NEBridge® Golden Gate Assembly Kit (BsmBI-v2)

Complement this kit with NEBridge Golden Gate Assembly Kit (BsaI-HFv2) (NEB #E1601)

Learn about ligase fidelity and push the limits of your Golden Gate Assembly Reactions (50+ fragments)

  • Uses BsmBI-v2, which is optimized for Golden Gate Assembly
  • Use for seamless cloning – no scar remains following assembly
  • Ideal for ordered assembly of multiple fragments (2-50+) in a single reaction
  • Can also be used for cloning of single inserts and library preparations
  • Efficient with regions with high GC content and areas of repeats
  • Compatible with a broad range of fragment sizes (< 100 bp to > 15 kb)
  • Includes destination plasmid compatible with a variety of Type IIS restriction enzymes commonly used in Golden Gate Assembly
  • Free tool available at
  • Try our free Ligase Fidelity Tools ( for the design of high-fidelity Golden Gate Assemblies)

We're sorry, this butterfly has already been found. Learn more about how NEB is celebrating our 50th anniversary.

Catalog # Concentration Size List Price Your Price Quantity
E1602S Not Applicable 20 reactions $171.00
E1602L Not Applicable 100 reactions $456.00
Please enter a quantity for at least one size
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