||
Cac8I | |
GCN/NGC | rCutSmart™ Buffer | 50 | 75 | 100 | 100 | 65°C | 37°C | B | λ DNA | b | |||
ClaI | |
AT/CGAT | rCutSmart™ Buffer | 10 | 50 | 50 | 100 | 65°C | 37°C | A | λ DNA (dam-) | ||||
CspCI | |
(11/13)CAANNNNNGTGG(12/10) | rCutSmart™ Buffer | 10 | 100 | 10 | 100 | 65°C | 37°C | A | λ DNA | e | |||
CviKI-1 | |
RG/CY | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | No | 37°C | A | pBR322 DNA | 1, b | |||
CviQI | |
G/TAC | NEBuffer™ r3.1 | 75 | 100* | 100 | 75* | No | 25°C | C | λ DNA | b | |||
DdeI | |
C/TNAG | rCutSmart™ Buffer | 75 | 100 | 100 | 100 | 65°C | 37°C | B | λ DNA | ||||
DpnI | |
GA/TC | rCutSmart™ Buffer | 100 | 100 | 75 | 100 | 80°C | 37°C | B | pBR322 DNA (dam methylated) | b | |||
DpnII | |
/GATC | NEBuffer™ DpnII | 25 | 25 | 100* | 25 | 65°C | 37°C | B | λ DNA (dam-) | ||||
DraI | |
TTT/AAA | rCutSmart™ Buffer | 75 | 75 | 50 | 100 | 65°C | 37°C | A | λ DNA | ||||
DraIII-HF® | |
CACNNN/GTG | rCutSmart™ Buffer | <10 | 50 | 10 | 100 | No | 37°C | B | λ DNA | b | |||
DrdI | |
GACNNNN/NNGTC | rCutSmart™ Buffer | 25 | 50 | 10 | 100 | 65°C | 37°C | A | pUC19 DNA | 3 | |||
EaeI | |
Y/GGCCR | rCutSmart™ Buffer | 10 | 50 | <10 | 100 | 65°C | 37°C | A | λ DNA | b | |||
EagI-HF® | |
C/GGCCG | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | 65°C | 37°C | B | pXba DNA | ||||
EarI | |
CTCTTC(1/4) | rCutSmart™ Buffer | 50 | 10 | <10 | 100 | 65°C | 37°C | B | λ DNA | b, d | |||
EciI | |
GGCGGA(11/9) | rCutSmart™ Buffer | 100 | 50 | 50 | 100 | 65°C | 37°C | A | λ DNA | 2 | |||
Eco53kI | |
GAG/CTC | rCutSmart™ Buffer | 100 | 100 | <10 | 100 | 65°C | 37°C | A | pXba DNA | 3, b | |||
EcoNI | |
CCTNN/NNNAGG | rCutSmart™ Buffer | 50 | 100 | 75 | 100 | 65°C | 37°C | A | λ DNA | b | |||
EcoO109I | |
RG/GNCCY | rCutSmart™ Buffer | 50 | 100 | 50 | 100 | 65°C | 37°C | A | λ DNA (HindIII digest) | 3 | |||
EcoP15I | |
CAGCAG(25/27) | NEBuffer™ r3.1 + ATP | 75 | 100 | 100 | 100 | 65°C | 37°C | A | pUC19 DNA | e | |||
EcoRI § | |
G/AATTC | NEBuffer™ EcoRI/SspI | 25 | 100* | 50 | 50* | 65°C | 37°C | C | λ DNA | ||||
EcoRI-HF® | |
G/AATTC | rCutSmart™ Buffer | 10 | 100 | <10 | 100 | 65°C | 37°C | C | λ DNA | ||||
EcoRV § | |
GAT/ATC | NEBuffer™ r3.1 | 10 | 50 | 100 | 10 | 80°C | 37°C | A | λ DNA | ||||
EcoRV-HF® | |
GAT/ATC | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | 65°C | 37°C | B | λ DNA | ||||
Esp3I | |
CGTCTC(1/5) | rCutSmart™ Buffer | 100 | 100 | <10 | 100 | 65°C | 37°C | B | λ DNA | ||||
FatI | |
/CATG | NEBuffer™ r2.1 | 10 | 100 | 50 | 50 | 80°C | 55°C | A | pUC19 DNA | ||||
FauI | |
CCCGC(4/6) | rCutSmart™ Buffer | 100 | 50 | 10 | 100 | 65°C | 55°C | A | λ DNA | 3, b, d | |||
Fnu4HI | |
GC/NGC | rCutSmart™ Buffer | <10 | <10 | <10 | 100 | No | 37°C | A | λ DNA | a | |||
FokI | |
GGATG(9/13) | rCutSmart™ Buffer | 100 | 100 | 75 | 100 | 65°C | 37°C | A | λ DNA | 3, b, d | |||
FseI | |
GGCCGG/CC | rCutSmart™ Buffer | 100 | 75 | <10 | 100 | 65°C | 37°C | B | pBC4 DNA | ||||
FspI | |
TGC/GCA | rCutSmart™ Buffer | 10 | 100 | 10 | 100 | No | 37°C | C | λ DNA | b | |||
HaeII | |
RGCGC/Y | rCutSmart™ Buffer | 25 | 100 | 10 | 100 | 80°C | 37°C | A | λ DNA | ||||
HaeIII | |
GG/CC | rCutSmart™ Buffer | 50 | 100 | 25 | 100 | 80°C | 37°C | A | λ DNA | ||||
HgaI | |
GACGC(5/10) | NEBuffer™ r1.1 | 100 | 100 | 25 | 100* | 65°C | 37°C | A | ΦX174 DNA | 1 | |||
HhaI | |
GCG/C | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | 65°C | 37°C | A | λ DNA | ||||
HincII | |
GTY/RAC | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | 65°C | 37°C | B | λ DNA | ||||
HindIII § | |
A/AGCTT | NEBuffer™ r2.1 | 25 | 100 | 50 | 50 | 80°C | 37°C | B | λ DNA | ||||
HindIII-HF® | |
A/AGCTT | rCutSmart™ Buffer | 10 | 100 | 10 | 100 | 80°C | 37°C | B | λ DNA | ||||
HinfI | |
G/ANTC | rCutSmart™ Buffer | 50 | 100 | 100 | 100 | 80°C | 37°C | A | λ DNA | ||||
HinP1I | |
G/CGC | rCutSmart™ Buffer | 100 | 100 | 100 | 100 | 65°C | 37°C | A | λ DNA | ||||
HpaI | |
GTT/AAC | rCutSmart™ Buffer | <10 | 75* | 25 | 100 | No | 37°C | A | λ DNA | 1 | |||
HpaII | |
C/CGG | rCutSmart™ Buffer | 100 | 50 | <10 | 100 | 80°C | 37°C | A | λ DNA | ||||
HphI | |
GGTGA(8/7) | rCutSmart™ Buffer | 50 | 50 | <10 | 100 | 65°C | 37°C | B | λ DNA | 1, b, d | |||
HpyAV | |
CCTTC(6/5) | rCutSmart™ Buffer | 100 | 100 | 25 | 100 | 65°C | 37°C | λ DNA | 3, b, d | ||||
HpyCH4III | |
ACN/GT | rCutSmart™ Buffer | 100 | 25 | <10 | 100 | 65°C | 37°C | A | λ DNA | b | |||
HpyCH4IV | |
A/CGT | rCutSmart™ Buffer | 100 | 50 | 25 | 100 | 65°C | 37°C | A | pUC19 DNA | ||||
HpyCH4V | |
TG/CA | rCutSmart™ Buffer | 50 | 50 | 25 | 100 | 65°C | 37°C | A | λ DNA | ||||
Hpy188I | |
TCN/GA | rCutSmart™ Buffer | 25 | 100 | 50 | 100 | 65°C | 37°C | A | pBR322 DNA | 1, b | |||
Hpy99I | |
CGWCG/ | rCutSmart™ Buffer | 50 | 10 | <10 | 100 | 65°C | 37°C | A | λ DNA | ||||
Hpy166II | |
GTN/NAC | rCutSmart™ Buffer | 100 | 100 | 50 | 100 | 65°C | 37°C | C | pBR322 DNA | ||||
Hpy188III | |
TC/NNGA | rCutSmart™ Buffer | 100 | 100 | 10 | 100 | 65°C | 37°C | B | pUC19 DNA | 3, b | |||
I-CeuI | |
TAACTATAACGGTCCTAAGGTAGCGAA(-9/-13) | rCutSmart™ Buffer | 10 | 10 | 10 | 100 | 65°C | 37°C | B | pBHS ScaI-linearized Control Plasmid | ||||
I-SceI | |
TAGGGATAACAGGGTAAT(-9/-13) | rCutSmart™ Buffer | 10 | 50 | 25 | 100 | 65°C | 37°C | B | pGPS2 NotI-linearized Control Plasmid | ||||
KasI | |
G/GCGCC | rCutSmart™ Buffer | 50 | 100 | 50 | 100 | 65°C | 37°C | B | pBR322 DNA | 3 | |||
KpnI-HF® | |
GGTAC/C | rCutSmart™ Buffer | 100 | 25 | <10 | 100 | No | 37°C | A | pXba DNA | ||||
MboI | |
/GATC | rCutSmart™ Buffer | 75 | 100 | 100 | 100 | 65°C | 37°C | A | λ DNA (dam-) | ||||
MboII | |
GAAGA(8/7) | rCutSmart™ Buffer | 100* | 100 | 50 | 100 | 65°C | 37°C | C | λ DNA (dam-) | b | |||
MfeI-HF® | |
C/AATTG | rCutSmart™ Buffer | 75 | 25 | <10 | 100 | No | 37°C | A | λ DNA | ||||
MluCI | |
/AATT | rCutSmart™ Buffer | 100 | 10 | 10 | 100 | No | 37°C | A | λ DNA | ||||
MluI-HF® | |
A/CGCGT | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | No | 37°C | A | λ DNA | ||||
MlyI | |
GAGTC(5/5) | rCutSmart™ Buffer | 50 | 50 | 10 | 100 | 65°C | 37°C | A | λ DNA | b, d | |||
MmeI | |
TCCRAC(20/18) | rCutSmart™ Buffer | 50 | 100 | 50 | 100 | 65°C | 37°C | B | ΦX174 RF I DNA | b, c | |||
MnlI | |
CCTC(7/6) | rCutSmart™ Buffer | 75 | 100 | 50 | 100 | 65°C | 37°C | B | λ DNA | b | |||
MscI | |
TGG/CCA | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | 80°C | 37°C | C | λ DNA | ||||
MseI | |
T/TAA | rCutSmart™ Buffer | 75 | 100 | 75 | 100 | 65°C | 37°C | A | λ DNA | ||||
MslI | |
CAYNN/NNRTG | rCutSmart™ Buffer | 50 | 50 | <10 | 100 | 80°C | 37°C | A | λ DNA | ||||
MspA1I | |
CMG/CKG | rCutSmart™ Buffer | 10 | 50 | 10 | 100 | 65°C | 37°C | B | λ DNA | ||||
MspI | |
C/CGG | rCutSmart™ Buffer | 75 | 100 | 50 | 100 | No | 37°C | A | λ DNA | ||||
MspJI | |
CNNR(9/13) | rCutSmart™ Buffer + Activator | <10 | <10 | <10 | 100 | 65°C | 37°C | B | pBR322 (dcm+) DNA | 1, e | |||
MwoI | |
GCNNNNN/NNGC | rCutSmart™ Buffer | <10 | 100 | 100 | 100 | No | 60°C | B | λ DNA | ||||
NaeI | |
GCC/GGC | rCutSmart™ Buffer | 25 | 25 | <10 | 100 | No | 37°C | A | pXba DNA | b | |||
NarI | |
GG/CGCC | rCutSmart™ Buffer | 100 | 100 | 10 | 100 | 65°C | 37°C | A | pXba DNA | ||||
Nb.BbvCI | |
CCTCAGC(none/-2) | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | 80°C | 37°C | A | supercoiled plasmid DNA | e | |||
Nb.BsmI | |
GAATGC(none/-1) | NEBuffer™ r3.1 | <10 | 50 | 100 | 10 | 80°C | 65°C | A | supercoiled plasmid pBR322 DNA | e | |||
Nb.BsrDI | |
GCAATG(none/0) | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | 80°C | 37°C | A | supercoiled pUC19 DNA | e | |||
Nb.BssSI | |
CACGAG(none/-1) | NEBuffer™ r3.1 | 10 | 100 | 100 | 25 | No | 37°C | B | supercoiled pUC19 DNA | e | |||
Nb.BtsI | |
GCAGTG(none/0) | rCutSmart™ Buffer | 75 | 100 | 75 | 100 | 80°C | 37°C | A | supercoiled pUC101 DNA (dam-/dcm-) | e | |||
NciI | |
CC/SGG | rCutSmart™ Buffer | 100 | 25 | 10 | 100 | No | 37°C | A | λ DNA | b | |||
NcoI § | |
C/CATGG | NEBuffer™ r3.1 | 100 | 100 | 100 | 100+ | 80°C | 37°C | A | λ DNA | ||||
NcoI-HF® | |
C/CATGG | rCutSmart™ Buffer | 50 | 100 | 10 | 100 | 80°C | 37°C | B | λ DNA | ||||
NdeI | |
CA/TATG | rCutSmart™ Buffer | 75 | 100 | 100 | 100 | 65°C | 37°C | A | λ DNA | ||||
NgoMIV | |
G/CCGGC | rCutSmart™ Buffer | 100 | 50 | 10 | 100 | No | 37°C | A | pXba DNA | 1 | |||
NheI-HF® | |
G/CTAGC | rCutSmart™ Buffer | 100 | 25 | 10 | 100 | 80°C | 37°C | C | λ DNA (HindIII digest) | ||||
NlaIII | |
CATG/ | rCutSmart™ Buffer | <10 | <10 | <10 | 100 | 65°C | 37°C | B | ΦX174 RF I DNA | ||||
NlaIV | |
GGN/NCC | rCutSmart™ Buffer | 10 | 10 | 10 | 100 | 65°C | 37°C | B | pBR322 DNA | ||||
NmeAIII | |
GCCGAG(21/19) | rCutSmart™ Buffer | 10 | 10 | <10 | 100 | 65°C | 37°C | B | ΦX174 RF I DNA | c | |||
NotI § | |
GC/GGCCGC | NEBuffer™ r3.1 | <10 | 50 | 100 | 25 | 65°C | 37°C | C | pBC4 DNA | ||||
NotI-HF® | |
GC/GGCCGC | rCutSmart™ Buffer | 25 | 100 | 25 | 100 | 65°C | 37°C | A | pBC4 DNA | ||||
NruI-HF® | |
TCG/CGA | rCutSmart™ Buffer | 0 | 25 | 50 | 100 | No | 37°C | A | λ DNA | ||||
NsiI § | |
ATGCA/T | NEBuffer™ r3.1 | 10 | 75 | 100 | 25 | 65°C | 37°C | B | λ DNA | ||||
NsiI-HF® | |
ATGCA/T | rCutSmart™ Buffer | <10 | 20 | <10 | 100 | 80°C | 37°C | B | λ DNA | ||||
NspI | |
RCATG/Y | rCutSmart™ Buffer | 100 | 100 | <10 | 100 | 65°C | 37°C | A | λ DNA | ||||
Nt.AlwI | |
GGATC(4/none) | rCutSmart™ Buffer | 10 | 100 | 100 | 100 | 80°C | 37°C | A | pUC101 DNA (dam-/dcm-) | e | |||
Nt.BbvCI | |
CCTCAGC(-5/none) | rCutSmart™ Buffer | 50 | 100 | 10 | 100 | 80°C | 37°C | A | supercoiled plasmid DNA | e | |||
Nt.BsmAI | |
GTCTC(1/none) | rCutSmart™ Buffer | 100 | 50 | 10 | 100 | 65°C | 37°C | A | supercoiled plasmid DNA | e | |||
Nt.BspQI | |
GCTCTTC(1/none) | NEBuffer™ r3.1 | <10 | 25 | 100 | 10 | 80°C | 50°C | B | supercoiled pUC19 DNA | e | |||
Nt.BstNBI | |
GAGTC(4/none) | NEBuffer™ r3.1 | 0 | 10 | 100 | 10 | 80°C | 55°C | A | T7 DNA | e | |||
Nt.CviPII | |
CCD(-3/none) | rCutSmart™ Buffer | 10 | 100 | 25 | 100 | 65°C | 37°C | A | pUC19 DNA | e | |||
PacI | |
TTAAT/TAA | rCutSmart™ Buffer | 100 | 75 | 10 | 100 | 65°C | 37°C | A | pNEB193 DNA | ||||
PaeR7I | |
C/TCGAG | rCutSmart™ Buffer | 25 | 100 | 10 | 100 | No | 37°C | A | λ DNA (HindIII digest) | ||||
PaqCI | |
CACCTGC(4/8) | rCutSmart™ Buffer + Activator | 10 | 100 | 10 | 100 | 65°C | 37°C | B | λ DNA | 1 | |||
PciI | |
A/CATGT | NEBuffer™ r3.1 | 50 | 75 | 100 | 50* | 80°C | 37°C | B | pXba DNA | ||||
PflFI | |
GACN/NNGTC | rCutSmart™ Buffer | 25 | 100 | 25 | 100 | 65°C | 37°C | A | pBC4 DNA | b | |||
PflMI | |
CCANNNN/NTGG | NEBuffer™ r3.1 | 0 | 100 | 100 | 50 | 65°C | 37°C | A | λ DNA | 3, b, d | |||
PI-PspI | |
TGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17) | NEBuffer™ PI-PspI + rAlbumin | 10 | 10 | 10 | 10 | No | 65°C | B | pAKR7 XmnI-linearized Control Plasmid | ||||
PI-SceI | |
ATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19) | NEBuffer™ PI-SceI + rAlbumin | 10 | 10 | 10 | 10 | 65°C | 37°C | B | pBSvdeX XmnI-linearized Control Plasmid | ||||
PleI | |
GAGTC(4/5) | rCutSmart™ Buffer | 25 | 50 | 25 | 100 | 65°C | 37°C | A | λ DNA | b, d | |||
PluTI | |
GGCGC/C | rCutSmart™ Buffer | 100 | 25 | <10 | 100 | 65°C | 37°C | A | pXba DNA | b | |||
PmeI | |
GTTT/AAAC | rCutSmart™ Buffer | <10 | 50 | 10 | 100 | 65°C | 37°C | A | λ DNA | ||||
PmlI | |
CAC/GTG | rCutSmart™ Buffer | 100 | 50 | <10 | 100 | 65°C | 37°C | A | λ DNA (HindIII digest) DNA | ||||
PpuMI | |
RG/GWCCY | rCutSmart™ Buffer | <10 | <10 | <10 | 100 | No | 37°C | B | λ DNA (HindIII digest) | ||||
PshAI | |
GACNN/NNGTC | rCutSmart™ Buffer | 25 | 50 | 10 | 100 | 65°C | 37°C | A | λ DNA | ||||
PsiI-v2 | |
TTA/TAA | rCutSmart™ Buffer | 25 | 50 | 10 | 100 | 65°C | 37°C | B | λ DNA | 3 | |||
PspGI | |
/CCWGG | rCutSmart™ Buffer | 25 | 100 | 50 | 100 | No | 75°C | A | T7 DNA | 3 | |||
PspOMI | |
G/GGCCC | rCutSmart™ Buffer | 10 | 10 | <10 | 100 | 65°C | 37°C | B | pXba DNA | ||||
PspXI | |
VC/TCGAGB | rCutSmart™ Buffer | <10 | 100 | 25 | 100 | No | 37°C | B | λ DNA (HindIII digest) | ||||
PstI § | |
CTGCA/G | NEBuffer™ r3.1 | 75 | 75 | 100 | 50* | 80°C | 37°C | C | λ DNA | ||||
PstI-HF® | |
CTGCA/G | rCutSmart™ Buffer | 10 | 75 | 50 | 100 | No | 37°C | C | λ DNA | ||||
PvuI-HF® | |
CGAT/CG | rCutSmart™ Buffer | 25 | 100 | 100 | 100 | No | 37°C | B | pXba DNA | ||||
PvuII § | |
CAG/CTG | NEBuffer™ r3.1 | 50 | 100 | 100 | 100* | No | 37°C | B | λ DNA | ||||
PvuII-HF® | |
CAG/CTG | rCutSmart™ Buffer | <10 | <10 | <10 | 100 | No | 37°C | B | λ DNA | ||||
RsaI | |
GT/AC | rCutSmart™ Buffer | 25 | 50 | <10 | 100 | No | 37°C | A | λ DNA | ||||
RsrII | |
CG/GWCCG | rCutSmart™ Buffer | 25 | 75 | 10 | 100 | 65°C | 37°C | C | λ DNA | ||||
SacI-HF® | |
GAGCT/C | rCutSmart™ Buffer | 10 | 50 | <10 | 100 | 65°C | 37°C | A | λ DNA (HindIII digest) | ||||
SacII | |
CCGC/GG | rCutSmart™ Buffer | 10 | 100 | 10 | 100 | 65°C | 37°C | A | pXba DNA | ||||
SalI § | |
G/TCGAC | NEBuffer™ r3.1 | <10 | <10 | 100 | <10 | 65°C | 37°C | A | λ DNA (HindIII digest) | ||||
SalI-HF® | |
G/TCGAC | rCutSmart™ Buffer | 10 | 100 | 100 | 100 | 65°C | 37°C | A | λ DNA (HindIII digest) | ||||
SapI | |
GCTCTTC(1/4) | rCutSmart™ Buffer | 75 | 50 | <10 | 100 | 65°C | 37°C | B | λ DNA | ||||
Sau3AI | |
/GATC | NEBuffer™ r1.1 | 100 | 50 | 10 | 100+ | 65°C | 37°C | A | λ DNA | b | |||
Sau96I | |
G/GNCC | rCutSmart™ Buffer | 50 | 100 | 100 | 100 | 65°C | 37°C | A | λ DNA | ||||
SbfI-HF® | |
CCTGCA/GG | rCutSmart™ Buffer | 50 | 25 | <10 | 100 | 80°C | 37°C | B | λ DNA | ||||
ScaI-HF® | |
AGT/ACT | rCutSmart™ Buffer | 100 | 100 | 10 | 100 | 80°C | 37°C | B | λ DNA | ||||
ScrFI | |
CC/NGG | rCutSmart™ Buffer | 100 | 100 | 100 | 100 | 65°C | 37°C | C | λ DNA | 2, a | |||
SexAI | |
A/CCWGGT | rCutSmart™ Buffer | 100 | 75 | 50 | 100 | 65°C | 37°C | A | pBC4 DNA (dcm-) | 3, b, d | |||
SfaNI | |
GCATC(5/9) | NEBuffer™ r3.1 | <10 | 75 | 100 | 25 | 65°C | 37°C | B | ΦX174 RF I DNA | 3, b | |||
SfcI | |
C/TRYAG | rCutSmart™ Buffer | 75 | 50 | 25 | 100 | 65°C | 37°C | B | λ DNA | 3 | |||
SfiI | |
GGCCNNNN/NGGCC | rCutSmart™ Buffer | 25 | 100 | 50 | 100 | No | 50°C | C | pXba DNA | ||||
SfoI | |
GGC/GCC | rCutSmart™ Buffer | 50 | 100 | 100 | 100 | No | 37°C | B | λ DNA (HindIII digest) | ||||
SgrAI | |
CR/CCGGYG | rCutSmart™ Buffer | 100 | 100 | 10 | 100 | 65°C | 37°C | A | λ DNA | 1 | |||
SmaI | |
CCC/GGG | rCutSmart™ Buffer | <10 | <10 | <10 | 100 | 65°C | 37°C | B | λ DNA (HindIII digest) | b | |||
SmlI | |
C/TYRAG | rCutSmart™ Buffer | 25 | 75 | 25 | 100 | No | 55°C | A | λ DNA | b | |||
SnaBI | |
TAC/GTA | rCutSmart™ Buffer | 50* | 50 | 10 | 100 | 80°C | 37°C | A | T7 DNA | 1 | |||
SpeI-HF® | |
A/CTAGT | rCutSmart™ Buffer | 25 | 50 | 10 | 100 | 80°C | 37°C | C | pXba-XbaI DNA | ||||
SphI § | |
GCATG/C | NEBuffer™ r2.1 | 100 | 100 | 50 | 100+ | 65°C | 37°C | B | λ DNA | 2 | |||
SphI-HF® | |
GCATG/C | rCutSmart™ Buffer | 50 | 25 | 10 | 100 | 65°C | 37°C | B | λ DNA | ||||
SrfI | |
GCCC/GGGC | rCutSmart™ Buffer | 10 | 50 | 0 | 100 | 65°C | 37°C | B | pNEB193-SrfI DNA | ||||
SspI-HF® | |
AAT/ATT | rCutSmart™ Buffer | 25 | 100 | <10 | 100 | 65°C | 37°C | B | λ DNA | ||||
StuI | |
AGG/CCT | rCutSmart™ Buffer | 50 | 100 | 50 | 100 | No | 37°C | A | λ DNA | ||||
StyD4I | |
/CCNGG | rCutSmart™ Buffer | 10 | 100 | 100 | 100 | 65°C | 37°C | B | λ DNA | ||||
StyI-HF® | |
C/CWWGG | rCutSmart™ Buffer | 25 | 100 | 25 | 100 | 65°C | 37°C | A | λ DNA | ||||
SwaI | |
ATTT/AAAT | NEBuffer™ r3.1 | 10 | 10 | 100 | 10 | 65°C | 25°C | B | pXba DNA | b, d | |||
TaqI-v2 | |
T/CGA | rCutSmart™ Buffer | 50 | 100 | 50 | 100 | No | 65°C | B | λ DNA | ||||
TfiI | |
G/AWTC | rCutSmart™ Buffer | 50 | 100 | 100 | 100 | No | 65°C | C | λ DNA | ||||
TseI | |
G/CWGC | rCutSmart™ Buffer | 75 | 100 | 100 | 100 | No | 65°C | B | λ DNA | 3 | |||
Tsp45I | |
/GTSAC | rCutSmart™ Buffer | 100 | 50 | <10 | 100 | No | 65°C | A | λ DNA | ||||
TspMI | |
C/CCGGG | rCutSmart™ Buffer | 50* | 75* | 50* | 100 | No | 75°C | B | pBC4 DNA | d | |||
TspRI | |
NNCASTGNN/ | rCutSmart™ Buffer | 25 | 50 | 25 | 100 | No | 65°C | B | λ DNA | ||||
Tth111I | |
GACN/NNGTC | rCutSmart™ Buffer | 25 | 100 | 25 | 100 | No | 65°C | B | pBC4 DNA | b | |||
WarmStart® Nt.BstNBI | |
GAGTC(4/none) | NEBuffer™ r3.1 | 0 | 10 | 100 | 25 | 80°C | 55°C | A | T7 DNA | ||||
XbaI | |
T/CTAGA | rCutSmart™ Buffer | <10 | 100 | 75 | 100 | 65°C | 37°C | A | λ DNA (dam-/Hind III digest) | ||||
XcmI | |
CCANNNNN/NNNNTGG | NEBuffer™ r2.1 | 10 | 100 | 25 | 100* | 65°C | 37°C | C | λ DNA | 2 | |||
XhoI | |
C/TCGAG | rCutSmart™ Buffer | 75 | 100 | 100 | 100 | 65°C | 37°C | A | λ DNA (HindIII digest) | b | |||
XmaI | |
C/CCGGG | rCutSmart™ Buffer | 25 | 50 | <10 | 100 | 65°C | 37°C | A | pXba DNA | 3 | |||
XmnI | |
GAANN/NNTTC | rCutSmart™ Buffer | 50 | 75 | <10 | 100 | 65°C | 37°C | A | λ DNA | b | |||
ZraI | |
GAC/GTC | rCutSmart™ Buffer | 100 | 25 | 10 | 100 | 80°C | 37°C | B | λ DNA |
§ An HF version of this enzyme is available.
Ligation and Recutting Notes
The following notes appear with any enzymes having ligation efficiencies lower than 100% as assessed by ligation and recutting.
a. Ligation is less than 10%.
b. Ligation is 25% -75%.
c. Recutting after ligation is less than 5%.
d. Recutting after ligation is 50% -75%.
e. Ligation and recutting after ligation is not applicable since the enzyme is either a nicking enzyme, is affected by methylation, or if the enzyme cleaves outside its recognition sequence.
Star Activity Notes
The following notes appear with any enzymes when star activity is a concern.
1. Star Activity may result from extended digestion, high enzyme concentration or a glycerol concentration of > 5%.
2. Star Activity may result from extended digestion.
3. Star Activity may result from a glycerol concentration of > 5%.
*. May exhibit star activity in this buffer.
+. For added flexibility, NEB offers an isoschizomer or HF enzyme, supplied with CutSmart Buffer.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.