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Isoschizomers

Restriction endonucleases that recognize the same sequence are isoschizomers. The first example discovered is called a prototype and all subsequent enzymes that recognize the same sequence are isoschizomers of the prototype. The list below contains isoschizomers for commercially available restriction endonucleases. It also specifies which isoschizomer is available from New England Biolabs.

All recognition sequences are written 5´ to 3´ using the single letter code nomenclature with the point of cleavage indicated by a "/".

Numbers in parentheses indicate point of cleavage for non-palindromic enzymes. For example, GGTCTC(1/5) indicates cleavage at:
5´ ...GGTCTCN/...3´
3´ ...CCAGAGNNNNN/...5´

Isoschizomers with alternative cleavage sites are indicated with a " ^ ". Enzymes that are not currently commercially available are indicated with a " x ".

Neoschizomers are a subset of isoschizomers that recognize the same sequence, but cleave at different positions from the prototype. Thus, AatII (recognition sequence: GACGT↓C) and ZraI (recognition sequence: GAC↓GTC) are neoschizomers of one another, while HpaII (recognition sequence: C↓CGG) and MspI (recognition sequence: C↓CGG) are isoschizomers. Analogous designations are not appropriate for MTases, where the differences between enzymes are not so easily defined and usually have not been well characterized.

Enzyme Finder tool can help you identify commercially available ischoschizomers.

Enzyme	Sequence	NEB Enzyme	Catalog Number	Cut Site	Other Isoschizomers
Enzyme	Sequence	NEB Enzyme	Catalog Number	Cut Site	Other Isoschizomers	AanI	TTA/TAA	PsiI-v2	R0744	TTA/TAA	PsiI-v2
AarI	CACCTGC(4/8)	PaqCI	R0745	CACCTGC(4/8)	PaqCI
AasI	GACNNNN/NNGTC	DrdI	R0530	GACNNNN/NNGTC	DrdI, DseDI
AatII	GACGT/C	AatII	R0117	GACGT/C	ZraI^
		ZraI^	R0659	GAC/GTC
AbaB8342IV x	CATTAG
AbaCIII x	CTATCAV
Aba13301I x	GCAAAC
Aba1056II x	CACRAC
Aba6411II x	CRRTAAG
AbaPBA3II x	CAYGAC
AbaUMB2I x	YCCGSS
Abr4036II x	GRTYGACC
AbsI	CC/TCGAGG
AccB7I	CCANNNN/NTGG	PflMI	R0509	CCANNNN/NTGG	PflMI, Van91I
AccB1I	G/GYRCC	BanI	R0118	G/GYRCC	BanI, BshNI, BspT107I
AccBSI	CCGCTC(-3/-3)	BsrBI	R0102	CCGCTC(-3/-3)	BsrBI, MbiI
Acc36I	ACCTGC(4/8)	BfuAI	R0701	ACCTGC(4/8)	BfuAI, BspMI, BveI
		BspMI	R0502	ACCTGC(4/8)
Acc16I	TGC/GCA	FspI	R0135	TGC/GCA	FspI, NsbI
Acc65I	G/GTACC	Acc65I	R0599	G/GTACC	Asp718I, KpnI^, KpnI-HF^
		KpnI^ ^§	R0142	GGTAC/C
		KpnI-HF^	R3142	GGTAC/C
AccI	GT/MKAC	AccI	R0161	GT/MKAC	FblI, XmiI
AccII	CG/CG	BstUI	R0518	CG/CG	Bsh1236I, BspFNI, BstFNI, BstUI, MvnI
AccIII	T/CCGGA	BspEI	R0540	T/CCGGA	Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I, MroI
AccIX x	GACRAC
Acc65V x	GACGCA
AccX x	GGARCA
AceIII x	CAGCTC(7/11)
AchA6III x	AGCCAG
AciI	CCGC(-3/-1)	AciI	R0551	CCGC(-3/-1)	BspACI, SsiI
AclI	AA/CGTT	AclI	R0598	AA/CGTT	Psp1406I
AclWI	GGATC(4/5)	AlwI	R0513	GGATC(4/5)	AlwI, BspPI
AcoI	Y/GGCCR	EaeI	R0508	Y/GGCCR	EaeI
Aco12261II x	CCRGAG
AcoY31II x	TAGCRAB
AcsI	R/AATTY	ApoI-HF	R3566	R/AATTY	ApoI-HF, XapI
AcuI	CTGAAG(16/14)	AcuI	R0641	CTGAAG(16/14)	Eco57I
AcvI	CAC/GTG	PmlI	R0532	CAC/GTG	BbrPI, Eco72I, PmaCI, PmlI, PspCI
AcyI	GR/CGYC	BsaHI	R0556	GR/CGYC	BsaHI, BssNI, BstACI, Hin1I, Hsp92I
AdeI	CACNNN/GTG	DraIII-HF	R3510	CACNNN/GTG	DraIII-HF
AfaI	GT/AC	CviQI^	R0639	G/TAC	Csp6I^, CviQI^, RsaI, RsaNI^
		RsaI	R0167	GT/AC
AfeI	AGC/GCT	AfeI	R0652	AGC/GCT	Aor51HI, Eco47III
AfiI	CCNNNNN/NNGG	BslI	R0555	CCNNNNN/NNGG	Bsc4I, BseLI, BslI
AflII	C/TTAAG	AflII	R0520	C/TTAAG	BfrI, BspTI, BstAFI, MspCI, Vha464I
AflIII	A/CRYGT	AflIII	R0541	A/CRYGT
AgeI	A/CCGGT	AgeI ^§	R0552	A/CCGGT	AgeI-HF, AsiGI, BshTI, CspAI, PinAI
		AgeI-HF	R3552	A/CCGGT
AgeI-HF	A/CCGGT	AgeI ^§	R0552	A/CCGGT	AgeI, AsiGI, BshTI, CspAI, PinAI
		AgeI-HF	R3552	A/CCGGT
AgsI	TTS/AA
Aha10306III x	GTAGAT
AhaIII x	TTT/AAA	DraI	R0129	TTT/AAA	DraI
AhdI	GACNNN/NNGTC	AhdI	R0584	GACNNN/NNGTC	BmeRI, DriI, Eam1105I
AhlI	A/CTAGT	SpeI-HF	R3133	A/CTAGT	BcuI, SpeI-HF
AhyRBAHI x	GCYYGAC
AhyYL17I x	YAAMGAG
AjiI	CACGTC(-3/-3)	BmgBI	R0628	CACGTC(-3/-3)	BmgBI, BtrI
AjnI	/CCWGG	BstNI^	R0168	CC/WGG	BciT130I^, BseBI^, BstNI^, Bst2UI^, EcoRII, MvaI^, Psp6I, PspGI
		PspGI	R0611	/CCWGG
AjuI	(7/12)GAANNNNNNNTTGG(11/6)
AleI-v2	CACNN/NNGTG	AleI-v2	R0685	CACNN/NNGTG	OliI
AlfI x	(10/12)GCANNNNNNTGC(12/10)
AloI	(7/12)GAACNNNNNNTCC(12/7)
AluBI	AG/CT	AluI	R0137	AG/CT	AluI
AluI	AG/CT	AluI	R0137	AG/CT	AluBI
AlwFI x	GAAAYNNNNNRTG
Alw21I	GWGCW/C	BsiHKAI	R0570	GWGCW/C	Bbv12I, BsiHKAI
Alw26I	GTCTC(1/5)	BcoDI	R0542	GTCTC(1/5)	BcoDI, BsmAI, BstMAI
		BsmAI	R0529	GTCTC(1/5)
AlwI	GGATC(4/5)	AlwI	R0513	GGATC(4/5)	AclWI, BspPI
Alw44I	G/TGCAC	ApaLI	R0507	G/TGCAC	ApaLI, VneI
AlwNI	CAGNNN/CTG	AlwNI	R0514	CAGNNN/CTG	CaiI, PstNI
AmaCSI x	GCTCCA(11/9)
Ama87I	C/YCGRG	AvaI	R0152	C/YCGRG	AvaI, BmeT110I, BsiHKCI, BsoBI, Eco88I
		BsoBI	R0586	C/YCGRG
Aod1I x	GATCNAC
Aor51HI	AGC/GCT	AfeI	R0652	AGC/GCT	AfeI, Eco47III
Aor13HI	T/CCGGA	BspEI	R0540	T/CCGGA	AccIII, BseAI, Bsp13I, BspEI, Kpn2I, MroI
AoxI	/GGCC
ApaBI x	GCANNNNN/TGC	BstAPI^	R0654	GCANNNN/NTGC	BstAPI^
ApaI	GGGCC/C	ApaI	R0114	GGGCC/C	Bsp120I^, PspOMI^
		PspOMI^	R0653	G/GGCCC
ApaLI	G/TGCAC	ApaLI	R0507	G/TGCAC	Alw44I, VneI
ApeKI	G/CWGC	ApeKI	R0643	G/CWGC	TseI
		TseI	R0591	G/CWGC
ApoI-HF	R/AATTY	ApoI-HF	R3566	R/AATTY	AcsI, XapI
ApyPI x	ATCGAC(20/18)
AquII x	GCCGNAC(20/18)
AquIV x	GRGGAAG(19/17)
ArsI	(8/13)GACNNNNNNTTYG(11/6)
AscI	GG/CGCGCC	AscI	R0558	GG/CGCGCC	PalAI, SgsI
AseI	AT/TAAT	AseI	R0526	AT/TAAT	PshBI, VspI
AsiGI	A/CCGGT	AgeI ^§	R0552	A/CCGGT	AgeI, AgeI-HF, BshTI, CspAI, PinAI
		AgeI-HF	R3552	A/CCGGT
AsiSI	GCGAT/CGC	AsiSI	R0630	GCGAT/CGC	RgaI, SfaAI, SgfI
Asl11923II x	GGGABCC
AspA2I	C/CTAGG	AvrII	R0174	C/CTAGG	AvrII, BlnI, XmaJI
AspAMDIV x	ACCCAC
AspBHI x	YSCNS(8/12)
AspDUT2V x	GNGCAAC
Asp337I x	CARABGG
Asp103I x	CGRAGGC
Asp718I	G/GTACC	Acc65I	R0599	G/GTACC	Acc65I, KpnI^, KpnI-HF^
		KpnI^ ^§	R0142	GGTAC/C
		KpnI-HF^	R3142	GGTAC/C
Asp700I	GAANN/NNTTC	XmnI	R0194	GAANN/NNTTC	MroXI, PdmI, XmnI
AspJHL3II x	CGCCCAG
AspLEI	GCG/C	HhaI	R0139	GCG/C	BstHHI, CfoI, HhaI, Hin6I^, HinP1I^, HspAI^
		HinP1I^	R0124	G/CGC
AspNIH4III x	AAGAACB
AspS9I	G/GNCC	Sau96I	R0165	G/GNCC	BmgT120I, Cfr13I, PspPI, Sau96I
AspSLV7III x	GTCTCA
Asp114pII x	AGCABCC
AsuC2I	CC/SGG	NciI	R0196	CC/SGG	BcnI, BpuMI, NciI
AsuHPI	GGTGA(8/7)	HphI	R0158	GGTGA(8/7)	HphI
AsuI x	G/GNCC	Sau96I	R0165	G/GNCC	AspS9I, BmgT120I, Cfr13I, PspPI, Sau96I
AsuII	TT/CGAA	BstBI	R0519	TT/CGAA	Bpu14I, Bsp119I, BspT104I, BstBI, NspV, SfuI
Asu14238IV x	CGTRAC
AsuNHI	G/CTAGC	BmtI-HF^	R3658	GCTAG/C	BmtI-HF^, BspOI^, NheI-HFv2
		NheI-HF	R3131	G/CTAGC
		NheI-HFv2	R3131	G/CTAGC
AteTI x	GGGRAG
AvaI	C/YCGRG	AvaI	R0152	C/YCGRG	Ama87I, BmeT110I, BsiHKCI, BsoBI, Eco88I
		BsoBI	R0586	C/YCGRG
AvaII	G/GWCC	AvaII	R0153	G/GWCC	Bme18I, Eco47I, SinI, VpaK11BI
AvaIII x	ATGCAT	NsiI^§	R0127	ATGCA/T	EcoT22I, Mph1103I, NsiI, NsiI-HF, Zsp2I
		NsiI-HF	R3127	ATGCA/T
Avi249I x	CTGCA
AvrII	C/CTAGG	AvrII	R0174	C/CTAGG	AspA2I, BlnI, XmaJI
Awo1030IV x	GCCRAG
AxyI	CC/TNAGG	Bsu36I	R0524	CC/TNAGG	Bse21I, Bsu36I, Eco81I
BaeGI	GKGCM/C	BaeGI	R0708	GKGCM/C	BseSI, BstSLI
BaeI	(10/15)ACNNNNGTAYC(12/7)	BaeI	R0613	(10/15)ACNNNNGTAYC(12/7)
Bag18758I x	CCCGAG
BalI	TGG/CCA	MscI	R0534	TGG/CCA	MlsI, MluNI, Mox20I, MscI, Msp20I
BamHI	G/GATCC	BamHI^§	R0136	G/GATCC	BamHI-HF
		BamHI-HF	R3136	G/GATCC
BamHI-HF	G/GATCC	BamHI^§	R0136	G/GATCC	BamHI
		BamHI-HF	R3136	G/GATCC
BanI	G/GYRCC	BanI	R0118	G/GYRCC	AccB1I, BshNI, BspT107I
BanII	GRGCY/C	BanII	R0119	GRGCY/C	Eco24I, EcoT38I, FriOI
BanLI x	RTCAGG
BarI	(7/12)GAAGNNNNNNTAC(12/7)
BauI	CACGAG(-5/-1)	BssSI	R0680	CACGAG(-5/-1)	BssSI-v2, Bst2BI
		BssSI-v2	R0680	CACGAG(-5/-1)
Bau1417V x	GTTCAG
Bbr52II x	GGCGAG
Bbr7017II x	CGGGAG
Bbr7017III x	GGRCAG
Bbr57III x	GTRAAYG
BbrPI	CAC/GTG	PmlI	R0532	CAC/GTG	AcvI, Eco72I, PmaCI, PmlI, PspCI
BbsI	GAAGAC(2/6)	BbsI^§	R0539	GAAGAC(2/6)	BbsI-HF, BpiI, BstV2I
		BbsI-HF	R3539	GAAGAC(2/6)
BbsI-HF	GAAGAC(2/6)	BbsI^§	R0539	GAAGAC(2/6)	BbsI, BpiI, BstV2I
		BbsI-HF	R3539	GAAGAC(2/6)
BbuB31I x	GNAAYG
BbuB31II x	CGRKA
BbvCI	CCTCAGC(-5/-2)	BbvCI	R0601	CCTCAGC(-5/-2)
BbvI	GCAGC(8/12)	BbvI	R0173	GCAGC(8/12)	BseXI, BstV1I, Lsp1109I
Bbv12I	GWGCW/C	BsiHKAI	R0570	GWGCW/C	Alw21I, BsiHKAI
BbvII x	GAAGAC(2/6)	BbsI^§	R0539	GAAGAC(2/6)	BbsI, BbsI-HF, BpiI, BstV2I
		BbsI-HF	R3539	GAAGAC(2/6)
BccI	CCATC(4/5)	BccI	R0704	CCATC(4/5)
BceAI	ACGGC(12/14)	BceAI	R0623	ACGGC(12/14)
Bce3081I x	TAGGAG
Bce83I x	CTTGAG(16/14)	BpuEI	R0633	CTTGAG(16/14)	BpuEI
Bce10661III x	TATCNAG
BcefI x	ACGGC(12/13)	BceAI^	R0623	ACGGC(12/14)	BceAI^
BcgI	(10/12)CGANNNNNNTGC(12/10)	BcgI	R0545	(10/12)CGANNNNNNTGC(12/10)
BciT130I	CC/WGG	BstNI	R0168	CC/WGG	AjnI^, BseBI, BstNI, Bst2UI, EcoRII^, MvaI, Psp6I^, PspGI^
		PspGI^	R0611	/CCWGG
BciVI	GTATCC(6/5)	BciVI	R0596	GTATCC(6/5)	BfuI, BsuI
BclI	T/GATCA	BclI^§	R0160	T/GATCA	BclI-HF, FbaI, Ksp22I
		BclI-HF	R3160	T/GATCA
BclI-HF	T/GATCA	BclI^§	R0160	T/GATCA	BclI, FbaI, Ksp22I
		BclI-HF	R3160	T/GATCA
BcnI	CC/SGG	NciI	R0196	CC/SGG	AsuC2I, BpuMI, NciI
BcoDI	GTCTC(1/5)	BcoDI	R0542	GTCTC(1/5)	Alw26I, BsmAI, BstMAI
		BsmAI	R0529	GTCTC(1/5)
Bco11035III x	GAAGCY
BcuI	A/CTAGT	SpeI-HF	R3133	A/CTAGT	AhlI, SpeI-HF
BdaI x	(10/12)TGANNNNNNTCA(12/10)
BetI x	W/CCGGW	BsaWI	R0567	W/CCGGW	BsaWI
BfaI	C/TAG	BfaI	R0568	C/TAG	FspBI, MaeI, SspMI, XspI
BfaSII x	GANGGAG
BfiI x	ACTGGG(5/4)	BmrI	R0600	ACTGGG(5/4)	BmrI, BmuI
BfmI	C/TRYAG	SfcI	R0561	C/TRYAG	BstSFI, SfcI
BfoI	RGCGC/Y	HaeII	R0107	RGCGC/Y	BstH2I, HaeII
BfrI	C/TTAAG	AflII	R0520	C/TTAAG	AflII, BspTI, BstAFI, MspCI, Vha464I
BfuAI	ACCTGC(4/8)	BfuAI	R0701	ACCTGC(4/8)	Acc36I, BspMI, BveI
		BspMI	R0502	ACCTGC(4/8)
BfuI	GTATCC(6/5)	BciVI	R0596	GTATCC(6/5)	BciVI, BsuI
Bga514I x	GTRAAG
BglI	GCCNNNN/NGGC	BglI	R0143	GCCNNNN/NGGC
BglII	A/GATCT	BglII	R0144	A/GATCT
Bhe175II x	GCCCNA
BinI x	GGATC(4/5)	AlwI	R0513	GGATC(4/5)	AclWI, AlwI, BspPI
BisI	GC/NGC				BlsI^, GluI, PkrI^
BkrAM31DI x	RTTAAATM
Ble402II x	GRAGCAG
BlnI	C/CTAGG	AvrII	R0174	C/CTAGG	AspA2I, AvrII, XmaJI
BloAII x	GAGGAC
BlpI	GC/TNAGC	BlpI	R0585	GC/TNAGC	Bpu1102I, Bsp1720I
BlsI	GCN/GC				BisI^, GluI^, PkrI
BmcAI	AGT/ACT	ScaI	R3122	AGT/ACT	ScaI-HF, ZrmI
		ScaI-HF	R3122	AGT/ACT
Bme18I	G/GWCC	AvaII	R0153	G/GWCC	AvaII, Eco47I, SinI, VpaK11BI
Bme1390I	CC/NGG	ScrFI	R0110	CC/NGG	BmrFI, BstSCI^, MspR9I, ScrFI, StyD4I^
		StyD4I^	R0638	/CCNGG
BmeRI	GACNNN/NNGTC	AhdI	R0584	GACNNN/NNGTC	AhdI, DriI, Eam1105I
BmeT110I	C/YCGRG	AvaI	R0152	C/YCGRG	Ama87I, AvaI, BsiHKCI, BsoBI, Eco88I
		BsoBI	R0586	C/YCGRG
BmgBI	CACGTC(-3/-3)	BmgBI	R0628	CACGTC(-3/-3)	AjiI, BtrI
BmgI x	GKGCCC
BmgT120I	G/GNCC	Sau96I	R0165	G/GNCC	AspS9I, Cfr13I, PspPI, Sau96I
BmiI	GGN/NCC	NlaIV	R0126	GGN/NCC	BspLI, NlaIV, PspN4I
BmrFI	CC/NGG	ScrFI	R0110	CC/NGG	Bme1390I, BstSCI^, MspR9I, ScrFI, StyD4I^
		StyD4I^	R0638	/CCNGG
BmrI	ACTGGG(5/4)	BmrI	R0600	ACTGGG(5/4)	BmuI
BmsI	GCATC(5/9)	SfaNI	R0172	GCATC(5/9)	LweI, SfaNI
BmtI-HF	GCTAG/C	BmtI-HF^	R3658	GCTAG/C	AsuNHI^, BspOI, NheI-HFv2^
		BmtI-HF	R3658	GCTAG/C
		NheI-HF^	R3131	G/CTAGC
		NheI-HF	R3131	G/CTAGC
		NheI-HFv2^	R3131	G/CTAGC
BmuI	ACTGGG(5/4)	BmrI	R0600	ACTGGG(5/4)	BmrI
BoxI	GACNN/NNGTC	PshAI	R0593	GACNN/NNGTC	BstPAI, PshAI
BpiI	GAAGAC(2/6)	BbsI^§	R0539	GAAGAC(2/6)	BbsI, BbsI-HF, BstV2I
		BbsI-HF	R3539	GAAGAC(2/6)
BplI	(8/13)GAGNNNNNCTC(13/8)
BpmI	CTGGAG(16/14)	BpmI	R0565	CTGGAG(16/14)	GsuI
Bps6700III x	TACCNAG
BpuEI	CTTGAG(16/14)	BpuEI	R0633	CTTGAG(16/14)
Bpu14I	TT/CGAA	BstBI	R0519	TT/CGAA	AsuII, Bsp119I, BspT104I, BstBI, NspV, SfuI
Bpu1102I	GC/TNAGC	BlpI	R0585	GC/TNAGC	BlpI, Bsp1720I
Bpu10I	CCTNAGC(-5/-2)	Bpu10I	R0649	CCTNAGC(-5/-2)
BpuMI	CC/SGG	NciI	R0196	CC/SGG	AsuC2I, BcnI, NciI
BsaAI	YAC/GTR	BsaAI	R0531	YAC/GTR	BstBAI, Ppu21I
BsaBI	GATNN/NNATC	BsaBI	R0537	GATNN/NNATC	Bse8I, BseJI
BsaHI	GR/CGYC	BsaHI	R0556	GR/CGYC	AcyI, BssNI, BstACI, Hin1I, Hsp92I
Bsa29I	AT/CGAT	BspDI	R0557	AT/CGAT	BseCI, BshVI, BspDI, Bsu15I, BsuTUI, ClaI
		ClaI	R0197	AT/CGAT
BsaI-HFv2	GGTCTC(1/5)	BsaI-HFv2	R3733	GGTCTC(1/5)	Bso31I, BspTNI, Eco31I
BsaJI	C/CNNGG	BsaJI	R0536	C/CNNGG	BseDI, BssECI
BsaWI	W/CCGGW	BsaWI	R0567	W/CCGGW
BsaXI	(9/12)ACNNNNNCTCC(10/7)	BsaXI	R0609	(9/12)ACNNNNNCTCC(10/7)
BsbI x	CAACAC(21/19)
BscGI x	CCCGT
Bsc4I	CCNNNNN/NNGG	BslI	R0555	CCNNNNN/NNGG	AfiI, BseLI, BslI
BscXI x	GCAGGC(-1/-5)
BseAI	T/CCGGA	BspEI	R0540	T/CCGGA	AccIII, Aor13HI, Bsp13I, BspEI, Kpn2I, MroI
BseBI	CC/WGG	BstNI	R0168	CC/WGG	AjnI^, BciT130I, BstNI, Bst2UI, EcoRII^, MvaI, Psp6I^, PspGI^
		PspGI^	R0611	/CCWGG
BseCI	AT/CGAT	BspDI	R0557	AT/CGAT	Bsa29I, BshVI, BspDI, Bsu15I, BsuTUI, ClaI
		ClaI	R0197	AT/CGAT
BseDI	C/CNNGG	BsaJI	R0536	C/CNNGG	BsaJI, BssECI
Bse3DI	GCAATG(2/0)	BsrDI	R0574	GCAATG(2/0)	BseMI, BsrDI
BseGI	GGATG(2/0)	BtsCI	R0647	GGATG(2/0)	BstF5I, BtsCI, FokI^
		FokI^	R0109	GGATG(9/13)
Bse8I	GATNN/NNATC	BsaBI	R0537	GATNN/NNATC	BsaBI, BseJI
Bse21I	CC/TNAGG	Bsu36I	R0524	CC/TNAGG	AxyI, Bsu36I, Eco81I
Bse1I	ACTGG(1/-1)	BsrI	R0527	ACTGG(1/-1)	BseNI, BsrI
Bse118I	R/CCGGY	BsrFI	R0682	R/CCGGY	BsrFI-v2, BssAI, Cfr10I
		BsrFI-v2	R0682	R/CCGGY
BseJI	GATNN/NNATC	BsaBI	R0537	GATNN/NNATC	BsaBI, Bse8I
BseLI	CCNNNNN/NNGG	BslI	R0555	CCNNNNN/NNGG	AfiI, Bsc4I, BslI
BseMI	GCAATG(2/0)	BsrDI	R0574	GCAATG(2/0)	Bse3DI, BsrDI
BseMII	CTCAG(10/8)	BspCNI^	R0624	CTCAG(9/7)	BspCNI^
BseNI	ACTGG(1/-1)	BsrI	R0527	ACTGG(1/-1)	Bse1I, BsrI
BsePI	G/CGCGC	BssHII	R0199	G/CGCGC	BssHII, PauI, PteI
BseRI	GAGGAG(10/8)	BseRI	R0581	GAGGAG(10/8)
BseSI	GKGCM/C	BaeGI	R0708	GKGCM/C	BaeGI, BstSLI
BseX3I	C/GGCCG	EagI-HF	R3505	C/GGCCG	BstZI, EagI-HFv2, EclXI, Eco52I
		EagI-HFv2	R3505	C/GGCCG
BseXI	GCAGC(8/12)	BbvI	R0173	GCAGC(8/12)	BbvI, BstV1I, Lsp1109I
BseYI	CCCAGC(-5/-1)	BseYI	R0635	CCCAGC(-5/-1)	GsaI^, PspFI
BsgI	GTGCAG(16/14)	BsgI	R0559	GTGCAG(16/14)
BshFI	GG/CC	HaeIII	R0108	GG/CC	BsnI, BspANI, BsuRI, HaeIII
Bsh1285I	CGRY/CG	BsiEI	R0554	CGRY/CG	BsiEI, BstMCI
Bsh1236I	CG/CG	BstUI	R0518	CG/CG	AccII, BspFNI, BstFNI, BstUI, MvnI
BshNI	G/GYRCC	BanI	R0118	G/GYRCC	AccB1I, BanI, BspT107I
BshTI	A/CCGGT	AgeI ^§	R0552	A/CCGGT	AgeI, AgeI-HF, AsiGI, CspAI, PinAI
		AgeI-HF	R3552	A/CCGGT
BshVI	AT/CGAT	BspDI	R0557	AT/CGAT	Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI
		ClaI	R0197	AT/CGAT
BsiEI	CGRY/CG	BsiEI	R0554	CGRY/CG	Bsh1285I, BstMCI
BsiHKAI	GWGCW/C	BsiHKAI	R0570	GWGCW/C	Alw21I, Bbv12I
BsiHKCI	C/YCGRG	AvaI	R0152	C/YCGRG	Ama87I, AvaI, BmeT110I, BsoBI, Eco88I
		BsoBI	R0586	C/YCGRG
BsiI x	CACGAG(-5/-1)	BssSI	R0680	CACGAG(-5/-1)	BauI, BssSI-v2, Bst2BI
		BssSI-v2	R0680	CACGAG(-5/-1)
BsiSI	C/CGG	HpaII	R0171	C/CGG	HapII, HpaII, MspI
		MspI	R0106	C/CGG
BsiWI	C/GTACG	BsiWI^§	R0553	C/GTACG	BsiWI-HF, Pfl23II, PspLI
		BsiWI-HF	R3553	C/GTACG
BsiWI-HF	C/GTACG	BsiWI^§	R0553	C/GTACG	BsiWI, Pfl23II, PspLI
		BsiWI-HF	R3553	C/GTACG
BsiYI x	CCNNNNN/NNGG	BslI	R0555	CCNNNNN/NNGG	AfiI, Bsc4I, BseLI, BslI
BslFI	GGGAC(10/14)	BsmFI	R0572	GGGAC(10/14)	BsmFI, FaqI
BslI	CCNNNNN/NNGG	BslI	R0555	CCNNNNN/NNGG	AfiI, Bsc4I, BseLI
BsmAI	GTCTC(1/5)	BcoDI	R0542	GTCTC(1/5)	Alw26I, BcoDI, BstMAI
		BsmAI	R0529	GTCTC(1/5)
BsmBI-v2	CGTCTC(1/5)	BsmBI-v2	R0739	CGTCTC(1/5)	Esp3I
		Esp3I	R0734	CGTCTC(1/5)
BsmFI	GGGAC(10/14)	BsmFI	R0572	GGGAC(10/14)	BslFI, FaqI
BsmI	GAATGC(1/-1)	BsmI	R0134	GAATGC(1/-1)	Mva1269I, PctI
BsnI	GG/CC	HaeIII	R0108	GG/CC	BshFI, BspANI, BsuRI, HaeIII
BsoBI	C/YCGRG	AvaI	R0152	C/YCGRG	Ama87I, AvaI, BmeT110I, BsiHKCI, Eco88I
		BsoBI	R0586	C/YCGRG
Bso31I	GGTCTC(1/5)	BsaI-HFv2	R3733	GGTCTC(1/5)	BsaI-HFv2, BspTNI, Eco31I
BspACI	CCGC(-3/-1)	AciI	R0551	CCGC(-3/-1)	AciI, SsiI
BspANI	GG/CC	HaeIII	R0108	GG/CC	BshFI, BsnI, BsuRI, HaeIII
BspCNI	CTCAG(9/7)	BspCNI	R0624	CTCAG(9/7)	BseMII^
BspDI	AT/CGAT	BspDI	R0557	AT/CGAT	Bsa29I, BseCI, BshVI, Bsu15I, BsuTUI, ClaI
		ClaI	R0197	AT/CGAT
BspEI	T/CCGGA	BspEI	R0540	T/CCGGA	AccIII, Aor13HI, BseAI, Bsp13I, Kpn2I, MroI
BspFNI	CG/CG	BstUI	R0518	CG/CG	AccII, Bsh1236I, BstFNI, BstUI, MvnI
BspGI x	CTGGAC
BspHI	T/CATGA	BspHI	R0517	T/CATGA	CciI, PagI
Bsp143I	/GATC	DpnII	R0543	/GATC	BssMI, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
		MboI	R0147	/GATC
		Sau3AI	R0169	/GATC
Bsp1407I	T/GTACA	BsrGI-HF	R3575	T/GTACA	BsrGI-HF, BstAUI
Bsp119I	TT/CGAA	BstBI	R0519	TT/CGAA	AsuII, Bpu14I, BspT104I, BstBI, NspV, SfuI
Bsp68I	TCG/CGA	NruI-HF	R3192	TCG/CGA	BtuMI, NruI-HF, RruI
Bsp1286I	GDGCH/C	Bsp1286I	R0120	GDGCH/C	MhlI, SduI
Bsp24I x	(8/13)GACNNNNNNTGG(12/7)
Bsp13I	T/CCGGA	BspEI	R0540	T/CCGGA	AccIII, Aor13HI, BseAI, BspEI, Kpn2I, MroI
Bsp19I	C/CATGG	NcoI ^§	R0193	C/CATGG	NcoI, NcoI-HF
		NcoI-HF	R3193	C/CATGG
Bsp120I	G/GGCCC	ApaI^	R0114	GGGCC/C	ApaI^, PspOMI
		PspOMI	R0653	G/GGCCC
Bsp1720I	GC/TNAGC	BlpI	R0585	GC/TNAGC	BlpI, Bpu1102I
Bsp460III x	CGCGCAG
Bsp3004IV x	CCGCAT
BspLI	GGN/NCC	NlaIV	R0126	GGN/NCC	BmiI, NlaIV, PspN4I
BspLU11I x	A/CATGT	PciI	R0655	A/CATGT	PciI, PscI
BspMAI	CTGCA/G	PstI^§	R0140	CTGCA/G	PstI, PstI-HF
		PstI-HF	R3140	CTGCA/G
BspMI	ACCTGC(4/8)	BfuAI	R0701	ACCTGC(4/8)	Acc36I, BfuAI, BveI
		BspMI	R0502	ACCTGC(4/8)
BspMII x	T/CCGGA	BspEI	R0540	T/CCGGA	AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I, MroI
BspNCI x	CCAGA
BspOI	GCTAG/C	BmtI-HF	R3658	GCTAG/C	AsuNHI^, BmtI-HF, NheI-HFv2^
		NheI-HF^	R3131	G/CTAGC
		NheI-HFv2^	R3131	G/CTAGC
BspPI	GGATC(4/5)	AlwI	R0513	GGATC(4/5)	AclWI, AlwI
BspQI	GCTCTTC(1/4)	BspQI	R0712	GCTCTTC(1/4)	LguI, PciSI, SapI
		SapI	R0569	GCTCTTC(1/4)
BspT107I	G/GYRCC	BanI	R0118	G/GYRCC	AccB1I, BanI, BshNI
BspT104I	TT/CGAA	BstBI	R0519	TT/CGAA	AsuII, Bpu14I, Bsp119I, BstBI, NspV, SfuI
BspTI	C/TTAAG	AflII	R0520	C/TTAAG	AflII, BfrI, BstAFI, MspCI, Vha464I
BspTNI	GGTCTC(1/5)	BsaI-HFv2	R3733	GGTCTC(1/5)	BsaI-HFv2, Bso31I, Eco31I
BsrBI	CCGCTC(-3/-3)	BsrBI	R0102	CCGCTC(-3/-3)	AccBSI, MbiI
BsrDI	GCAATG(2/0)	BsrDI	R0574	GCAATG(2/0)	Bse3DI, BseMI
BsrFI-v2	R/CCGGY	BsrFI-v2	R0682	R/CCGGY	Bse118I, BssAI, Cfr10I
BsrGI-HF	T/GTACA	BsrGI-HF	R3575	T/GTACA	Bsp1407I, BstAUI
BsrI	ACTGG(1/-1)	BsrI	R0527	ACTGG(1/-1)	Bse1I, BseNI
BssAI	R/CCGGY	BsrFI	R0682	R/CCGGY	Bse118I, BsrFI-v2, Cfr10I
		BsrFI-v2	R0682	R/CCGGY
BssECI	C/CNNGG	BsaJI	R0536	C/CNNGG	BsaJI, BseDI
BssHII	G/CGCGC	BssHII	R0199	G/CGCGC	BsePI, PauI, PteI
BssMI	/GATC	DpnII	R0543	/GATC	Bsp143I, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
		MboI	R0147	/GATC
		Sau3AI	R0169	/GATC
BssNAI	GTA/TAC	BstZ17I	R3594	GTA/TAC	Bst1107I, BstZ17I-HF
		BstZ17I-HF	R3594	GTA/TAC
BssNI	GR/CGYC	BsaHI	R0556	GR/CGYC	AcyI, BsaHI, BstACI, Hin1I, Hsp92I
BssSI-v2	CACGAG(-5/-1)	BssSI-v2	R0680	CACGAG(-5/-1)	BauI, Bst2BI
BssT1I	C/CWWGG	StyI-HF	R3500	C/CWWGG	Eco130I, EcoT14I, ErhI, StyI-HF
BstACI	GR/CGYC	BsaHI	R0556	GR/CGYC	AcyI, BsaHI, BssNI, Hin1I, Hsp92I
BstAFI	C/TTAAG	AflII	R0520	C/TTAAG	AflII, BfrI, BspTI, MspCI, Vha464I
BstAPI	GCANNNN/NTGC	BstAPI	R0654	GCANNNN/NTGC
BstAUI	T/GTACA	BsrGI-HF	R3575	T/GTACA	Bsp1407I, BsrGI-HF
BstBAI	YAC/GTR	BsaAI	R0531	YAC/GTR	BsaAI, Ppu21I
BstBI	TT/CGAA	BstBI	R0519	TT/CGAA	AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI
Bst2BI	CACGAG(-5/-1)	BssSI	R0680	CACGAG(-5/-1)	BauI, BssSI-v2
		BssSI-v2	R0680	CACGAG(-5/-1)
BstC8I	GCN/NGC	Cac8I	R0579	GCN/NGC	Cac8I
Bst4CI	ACN/GT	HpyCH4III	R0618	ACN/GT	HpyCH4III, TaaI
BstDEI	C/TNAG	DdeI	R0175	C/TNAG	DdeI, HpyF3I
BstDSI	C/CRYGG	BtgI	R0608	C/CRYGG	BtgI
BstEII-HF	G/GTNACC	BstEII-HF	R3162	G/GTNACC	BstPI, Eco91I, EcoO65I, PspEI
BstENI	CCTNN/NNNAGG	EcoNI	R0521	CCTNN/NNNAGG	EcoNI, XagI
BstF5I	GGATG(2/0)	BtsCI	R0647	GGATG(2/0)	BseGI, BtsCI, FokI^
		FokI^	R0109	GGATG(9/13)
BstFNI	CG/CG	BstUI	R0518	CG/CG	AccII, Bsh1236I, BspFNI, BstUI, MvnI
BstHHI	GCG/C	HhaI	R0139	GCG/C	AspLEI, CfoI, HhaI, Hin6I^, HinP1I^, HspAI^
		HinP1I^	R0124	G/CGC
BstH2I	RGCGC/Y	HaeII	R0107	RGCGC/Y	BfoI, HaeII
Bst6I	CTCTTC(1/4)	EarI	R0528	CTCTTC(1/4)	Eam1104I, EarI
Bst1107I	GTA/TAC	BstZ17I	R3594	GTA/TAC	BssNAI, BstZ17I-HF
		BstZ17I-HF	R3594	GTA/TAC
BstKTI	GAT/C	DpnII^	R0543	/GATC	Bsp143I^, BssMI^, BstMBI^, DpnII^, Kzo9I^, MboI^, NdeII^, Sau3AI^
		MboI^	R0147	/GATC
		Sau3AI^	R0169	/GATC
BstMAI	GTCTC(1/5)	BcoDI	R0542	GTCTC(1/5)	Alw26I, BcoDI, BsmAI
		BsmAI	R0529	GTCTC(1/5)
BstMBI	/GATC	DpnII	R0543	/GATC	Bsp143I, BssMI, BstKTI^, DpnII, Kzo9I, MboI, NdeII, Sau3AI
		MboI	R0147	/GATC
		Sau3AI	R0169	/GATC
BstMCI	CGRY/CG	BsiEI	R0554	CGRY/CG	Bsh1285I, BsiEI
BstMWI	GCNNNNN/NNGC	MwoI	R0573	GCNNNNN/NNGC	HpyF10VI, MwoI
BstNI	CC/WGG	BstNI	R0168	CC/WGG	AjnI^, BciT130I, BseBI, Bst2UI, EcoRII^, MvaI, Psp6I^, PspGI^
		PspGI^	R0611	/CCWGG
BstNSI	RCATG/Y	NspI	R0602	RCATG/Y	NspI, XceI
BstPAI	GACNN/NNGTC	PshAI	R0593	GACNN/NNGTC	BoxI, PshAI
BstPI	G/GTNACC	BstEII-HF	R3162	G/GTNACC	BstEII-HF, Eco91I, EcoO65I, PspEI
BstSCI	/CCNGG	ScrFI^	R0110	CC/NGG	Bme1390I^, BmrFI^, MspR9I^, ScrFI^, StyD4I
		StyD4I	R0638	/CCNGG
BstSFI	C/TRYAG	SfcI	R0561	C/TRYAG	BfmI, SfcI
BstSLI	GKGCM/C	BaeGI	R0708	GKGCM/C	BaeGI, BseSI
BstSNI	TAC/GTA	SnaBI	R0130	TAC/GTA	Eco105I, SnaBI
Bst2UI	CC/WGG	BstNI	R0168	CC/WGG	AjnI^, BciT130I, BseBI, BstNI, EcoRII^, MvaI, Psp6I^, PspGI^
		PspGI^	R0611	/CCWGG
BstUI	CG/CG	BstUI	R0518	CG/CG	AccII, Bsh1236I, BspFNI, BstFNI, MvnI
BstV2I	GAAGAC(2/6)	BbsI^§	R0539	GAAGAC(2/6)	BbsI, BbsI-HF, BpiI
		BbsI-HF	R3539	GAAGAC(2/6)
BstV1I	GCAGC(8/12)	BbvI	R0173	GCAGC(8/12)	BbvI, BseXI, Lsp1109I
BstXI	CCANNNNN/NTGG	BstXI	R0113	CCANNNNN/NTGG
BstX2I	R/GATCY	BstYI	R0523	R/GATCY	BstYI, MflI, PsuI
BstYI	R/GATCY	BstYI	R0523	R/GATCY	BstX2I, MflI, PsuI
BstZI	C/GGCCG	EagI-HF	R3505	C/GGCCG	BseX3I, EagI-HFv2, EclXI, Eco52I
		EagI-HFv2	R3505	C/GGCCG
BstZ17I-HF	GTA/TAC	BstZ17I-HF	R3594	GTA/TAC	BssNAI, Bst1107I
Bsu15I	AT/CGAT	BspDI	R0557	AT/CGAT	Bsa29I, BseCI, BshVI, BspDI, BsuTUI, ClaI
		ClaI	R0197	AT/CGAT
Bsu36I	CC/TNAGG	Bsu36I	R0524	CC/TNAGG	AxyI, Bse21I, Eco81I
BsuI	GTATCC(6/5)	BciVI	R0596	GTATCC(6/5)	BciVI, BfuI
BsuRI	GG/CC	HaeIII	R0108	GG/CC	BshFI, BsnI, BspANI, HaeIII
BsuTUI	AT/CGAT	BspDI	R0557	AT/CGAT	Bsa29I, BseCI, BshVI, BspDI, Bsu15I, ClaI
		ClaI	R0197	AT/CGAT
BtgI	C/CRYGG	BtgI	R0608	C/CRYGG	BstDSI
BtgZI	GCGATG(10/14)	BtgZI	R0703	GCGATG(10/14)
BtrI	CACGTC(-3/-3)	BmgBI	R0628	CACGTC(-3/-3)	AjiI, BmgBI
BtsCI	GGATG(2/0)	BtsCI	R0647	GGATG(2/0)	BseGI, BstF5I, FokI^
		FokI^	R0109	GGATG(9/13)
BtsIMutI	CAGTG(2/0)	BtsIMutI	R0664	CAGTG(2/0)
BtsI-v2	GCAGTG(2/0)	BtsI-v2	R0667	GCAGTG(2/0)
BtuMI	TCG/CGA	NruI-HF	R3192	TCG/CGA	Bsp68I, NruI-HF, RruI
Bve1B23I x	GACNNNNNTGG
BveI	ACCTGC(4/8)	BfuAI	R0701	ACCTGC(4/8)	Acc36I, BfuAI, BspMI
		BspMI	R0502	ACCTGC(4/8)
Cac8I	GCN/NGC	Cac8I	R0579	GCN/NGC	BstC8I
CaiI	CAGNNN/CTG	AlwNI	R0514	CAGNNN/CTG	AlwNI, PstNI
CalB3II x	GRTTRAG
Cal14237I x	GGTTAG
Cau10061II x	GTTAAT
CauII x	CC/SGG	NciI	R0196	CC/SGG	AsuC2I, BcnI, BpuMI, NciI
Cba16038I x	CCTNAYNC
Cba13II x	AGGAAT
Cbo67071IV x	GCRGAAG
CcaP7V x	CRAAAAR
CchII x	GGARGA(11/9)
Cch467III x	GNGAAAY
CchIII x	CCCAAG(20/18)
CciI	T/CATGA	BspHI	R0517	T/CATGA	BspHI, PagI
CciNI	GC/GGCCGC	NotI ^§	R0189	GC/GGCCGC	NotI, NotI-HF
		NotI-HF	R3189	GC/GGCCGC
Cco11437V x	CAYNNNNNRTAG
Cco14983V x	GGGTDA
Cco11366VI x	GAAGAA
Cco14983VI x	GCYGA
CcrNAIII x	CGACCAG
Cdi11397I x	GCGCAG
CdiI x	CATCG(-1/-1)
Cdi13750III x	CCGATCC
Cdi13746V x	RGAAAGR
CdpI x	GCGGAG(20/18)
Cdu23823II x	GTGAAG
Cfa8380I x	GRGGAY
CfoI	GCG/C	HhaI	R0139	GCG/C	AspLEI, BstHHI, HhaI, Hin6I^, HinP1I^, HspAI^
		HinP1I^	R0124	G/CGC
Cfr13I	G/GNCC	Sau96I	R0165	G/GNCC	AspS9I, BmgT120I, PspPI, Sau96I
Cfr9I	C/CCGGG	SmaI^	R0141	CCC/GGG	SmaI^, TspMI, XmaI
		TspMI	R0709	C/CCGGG
		XmaI	R0180	C/CCGGG
CfrI x	Y/GGCCR	EaeI	R0508	Y/GGCCR	AcoI, EaeI
Cfr10I	R/CCGGY	BsrFI	R0682	R/CCGGY	Bse118I, BsrFI-v2, BssAI
		BsrFI-v2	R0682	R/CCGGY
Cfr42I	CCGC/GG	SacII	R0157	CCGC/GG	KspI, SacII, Sfr303I, SgrBI
CfrMH13II x	AGCANCC
CfrMH16VI x	CTAAAG
Cfupf3II x	GARCAG
Cgl13032I x	GGCGCA
Cgl13032II x	ACGABGG
Cin11811I x	TGKMCA
CjeFIII x	GCAAGG
CjeFV x	GGRCA
CjeI x	(8/14)CCANNNNNNGT(15/9)
Cje54107III x	GKAAYC
CjeNII x	GAGNNNNNGT
CjeNIII x	GKAAYG(19/17)
CjeNV x	CCYGA
CjePI x	(7/13)CCANNNNNNNTC(14/8)
CjeP659IV x	CACNNNNNNNGAA
Cje265V x	GKAAGC
CjuI x	CAYNNNNNRTG
CjuII x	CAYNNNNNCTC
Cko11077IV x	TGACAG
ClaI	AT/CGAT	BspDI	R0557	AT/CGAT	Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI
		ClaI	R0197	AT/CGAT
Cla11845III x	GCGAA
Cly7489II x	AAAAGRG
Cpe13170II x	GTTGNAG
Cpe2837III x	GRNACAYT
Cpe10578V x	GANGAGY
CpoI	CG/GWCCG	RsrII	R0501	CG/GWCCG	CspI, RsrII, Rsr2I
Cre7908I x	GCGGGA
Csa9238II x	CAAANTC
CseI	GACGC(5/10)	HgaI	R0154	GACGC(5/10)	HgaI
CsiI	A/CCWGGT	SexAI	R0605	A/CCWGGT	MabI, SexAI
CspAI	A/CCGGT	AgeI ^§	R0552	A/CCGGT	AgeI, AgeI-HF, AsiGI, BshTI, PinAI
		AgeI-HF	R3552	A/CCGGT
CspBP25III x	CCANNNNNRTGA
CspCI	(11/13)CAANNNNNGTGG(12/10)	CspCI	R0645	(11/13)CAANNNNNGTGG(12/10)
Csp2014I x	GGAGGC
Csp6I	G/TAC	CviQI	R0639	G/TAC	AfaI^, CviQI, RsaI^, RsaNI
		RsaI^	R0167	GT/AC
CspI	CG/GWCCG	RsrII	R0501	CG/GWCCG	CpoI, RsrII, Rsr2I
CspL61I x	TYGAYCT
CspX1II x	ACCCCA
CstMI x	AAGGAG(20/18)
CviJI	RG/CY	CviKI-1	R0710	RG/CY	CviKI-1
CviKI-1	RG/CY	CviKI-1	R0710	RG/CY	CviJI
CviQI	G/TAC	CviQI	R0639	G/TAC	AfaI^, Csp6I, RsaI^, RsaNI
		RsaI^	R0167	GT/AC
CviRI x	TG/CA	HpyCH4V	R0620	TG/CA	HpyCH4V
Dde51507I x	CCWGG
DdeI	C/TNAG	DdeI	R0175	C/TNAG	BstDEI, HpyF3I
DinI	GGC/GCC	KasI^	R0544	G/GCGCC	EgeI, EheI, KasI^, Mly113I^^, NarI^^, PluTI^^^, SfoI, SspDI^
		NarI^^	R0191	GG/CGCC
		PluTI^^^	R0713	GGCGC/C
		SfoI	R0606	GGC/GCC
Dpi3069I x	GACAG
Dpi3084I x	CGRAG
Dpi3090II x	AAGRAG
DpnI	GA/TC	DpnI	R0176	GA/TC	MalI
DpnII	/GATC	DpnII	R0543	/GATC	Bsp143I, BssMI, BstKTI^, BstMBI, Kzo9I, MboI, NdeII, Sau3AI
		MboI	R0147	/GATC
		Sau3AI	R0169	/GATC
DraI	TTT/AAA	DraI	R0129	TTT/AAA
DraII x	RG/GNCCY	EcoO109I	R0503	RG/GNCCY	EcoO109I
DraIII-HF	CACNNN/GTG	DraIII-HF	R3510	CACNNN/GTG	AdeI
DraRI x	CAAGNAC(20/18)
DrdI	GACNNNN/NNGTC	DrdI	R0530	GACNNNN/NNGTC	AasI, DseDI
DrdII x	GAACCA
DrdIV x	TACGAC(20/18)
DrdV x	CATGNAC(10/8)
DrdVI x	GCAGCC
DrdVIII x	ARGAGC
DriI	GACNNN/NNGTC	AhdI	R0584	GACNNN/NNGTC	AhdI, BmeRI, Eam1105I
DsaI x	C/CRYGG	BtgI	R0608	C/CRYGG	BstDSI, BtgI
DseDI	GACNNNN/NNGTC	DrdI	R0530	GACNNNN/NNGTC	AasI, DrdI
DspS02II x	TGCCGAC
DvuIII x	CACNCAC
EaeI	Y/GGCCR	EaeI	R0508	Y/GGCCR	AcoI
EagI-HFv2	C/GGCCG	EagI-HFv2	R3505	C/GGCCG	BseX3I, BstZI, EclXI, Eco52I
Eam1104I	CTCTTC(1/4)	EarI	R0528	CTCTTC(1/4)	Bst6I, EarI
Eam1105I	GACNNN/NNGTC	AhdI	R0584	GACNNN/NNGTC	AhdI, BmeRI, DriI
EarI	CTCTTC(1/4)	EarI	R0528	CTCTTC(1/4)	Bst6I, Eam1104I
EciI	GGCGGA(11/9)	EciI	R0590	GGCGGA(11/9)
Ecl35734I x	GAAAYTC
Ecl234I x	CGGNAAG
Ecl136II	GAG/CTC	Eco53kI	R0116	GAG/CTC	EcoICRI, Eco53kI, Psp124BI^, SacI^, SacI-HF^, SstI^
		SacI^^§	R0156	GAGCT/C
		SacI-HF^	R3156	GAGCT/C
EclXI	C/GGCCG	EagI-HF	R3505	C/GGCCG	BseX3I, BstZI, EagI-HFv2, Eco52I
		EagI-HFv2	R3505	C/GGCCG
EcoBLMcrX x	RCSRC(-3/-2)
EcoE1140I x	ACCYAC
Eco72I	CAC/GTG	PmlI	R0532	CAC/GTG	AcvI, BbrPI, PmaCI, PmlI, PspCI
Eco32I	GAT/ATC	EcoRV ^§	R0195	GAT/ATC	EcoRV, EcoRV-HF
		EcoRV-HF	R3195	GAT/ATC
Eco57I	CTGAAG(16/14)	AcuI	R0641	CTGAAG(16/14)	AcuI
Eco52I	C/GGCCG	EagI-HF	R3505	C/GGCCG	BseX3I, BstZI, EagI-HFv2, EclXI
		EagI-HFv2	R3505	C/GGCCG
Eco147I	AGG/CCT	StuI	R0187	AGG/CCT	PceI, SseBI, StuI
Eco8164I x	GCCKAG
Eco4174I x	GCACAG
Eco88I	C/YCGRG	AvaI	R0152	C/YCGRG	Ama87I, AvaI, BmeT110I, BsiHKCI, BsoBI
		BsoBI	R0586	C/YCGRG
Eco130I	C/CWWGG	StyI-HF	R3500	C/CWWGG	BssT1I, EcoT14I, ErhI, StyI-HF
Eco105I	TAC/GTA	SnaBI	R0130	TAC/GTA	BstSNI, SnaBI
Eco91I	G/GTNACC	BstEII-HF	R3162	G/GTNACC	BstEII-HF, BstPI, EcoO65I, PspEI
Eco1836I x	CACANTT
Eco9020I x	CGAABTT
Eco9035I x	GGGANTT
Eco47I	G/GWCC	AvaII	R0153	G/GWCC	AvaII, Bme18I, SinI, VpaK11BI
Eco31I	GGTCTC(1/5)	BsaI-HFv2	R3733	GGTCTC(1/5)	BsaI-HFv2, Bso31I, BspTNI
		BsaI-HF-v2	R3733	GGTCTC(1/5)
Eco81I	CC/TNAGG	Bsu36I	R0524	CC/TNAGG	AxyI, Bse21I, Bsu36I
Eco24I	GRGCY/C	BanII	R0119	GRGCY/C	BanII, EcoT38I, FriOI
EcoICRI	GAG/CTC	Eco53kI	R0116	GAG/CTC	Ecl136II, Eco53kI, Psp124BI^, SacI^, SacI-HF^, SstI^
		SacI^^§	R0156	GAGCT/C
		SacI-HF^	R3156	GAGCT/C
Eco9009II x	GAAANTC
Eco9699II x	TAGARC
Eco43896II x	CRARCAG
Eco4465II x	GAAABCC
Eco47III	AGC/GCT	AfeI	R0652	AGC/GCT	AfeI, Aor51HI
Eco57MI x	CTGRAG(16/14)
EcoMVII x	CANCATC
EcoNI	CCTNN/NNNAGG	EcoNI	R0521	CCTNN/NNNAGG	BstENI, XagI
EcoNIH6II x	ATGAAG
EcoO65I	G/GTNACC	BstEII-HF	R3162	G/GTNACC	BstEII-HF, BstPI, Eco91I, PspEI
EcoO109I	RG/GNCCY	EcoO109I	R0503	RG/GNCCY
EcoRI	G/AATTC	EcoRI ^§	R0101	G/AATTC	EcoRI-HF
		EcoRI-HF	R3101	G/AATTC
EcoRI-HF	G/AATTC	EcoRI ^§	R0101	G/AATTC	EcoRI
		EcoRI-HF	R3101	G/AATTC
EcoRII	/CCWGG	BstNI^	R0168	CC/WGG	AjnI, BciT130I^, BseBI^, BstNI^, Bst2UI^, MvaI^, Psp6I, PspGI
		PspGI	R0611	/CCWGG
EcoRV	GAT/ATC	EcoRV ^§	R0195	GAT/ATC	Eco32I, EcoRV-HF
		EcoRV-HF	R3195	GAT/ATC
EcoRV-HF	GAT/ATC	EcoRV ^§	R0195	GAT/ATC	Eco32I, EcoRV
		EcoRV-HF	R3195	GAT/ATC
EcoT38I	GRGCY/C	BanII	R0119	GRGCY/C	BanII, Eco24I, FriOI
EcoT22I	ATGCA/T	NsiI^§	R0127	ATGCA/T	Mph1103I, NsiI, NsiI-HF, Zsp2I
		NsiI-HF	R3127	ATGCA/T
EcoT14I	C/CWWGG	StyI-HF	R3500	C/CWWGG	BssT1I, Eco130I, ErhI, StyI-HF
Eco53kI	GAG/CTC	Eco53kI	R0116	GAG/CTC	Ecl136II, EcoICRI, Psp124BI^, SacI^, SacI-HF^, SstI^
		SacI^^§	R0156	GAGCT/C
		SacI-HF^	R3156	GAGCT/C
EgeI	GGC/GCC	KasI^	R0544	G/GCGCC	DinI, EheI, KasI^, Mly113I^^, NarI^^, PluTI^^^, SfoI, SspDI^
		NarI^^	R0191	GG/CGCC
		PluTI^^^	R0713	GGCGC/C
		SfoI	R0606	GGC/GCC
EheI	GGC/GCC	KasI^	R0544	G/GCGCC	DinI, EgeI, KasI^, Mly113I^^, NarI^^, PluTI^^^, SfoI, SspDI^
		NarI^^	R0191	GG/CGCC
		PluTI^^^	R0713	GGCGC/C
		SfoI	R0606	GGC/GCC
Ehi46392I x	CCCNNAG
Eli8509II x	CCGGAG
ErhG4T10I x	CGANNNNNNTC
ErhI	C/CWWGG	StyI-HF	R3500	C/CWWGG	BssT1I, Eco130I, EcoT14I, StyI-HF
EsaSSI x	GACCAC
Esp3007I x	CAGAAG
Esp3I	CGTCTC(1/5)	BsmBI-v2	R0739	CGTCTC(1/5)	BsmBI-v2
		Esp3I	R0734	CGTCTC(1/5)
EspI x	GC/TNAGC	BlpI	R0585	GC/TNAGC	BlpI, Bpu1102I, Bsp1720I
FaeI	CATG/	FatI^	R0650	/CATG	FatI^, Hin1II, Hsp92II, NlaIII
		NlaIII	R0125	CATG/
FaiI	YA/TR
FalI	(8/13)AAGNNNNNCTT(13/8)
FaqI	GGGAC(10/14)	BsmFI	R0572	GGGAC(10/14)	BslFI, BsmFI
FatI	/CATG	FatI	R0650	/CATG	FaeI^, Hin1II^, Hsp92II^, NlaIII^
		NlaIII^	R0125	CATG/
FauI	CCCGC(4/6)	FauI	R0651	CCCGC(4/6)
FauNDI	CA/TATG	NdeI	R0111	CA/TATG	NdeI
FbaI	T/GATCA	BclI^§	R0160	T/GATCA	BclI, BclI-HF, Ksp22I
		BclI-HF	R3160	T/GATCA
Fba202Z8II x	AGAAGG
FblI	GT/MKAC	AccI	R0161	GT/MKAC	AccI, XmiI
Fco1691IV x	GCVGAG
FinI x	GGGAC	BsmFI	R0572	GGGAC(10/14)	BslFI, BsmFI, FaqI
Fna13121I x	TTGAYC
FnuDII x	CG/CG	BstUI	R0518	CG/CG	AccII, Bsh1236I, BspFNI, BstFNI, BstUI, MvnI
Fnu4HI	GC/NGC	Fnu4HI	R0178	GC/NGC	Fsp4HI, SatI
Fnu11326II x	GAGNNNNRTAY
Fnu11326IV x	CTTAATT
FokI	GGATG(9/13)	BtsCI^	R0647	GGATG(2/0)	BseGI^, BstF5I^, BtsCI^
		FokI	R0109	GGATG(9/13)
FriOI	GRGCY/C	BanII	R0119	GRGCY/C	BanII, Eco24I, EcoT38I
FseI	GGCCGG/CC	FseI	R0588	GGCCGG/CC	RigI
FspAI	RTGC/GCAY
FspBI	C/TAG	BfaI	R0568	C/TAG	BfaI, MaeI, SspMI, XspI
FspEI x	CC(12/16)
Fsp4HI	GC/NGC	Fnu4HI	R0178	GC/NGC	Fnu4HI, SatI
FspI	TGC/GCA	FspI	R0135	TGC/GCA	Acc16I, NsbI
FspPK15I x	GARGAAG
FtnUV x	GAAACA
GauT27I x	CGCGCAGG
Gba708II x	ATGCAC
GdiII x	CGGCCR(-5/-1)
GlaI	GC/GC
GluI	GC/NGC				BisI, BlsI^, PkrI^
Gru56503II x	CARABGC
GsaI	CCCAGC(-1/-5)	BseYI^	R0635	CCCAGC(-5/-1)	BseYI^, PspFI^
GsuI	CTGGAG(16/14)	BpmI	R0565	CTGGAG(16/14)	BpmI
GsuPI x	GTACAG
HaeI x	WGG/CCW
HaeII	RGCGC/Y	HaeII	R0107	RGCGC/Y	BfoI, BstH2I
HaeIII	GG/CC	HaeIII	R0108	GG/CC	BshFI, BsnI, BspANI, BsuRI
HaeIV x	(7/13)GAYNNNNNRTC(14/9)
HapII	C/CGG	HpaII	R0171	C/CGG	BsiSI, HpaII, MspI
		MspI	R0106	C/CGG
HbaII x	GCCCAG
Hca13221V x	CACNNNNNRTAY
HdeNY26I x	CGANNNNNNTCC
HdeZA17I x	GCANNNNNNTCC
HgaI	GACGC(5/10)	HgaI	R0154	GACGC(5/10)	CseI
HgiAI x	GWGCW/C	BsiHKAI	R0570	GWGCW/C	Alw21I, Bbv12I, BsiHKAI
HgiCI x	G/GYRCC	BanI	R0118	G/GYRCC	AccB1I, BanI, BshNI, BspT107I
HgiEII x	ACCNNNNNNGGT
HgiJII x	GRGCY/C	BanII	R0119	GRGCY/C	BanII, Eco24I, EcoT38I, FriOI
HhaI	GCG/C	HhaI	R0139	GCG/C	AspLEI, BstHHI, CfoI, Hin6I^, HinP1I^, HspAI^
		HinP1I^	R0124	G/CGC
Hin1I	GR/CGYC	BsaHI	R0556	GR/CGYC	AcyI, BsaHI, BssNI, BstACI, Hsp92I
Hin4I x	(8/13)GAYNNNNNVTC(13/8)
Hin6I	G/CGC	HhaI^	R0139	GCG/C	AspLEI^, BstHHI^, CfoI^, HhaI^, HinP1I, HspAI
		HinP1I	R0124	G/CGC
Hin4II x	CCTTC(6/5)	HpyAV	R0621	CCTTC(6/5)	HpyAV
Hin1II	CATG/	FatI^	R0650	/CATG	FaeI, FatI^, Hsp92II, NlaIII
		NlaIII	R0125	CATG/
HinP1I	G/CGC	HhaI^	R0139	GCG/C	AspLEI^, BstHHI^, CfoI^, HhaI^, Hin6I, HspAI
		HinP1I	R0124	G/CGC
HincII	GTY/RAC	HincII	R0103	GTY/RAC	HindII
HindII	GTY/RAC	HincII	R0103	GTY/RAC	HincII
HindIII	A/AGCTT	HindIII^§	R0104	A/AGCTT	HindIII-HF
		HindIII-HF	R3104	A/AGCTT
HindIII-HF	A/AGCTT	HindIII^§	R0104	A/AGCTT	HindIII
		HindIII-HF	R3104	A/AGCTT
HinfI	G/ANTC	HinfI	R0155	G/ANTC
HpaI	GTT/AAC	HpaI	R0105	GTT/AAC	KspAI
HpaII	C/CGG	HpaII	R0171	C/CGG	BsiSI, HapII, MspI
		MspI	R0106	C/CGG
HphI	GGTGA(8/7)	HphI	R0158	GGTGA(8/7)	AsuHPI
HpyAS001VI x	CYANNNNNNTTC
HpyAV	CCTTC(6/5)	HpyAV	R0621	CCTTC(6/5)
HpyAXIV x	GCGTA
HpyAXVIII x	GGANNAG
HpyAXVI-mut1 x	CRTTAA
HpyAXVI-mut2 x	CRTCNA
HpyCH4III	ACN/GT	HpyCH4III	R0618	ACN/GT	Bst4CI, TaaI
HpyCH4IV	A/CGT	HpyCH4IV	R0619	A/CGT	HpySE526I, MaeII, TaiI^
HpyCH4V	TG/CA	HpyCH4V	R0620	TG/CA
HpyF3I	C/TNAG	DdeI	R0175	C/TNAG	BstDEI, DdeI
HpyF10VI	GCNNNNN/NNGC	MwoI	R0573	GCNNNNN/NNGC	BstMWI, MwoI
HpyG272XV x	GAAAAG
Hpy188I	TCN/GA	Hpy188I	R0617	TCN/GA
Hpy99I	CGWCG/	Hpy99I	R0615	CGWCG/
Hpy8I	GTN/NAC	Hpy166II	R0616	GTN/NAC	Hpy166II
Hpy166II	GTN/NAC	Hpy166II	R0616	GTN/NAC	Hpy8I
Hpy188III	TC/NNGA	Hpy188III	R0622	TC/NNGA
Hpy178III x	TC/NNGA	Hpy188III	R0622	TC/NNGA	Hpy188III
HpyLIM6XII x	CYANNNNNNTCC
HpyLIM9XVI x	GAAAAG
HpyPU007XIX x	CYANNNNNNTGY
HpySE526I	A/CGT	HpyCH4IV	R0619	A/CGT	HpyCH4IV, MaeII, TaiI^
HpyUM037X x	GTGGNAG, TNGGNAG
HpyUM032XIII x	CYANNNNNNNTRG
HpyUM032XIII-mut1 x	CYANNNNNNNTTC
HpyUM032XIV x	GAAAG
Hpy300XI x	CCTYNA
Hpy99XIII x	GCCTA
Hpy99XIV x	GGWTAA
Hpy99XIV-mut1 x	GGWCNA
Hpy99XXII x	CYANNNNNNTGA
Hso63373III x	CGANNNNNRTAY
Hso63250IV x	AACNNNNNGTT
HspAI	G/CGC	HhaI^	R0139	GCG/C	AspLEI^, BstHHI^, CfoI^, HhaI^, Hin6I, HinP1I
		HinP1I	R0124	G/CGC
Hsp92I	GR/CGYC	BsaHI	R0556	GR/CGYC	AcyI, BsaHI, BssNI, BstACI, Hin1I
Hsp92II	CATG/	FatI^	R0650	/CATG	FaeI, FatI^, Hin1II, NlaIII
		NlaIII	R0125	CATG/
HspMHR1II x	GAGCAGC
Jma19592I x	GTATNAC
Jma19592II x	GRGCRAC
Jsp2502II x	GRNGAAT
KasI	G/GCGCC	KasI	R0544	G/GCGCC	DinI^, EgeI^, EheI^, Mly113I^^, NarI^^, PluTI^^^, SfoI^, SspDI
		NarI^^	R0191	GG/CGCC
		PluTI^^^	R0713	GGCGC/C
		SfoI^	R0606	GGC/GCC
Kas9737III x	CCCRAG
KflI	GG/GWCCC
Kor51II x	RTCGAG
Kpn9178I x	GNGCGAG
Kpn2I	T/CCGGA	BspEI	R0540	T/CCGGA	AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI
KpnI	GGTAC/C	Acc65I^	R0599	G/GTACC	Acc65I^, Asp718I^, KpnI-HF
		KpnI ^§	R0142	GGTAC/C
		KpnI-HF	R3142	GGTAC/C
Kpn327I x	GACATC
KpnI-HF	GGTAC/C	Acc65I^	R0599	G/GTACC	Acc65I^, Asp718I^, KpnI
		KpnI ^§	R0142	GGTAC/C
		KpnI-HF	R3142	GGTAC/C
Kpn9644II x	GRACRAC
KpnNH25III x	CTRGAG
KpnNIH50I x	GCYAAG
KpnNIH30III x	GTTCNAC
Kpn156V x	CRTGATT
KroI	G/CCGGC
Kro7512II x	ARCAGKC
KroNI	GCC/GGC	NaeI	R0190	GCC/GGC	MroNI^, NaeI, NgoMIV^, PdiI
		NgoMIV^	R0564	G/CCGGC
KspAI	GTT/AAC	HpaI	R0105	GTT/AAC	HpaI
KspI	CCGC/GG	SacII	R0157	CCGC/GG	Cfr42I, SacII, Sfr303I, SgrBI
Ksp22I	T/GATCA	BclI^§	R0160	T/GATCA	BclI, BclI-HF, FbaI
		BclI-HF	R3160	T/GATCA
Ksp632I x	CTCTTC(1/4)	EarI	R0528	CTCTTC(1/4)	Bst6I, Eam1104I, EarI
Kzo9I	/GATC	DpnII	R0543	/GATC	Bsp143I, BssMI, BstKTI^, BstMBI, DpnII, MboI, NdeII, Sau3AI
		MboI	R0147	/GATC
		Sau3AI	R0169	/GATC
Lba2029III x	CYAAANG
Lbr124II x	CATCNAC
Lcr047I x	CTCCA
Lcr310I x	CAAAGC
LcrJM3I x	GRTAAAT
LcrJM3II x	GMAGG
LcrJM1V x	AGCYAC
Lcr147VIII x	GGYAAG
Lde4408II x	ACAAAG
LguI	GCTCTTC(1/4)	BspQI	R0712	GCTCTTC(1/4)	BspQI, PciSI, SapI
		SapI	R0569	GCTCTTC(1/4)
LlaG50I x	CCGTKA
Lme32I x	CTYCAA
LmnI	GCTCC(1/-1)
Lmo370I x	AGCGCCG
Lmo911II x	TAGRAG
Lpl1004II x	AGGRAG
Lpn12272I x	GCNCAAC
Lpn11417II x	ACGAAT
LpnPI x	CCG(11/15), CGG, GCGC
Lra68I x	GTTCNAG
LsaDS4I x	TGGAAT
Lsp1109I	GCAGC(8/12)	BbvI	R0173	GCAGC(8/12)	BbvI, BseXI, BstV1I
Lsp48III x	AGCACC
Lsp6406VI x	CRAGCAC
LweI	GCATC(5/9)	SfaNI	R0172	GCATC(5/9)	BmsI, SfaNI
MabI	A/CCWGGT	SexAI	R0605	A/CCWGGT	CsiI, SexAI
MaeI	C/TAG	BfaI	R0568	C/TAG	BfaI, FspBI, SspMI, XspI
MaeII	A/CGT	HpyCH4IV	R0619	A/CGT	HpyCH4IV, HpySE526I, TaiI^
MaeIII	/GTNAC
MalI	GA/TC	DpnI	R0176	GA/TC	DpnI
MaqI x	CRTTGAC(21/19)
MauBI	CG/CGCGCG
Mba11I x	AGGCGA
MbiI	CCGCTC(-3/-3)	BsrBI	R0102	CCGCTC(-3/-3)	AccBSI, BsrBI
MboI	/GATC	DpnII	R0543	/GATC	Bsp143I, BssMI, BstKTI^, BstMBI, DpnII, Kzo9I, NdeII, Sau3AI
		MboI	R0147	/GATC
		Sau3AI	R0169	/GATC
MboII	GAAGA(8/7)	MboII	R0148	GAAGA(8/7)
Mch10819I x	CYCAGCG
Mch946II x	WCGATCT
McrI x	CGRY/CG	BsiEI	R0554	CGRY/CG	Bsh1285I, BsiEI, BstMCI
MfeI	C/AATTG	MfeI ^§	R0589	C/AATTG	MfeI-HF, MunI
		MfeI-HF	R3589	C/AATTG
MfeI-HF	C/AATTG	MfeI ^§	R0589	C/AATTG	MfeI, MunI
		MfeI-HF	R3589	C/AATTG
MflI	R/GATCY	BstYI	R0523	R/GATCY	BstX2I, BstYI, PsuI
MhlI	GDGCH/C	Bsp1286I	R0120	GDGCH/C	Bsp1286I, SduI
MjaIV x	GTNNAC	Hpy166II	R0616	GTN/NAC	Hpy8I, Hpy166II
MkaDII x	GAGAYGT
Mla10359I x	CGANNNNNNTCA
MlsI	TGG/CCA	MscI	R0534	TGG/CCA	BalI, MluNI, Mox20I, MscI, Msp20I
MluCI	/AATT	MluCI	R0538	/AATT	Sse9I, TasI
MluI-HF	A/CGCGT	MluI-HF	R3198	A/CGCGT
Mlu211III x	AGCCCA
MluNI	TGG/CCA	MscI	R0534	TGG/CCA	BalI, MlsI, Mox20I, MscI, Msp20I
MlyI	GAGTC(5/5)	MlyI	R0610	GAGTC(5/5)	PleI^, PpsI^, SchI
		PleI^	R0515	GAGTC(4/5)
Mly113I	GG/CGCC	KasI^	R0544	G/GCGCC	DinI^^, EgeI^^, EheI^^, KasI^, NarI, PluTI^^^, SfoI^^, SspDI^
		NarI	R0191	GG/CGCC
		PluTI^^^	R0713	GGCGC/C
		SfoI^^	R0606	GGC/GCC
MmeI	TCCRAC(20/18)	MmeI	R0637	TCCRAC(20/18)
MnlI	CCTC(7/6)	MnlI	R0163	CCTC(7/6)
Mox20I	TGG/CCA	MscI	R0534	TGG/CCA	BalI, MlsI, MluNI, MscI, Msp20I
Mph1103I	ATGCA/T	NsiI^§	R0127	ATGCA/T	EcoT22I, NsiI, NsiI-HF, Zsp2I
		NsiI-HF	R3127	ATGCA/T
MreI	CG/CCGGCG
MroI	T/CCGGA	BspEI	R0540	T/CCGGA	AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I
MroNI	G/CCGGC	NaeI^	R0190	GCC/GGC	KroNI^, NaeI^, NgoMIV, PdiI^
		NgoMIV	R0564	G/CCGGC
MroXI	GAANN/NNTTC	XmnI	R0194	GAANN/NNTTC	Asp700I, PdmI, XmnI
MscI	TGG/CCA	MscI	R0534	TGG/CCA	BalI, MlsI, MluNI, Mox20I, Msp20I
MseI	T/TAA	MseI	R0525	T/TAA	SaqAI, Tru1I, Tru9I
MslI	CAYNN/NNRTG	MslI	R0571	CAYNN/NNRTG	RseI, SmiMI
MspA1I	CMG/CKG	MspA1I	R0577	CMG/CKG
MspCI	C/TTAAG	AflII	R0520	C/TTAAG	AflII, BfrI, BspTI, BstAFI, Vha464I
MspF392I x	CCCAATV
MspI	C/CGG	HpaII	R0171	C/CGG	BsiSI, HapII, HpaII
		MspI	R0106	C/CGG
Msp20I	TGG/CCA	MscI	R0534	TGG/CCA	BalI, MlsI, MluNI, Mox20I, MscI
MspI7II x	ACGRAG
MspI7IV x	GCMGAAG
MspJI	CNNR(9/13)	MspJI	R0661	CNNR(9/13)
MspR9I	CC/NGG	ScrFI	R0110	CC/NGG	Bme1390I, BmrFI, BstSCI^, ScrFI, StyD4I^
		StyD4I^	R0638	/CCNGG
MspSC27II x	CCGCGAC
MssI	GTTT/AAAC	PmeI	R0560	GTTT/AAAC	PmeI
MstI x	TGC/GCA	FspI	R0135	TGC/GCA	Acc16I, FspI, NsbI
MteI	GCGC/NGCGC
MtuHN878II x	CACGCAG
MunI	C/AATTG	MfeI ^§	R0589	C/AATTG	MfeI, MfeI-HF
		MfeI-HF	R3589	C/AATTG
Mva1269I	GAATGC(1/-1)	BsmI	R0134	GAATGC(1/-1)	BsmI, PctI
MvaI	CC/WGG	BstNI	R0168	CC/WGG	AjnI^, BciT130I, BseBI, BstNI, Bst2UI, EcoRII^, Psp6I^, PspGI^
		PspGI^	R0611	/CCWGG
MvnI	CG/CG	BstUI	R0518	CG/CG	AccII, Bsh1236I, BspFNI, BstFNI, BstUI
MwoI	GCNNNNN/NNGC	MwoI	R0573	GCNNNNN/NNGC	BstMWI, HpyF10VI
NaeI	GCC/GGC	NaeI	R0190	GCC/GGC	KroNI, MroNI^, NgoMIV^, PdiI
		NgoMIV^	R0564	G/CCGGC
Nal45188II x	ACCAGC
Nan12227I x	CCANNNNNNTCY
NarI	GG/CGCC	KasI^	R0544	G/GCGCC	DinI^^, EgeI^^, EheI^^, KasI^, Mly113I, PluTI^^^, SfoI^^, SspDI^
		NarI	R0191	GG/CGCC
		PluTI^^^	R0713	GGCGC/C
		SfoI^^	R0606	GGC/GCC
Nbr128II x	ACCGAC
NciI	CC/SGG	NciI	R0196	CC/SGG	AsuC2I, BcnI, BpuMI
NcoI	C/CATGG	NcoI ^§	R0193	C/CATGG	Bsp19I, NcoI-HF
		NcoI-HF	R3193	C/CATGG
NcoI-HF	C/CATGG	NcoI ^§	R0193	C/CATGG	Bsp19I, NcoI
		NcoI-HF	R3193	C/CATGG
NdeI	CA/TATG	NdeI	R0111	CA/TATG	FauNDI
NdeII	/GATC	DpnII	R0543	/GATC	Bsp143I, BssMI, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, Sau3AI
		MboI	R0147	/GATC
		Sau3AI	R0169	/GATC
NgoAVII x	GCCGC(7/7)
NgoAVIII x	(12/14)GACNNNNNTGA(13/11)
NgoMIV	G/CCGGC	NaeI^	R0190	GCC/GGC	KroNI^, MroNI, NaeI^, PdiI^
		NgoMIV	R0564	G/CCGGC
NhaXI x	CAAGRAG
NheI-HFv2	G/CTAGC	BmtI-HF^	R3658	GCTAG/C	AsuNHI, BmtI-HF^, BspOI^
		NheI-HFv2	R3131	G/CTAGC
NhoI x	GCWGC
NlaCI x	CATCAC(19/17)
NlaIII	CATG/	FatI^	R0650	/CATG	FaeI, FatI^, Hin1II, Hsp92II
		NlaIII	R0125	CATG/
NlaIV	GGN/NCC	NlaIV	R0126	GGN/NCC	BmiI, BspLI, PspN4I
NmeA6CIII x	GCCGAC(21/19)
NmeAIII	GCCGAG(21/19)	NmeAIII	R0711	GCCGAG(21/19)
NmuCI	/GTSAC	Tsp45I	R0583	/GTSAC	TseFI, Tsp45I
NotI	GC/GGCCGC	NotI ^§	R0189	GC/GGCCGC	CciNI, NotI-HF
		NotI-HF	R3189	GC/GGCCGC
NotI-HF	GC/GGCCGC	NotI ^§	R0189	GC/GGCCGC	CciNI, NotI
		NotI-HF	R3189	GC/GGCCGC
NpeUS61II x	GATCGAC
NruI-HF	TCG/CGA	NruI-HF	R3192	TCG/CGA	Bsp68I, BtuMI, RruI
NsbI	TGC/GCA	FspI	R0135	TGC/GCA	Acc16I, FspI
NsiI	ATGCA/T	NsiI^§	R0127	ATGCA/T	EcoT22I, Mph1103I, NsiI-HF, Zsp2I
		NsiI-HF	R3127	ATGCA/T
NsiI-HF	ATGCA/T	NsiI^§	R0127	ATGCA/T	EcoT22I, Mph1103I, NsiI, Zsp2I
		NsiI-HF	R3127	ATGCA/T
NspBII x	CMG/CKG	MspA1I	R0577	CMG/CKG	MspA1I
NspES21II x	CRTTCAG
NspI	RCATG/Y	NspI	R0602	RCATG/Y	BstNSI, XceI
NspV	TT/CGAA	BstBI	R0519	TT/CGAA	AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, SfuI
ObaBS10I x	ACGAG
OgrI x	CAACNAC
OliI	CACNN/NNGTG	AleI-v2	R0685	CACNN/NNGTG	AleI-v2
OspHL35III x	YAGGAG
PacI	TTAAT/TAA	PacI	R0547	TTAAT/TAA
Pac19842II x	CCTTGA
PacIII x	GTAATC
PaeI	GCATG/C	SphI^§	R0182	GCATG/C	SphI, SphI-HF
		SphI-HF	R3182	GCATG/C
Pae8506I x	CATCGAR
Pae10662III x	TGACGAG
PaePA99III x	AAGAYC
PaeR7I	C/TCGAG	PaeR7I	R0177	C/TCGAG	Sfr274I, SlaI, XhoI
		XhoI	R0146	C/TCGAG
PagI	T/CATGA	BspHI	R0517	T/CATGA	BspHI, CciI
PalAI	GG/CGCGCC	AscI	R0558	GG/CGCGCC	AscI, SgsI
Pal408I x	CCRTGAG
PaqCI	CACCTGC(4/8)	PaqCI	R0745	CACCTGC(4/8)	AarI
PasI	CC/CWGGG
PauI	G/CGCGC	BssHII	R0199	G/CGCGC	BsePI, BssHII, PteI
Pba2294I x	GTAAG
Pbu13063II x	GTATYC
PcaII x	GACGAG
PceI	AGG/CCT	StuI	R0187	AGG/CCT	Eco147I, SseBI, StuI
PciI	A/CATGT	PciI	R0655	A/CATGT	PscI
PciSI	GCTCTTC(1/4)	BspQI	R0712	GCTCTTC(1/4)	BspQI, LguI, SapI
		SapI	R0569	GCTCTTC(1/4)
Pcr308II x	CCAAAG
PcsI	WCGNNNN/NNNCGW
PctI	GAATGC(1/-1)	BsmI	R0134	GAATGC(1/-1)	BsmI, Mva1269I
PdiI	GCC/GGC	NaeI	R0190	GCC/GGC	KroNI, MroNI^, NaeI, NgoMIV^
		NgoMIV^	R0564	G/CCGGC
Pdi8503III x	CCGGNAG
PdmI	GAANN/NNTTC	XmnI	R0194	GAANN/NNTTC	Asp700I, MroXI, XmnI
Pdu1735I x	CACCAC
PenI x	GCAGT
PfeI	G/AWTC	TfiI	R0546	G/AWTC	TfiI
PflFI	GACN/NNGTC	PflFI	R0595	GACN/NNGTC	PsyI, Tth111I
		Tth111I	R0185	GACN/NNGTC
Pfl8569I x	GCN/NGC
Pfl1108I x	TCGTAG
Pfl23II	C/GTACG	BsiWI^§	R0553	C/GTACG	BsiWI, BsiWI-HF, PspLI
		BsiWI-HF	R3553	C/GTACG
Pfl10783II x	GCGTCAG
Pfl3756II x	CCCTNAG
PflMI	CCANNNN/NTGG	PflMI	R0509	CCANNNN/NTGG	AccB7I, Van91I
PflPt14I x	RGCCCAC
PfoI	T/CCNGGA
PfrJS15III x	CTTCNAC
PfrJS12IV x	TANAAG
PfrJS12V x	GGCGGAG
PgaP73III x	TTCGAG
PinAI	A/CCGGT	AgeI ^§	R0552	A/CCGGT	AgeI, AgeI-HF, AsiGI, BshTI, CspAI
		AgeI-HF	R3552	A/CCGGT
Pin17FIII x	GGYGAB
PinP23II x	CTRKCAG
PinP59III x	GAAGNAG
PkrI	GCN/GC				BisI^, BlsI, GluI^
Ple19I	CGAT/CG	PvuI-HF	R3150	CGAT/CG	PvuI-HF
PleI	GAGTC(4/5)	MlyI^	R0610	GAGTC(5/5)	MlyI^, PpsI, SchI^
		PleI	R0515	GAGTC(4/5)
PliMI x	CGCCGAC
PluTI	GGCGC/C	KasI^	R0544	G/GCGCC	DinI^^, EgeI^^, EheI^^, KasI^, Mly113I^^^, NarI^^^, SfoI^^, SspDI^
		NarI^^^	R0191	GG/CGCC
		PluTI	R0713	GGCGC/C
		SfoI^^	R0606	GGC/GCC
PmaCI	CAC/GTG	PmlI	R0532	CAC/GTG	AcvI, BbrPI, Eco72I, PmlI, PspCI
Pme10899I x	GACAGG
PmeI	GTTT/AAAC	PmeI	R0560	GTTT/AAAC	MssI
PmlI	CAC/GTG	PmlI	R0532	CAC/GTG	AcvI, BbrPI, Eco72I, PmaCI, PspCI
PpiI x	(7/12)GAACNNNNNCTC(13/8)
PpiP13II x	CGCRGAC
PpsI	GAGTC(4/5)	MlyI^	R0610	GAGTC(5/5)	MlyI^, PleI, SchI^
		PleI	R0515	GAGTC(4/5)
Ppu21I	YAC/GTR	BsaAI	R0531	YAC/GTR	BsaAI, BstBAI
PpuMI	RG/GWCCY	PpuMI	R0506	RG/GWCCY	Psp5II, PspPPI
Pru8113I x	CAGANGC
PscI	A/CATGT	PciI	R0655	A/CATGT	PciI
Pse18267I x	RCCGAAG
PshAI	GACNN/NNGTC	PshAI	R0593	GACNN/NNGTC	BoxI, BstPAI
PshBI	AT/TAAT	AseI	R0526	AT/TAAT	AseI, VspI
PsiI-v2	TTA/TAA	PsiI-v2	R0744	TTA/TAA	AanI
PspAT13III x	CCGANAG
Psp124BI	GAGCT/C	Eco53kI^	R0116	GAG/CTC	Ecl136II^, EcoICRI^, Eco53kI^, SacI, SacI-HF, SstI
		SacI^§	R0156	GAGCT/C
		SacI-HF	R3156	GAGCT/C
PspCI	CAC/GTG	PmlI	R0532	CAC/GTG	AcvI, BbrPI, Eco72I, PmaCI, PmlI
PspD7DII x	CCGCGAG
PspEI	G/GTNACC	BstEII-HF	R3162	G/GTNACC	BstEII-HF, BstPI, Eco91I, EcoO65I
PspFI	CCCAGC(-5/-1)	BseYI	R0635	CCCAGC(-5/-1)	BseYI, GsaI^
PspGI	/CCWGG	BstNI^	R0168	CC/WGG	AjnI, BciT130I^, BseBI^, BstNI^, Bst2UI^, EcoRII, MvaI^, Psp6I
		PspGI	R0611	/CCWGG
Psp1406I	AA/CGTT	AclI	R0598	AA/CGTT	AclI
Psp6I	/CCWGG	BstNI^	R0168	CC/WGG	AjnI, BciT130I^, BseBI^, BstNI^, Bst2UI^, EcoRII, MvaI^, PspGI
		PspGI	R0611	/CCWGG
Psp1314I x	GCTGCTG
Psp5II	RG/GWCCY	PpuMI	R0506	RG/GWCCY	PpuMI, PspPPI
Psp0357II x	GCGAAG
PspLI	C/GTACG	BsiWI^§	R0553	C/GTACG	BsiWI, BsiWI-HF, Pfl23II
		BsiWI-HF	R3553	C/GTACG
PspMR102II x	CAAGAAC
PspN4I	GGN/NCC	NlaIV	R0126	GGN/NCC	BmiI, BspLI, NlaIV
PspOMI	G/GGCCC	ApaI^	R0114	GGGCC/C	ApaI^, Bsp120I
		PspOMI	R0653	G/GGCCC
PspOMII x	CGCCCAR(20/18)
PspPI	G/GNCC	Sau96I	R0165	G/GNCC	AspS9I, BmgT120I, Cfr13I, Sau96I
PspPPI	RG/GWCCY	PpuMI	R0506	RG/GWCCY	PpuMI, Psp5II
PspPRI x	CCYCAG(15/13)
PspR84I x	TACYCAC
PspXI	VC/TCGAGB	PspXI	R0656	VC/TCGAGB
PsrI	(7/12)GAACNNNNNNTAC(12/7)
Pst145I x	CTAMRAG
Pst14472I x	CNYACAC
PstI	CTGCA/G	PstI^§	R0140	CTGCA/G	BspMAI, PstI-HF
		PstI-HF	R3140	CTGCA/G
Pst273I x	GATCGAG
PstI-HF	CTGCA/G	PstI^§	R0140	CTGCA/G	BspMAI, PstI
		PstI-HF	R3140	CTGCA/G
PstNI	CAGNNN/CTG	AlwNI	R0514	CAGNNN/CTG	AlwNI, CaiI
PsuGI x	BBCGD
PsuI	R/GATCY	BstYI	R0523	R/GATCY	BstX2I, BstYI, MflI
PsyI	GACN/NNGTC	PflFI	R0595	GACN/NNGTC	PflFI, Tth111I
		Tth111I	R0185	GACN/NNGTC
PteI	G/CGCGC	BssHII	R0199	G/CGCGC	BsePI, BssHII, PauI
PvuI-HF	CGAT/CG	PvuI-HF	R3150	CGAT/CG	Ple19I
PvuII	CAG/CTG	PvuII^§	R0151	CAG/CTG	PvuII-HF
		PvuII-HF	R3151	CAG/CTG
PvuII-HF	CAG/CTG	PvuII^§	R0151	CAG/CTG	PvuII
		PvuII-HF	R3151	CAG/CTG
Ran11014IV x	GAAAGAG
Rba2021I x	CACGAGH
RceI x	CATCGAC(20/18)
RdeGBI x	CCGCAG
RdeGBII x	ACCCAG(20/18)
RdeGBIII x	(9/11)TGRYCA(11/9)
Rer8036II x	CCGAKGG
RflFIII x	CGCCAG
RgaI	GCGAT/CGC	AsiSI	R0630	GCGAT/CGC	AsiSI, SfaAI, SgfI
Rgo13296IV x	GRAAGCG
Rho5650I x	AACGAG
RigI	GGCCGG/CC	FseI	R0588	GGCCGG/CC	FseI
Rkr11038I x	GGANNNNNRTGA
RlaI x	VCW
RlaII x	ACACAG(20/18)
RleAI x	CCCACA(12/9)
Rmu369III x	GGCYAC
RpaB5I x	CGRGGAC(20/18)
RpaBI x	CCCGCAG(20/18)
RpaI x	GTYGGAG(11/9)
RpaTI x	GRTGGAG
RruI	TCG/CGA	NruI-HF	R3192	TCG/CGA	Bsp68I, BtuMI, NruI-HF
RsaI	GT/AC	CviQI^	R0639	G/TAC	AfaI, Csp6I^, CviQI^, RsaNI^
		RsaI	R0167	GT/AC
RsaNI	G/TAC	CviQI	R0639	G/TAC	AfaI^, Csp6I, CviQI, RsaI^
		RsaI^	R0167	GT/AC
RseI	CAYNN/NNRTG	MslI	R0571	CAYNN/NNRTG	MslI, SmiMI
Rsp531II x	CACACG
Rsp008IV x	ACGCAG
RspPBTS2III x	CTTCGAG
Rsp008V x	GCCCAT
Rsr2I	CG/GWCCG	RsrII	R0501	CG/GWCCG	CpoI, CspI, RsrII
RsrII	CG/GWCCG	RsrII	R0501	CG/GWCCG	CpoI, CspI, Rsr2I
Rtr1953I x	TGANNNNNNTGA
SacI	GAGCT/C	Eco53kI^	R0116	GAG/CTC	Ecl136II^, EcoICRI^, Eco53kI^, Psp124BI, SacI-HF, SstI
		SacI^§	R0156	GAGCT/C
		SacI-HF	R3156	GAGCT/C
SacI-HF	GAGCT/C	Eco53kI^	R0116	GAG/CTC	Ecl136II^, EcoICRI^, Eco53kI^, Psp124BI, SacI, SstI
		SacI^§	R0156	GAGCT/C
		SacI-HF	R3156	GAGCT/C
SacII	CCGC/GG	SacII	R0157	CCGC/GG	Cfr42I, KspI, Sfr303I, SgrBI
Saf8902III x	CAATNAG
Sag901I x	GCAAAT
SalI	G/TCGAC	SalI ^§	R0138	G/TCGAC	SalI-HF
		SalI-HF	R3138	G/TCGAC
SalI-HF	G/TCGAC	SalI ^§	R0138	G/TCGAC	SalI
		SalI-HF	R3138	G/TCGAC
SanDI x	GG/GWCCC				KflI
SapI	GCTCTTC(1/4)	BspQI	R0712	GCTCTTC(1/4)	BspQI, LguI, PciSI
		SapI	R0569	GCTCTTC(1/4)
SaqAI	T/TAA	MseI	R0525	T/TAA	MseI, Tru1I, Tru9I
SatI	GC/NGC	Fnu4HI	R0178	GC/NGC	Fnu4HI, Fsp4HI
Sau3AI	/GATC	DpnII	R0543	/GATC	Bsp143I, BssMI, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, NdeII
		MboI	R0147	/GATC
		Sau3AI	R0169	/GATC
SauI x	CC/TNAGG	Bsu36I	R0524	CC/TNAGG	AxyI, Bse21I, Bsu36I, Eco81I
Sau96I	G/GNCC	Sau96I	R0165	G/GNCC	AspS9I, BmgT120I, Cfr13I, PspPI
Sau5656II x	GTTGCA
Sau1803III x	CGANNNNNNTAC
Sau64037IV x	GTANNNNNNTGG
SauMJ015III x	GARCNAG
Sba460II x	GGNGAYG
SbfI	CCTGCA/GG	SbfI^§	R0642	CCTGCA/GG	SbfI-HF, SdaI, Sse8387I
		SbfI-HF	R3642	CCTGCA/GG
SbfI-HF	CCTGCA/GG	SbfI^§	R0642	CCTGCA/GG	SbfI, SdaI, Sse8387I
		SbfI-HF	R3642	CCTGCA/GG
Sbo46I x	TGAAC
ScaI-HF	AGT/ACT	ScaI-HF	R3122	AGT/ACT	BmcAI, ZrmI
SchI	GAGTC(5/5)	MlyI	R0610	GAGTC(5/5)	MlyI, PleI^, PpsI^
		PleI^	R0515	GAGTC(4/5)
ScoDS2II x	GCTAAT
ScrFI	CC/NGG	ScrFI	R0110	CC/NGG	Bme1390I, BmrFI, BstSCI^, MspR9I, StyD4I^
		StyD4I^	R0638	/CCNGG
SdaI	CCTGCA/GG	SbfI^§	R0642	CCTGCA/GG	SbfI, SbfI-HF, Sse8387I
		SbfI-HF	R3642	CCTGCA/GG
SdeAI x	CAGRAG(21/19)
SdeOSI x	(11/13)GACNNNNRTGA(12/10)
SduI	GDGCH/C	Bsp1286I	R0120	GDGCH/C	Bsp1286I, MhlI
Sdy7136I x	GAGNNNNNTAA
Sdy9603I x	GCANNNNNNNTGA
Sdy5370I x	CACNNNNNTCY
SecI x	C/CNNGG	BsaJI	R0536	C/CNNGG	BsaJI, BseDI, BssECI
SenA1673III x	GNGGCAG
Sen17963III x	CCAAAC
Sen5794III x	ACGAACB
Sen6480IV x	GTTCAT
SenPU131IV x	GAANCAG
SenSARA26III x	ACRCAG
SenTFIV x	GATCAG
Sep11964I x	CGYCAT
Seq11824I x	CTANNNNNCTC
SetI	ASST/
SexAI	A/CCWGGT	SexAI	R0605	A/CCWGGT	CsiI, MabI
SfaAI	GCGAT/CGC	AsiSI	R0630	GCGAT/CGC	AsiSI, RgaI, SgfI
SfaNI	GCATC(5/9)	SfaNI	R0172	GCATC(5/9)	BmsI, LweI
SfcI	C/TRYAG	SfcI	R0561	C/TRYAG	BfmI, BstSFI
SfeI x	C/TRYAG	SfcI	R0561	C/TRYAG	BfmI, BstSFI, SfcI
SfiI	GGCCNNNN/NGGCC	SfiI	R0123	GGCCNNNN/NGGCC
Sfl13829III x	GNYCAG
SfoI	GGC/GCC	KasI^	R0544	G/GCGCC	DinI, EgeI, EheI, KasI^, Mly113I^^, NarI^^, PluTI^^^, SspDI^
		NarI^^	R0191	GG/CGCC
		PluTI^^^	R0713	GGCGC/C
		SfoI	R0606	GGC/GCC
Sfr274I	C/TCGAG	PaeR7I	R0177	C/TCGAG	PaeR7I, SlaI, XhoI
		XhoI	R0146	C/TCGAG
Sfr303I	CCGC/GG	SacII	R0157	CCGC/GG	Cfr42I, KspI, SacII, SgrBI
SfuI	TT/CGAA	BstBI	R0519	TT/CGAA	AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, NspV
SgeI	CNNGNNNNNNNNN/
SgfI	GCGAT/CGC	AsiSI	R0630	GCGAT/CGC	AsiSI, RgaI, SfaAI
SgrAI	CR/CCGGYG	SgrAI	R0603	CR/CCGGYG
SgrAII x	CGAGATC
SgrBI	CCGC/GG	SacII	R0157	CCGC/GG	Cfr42I, KspI, SacII, Sfr303I
SgrDI	CG/TCGACG
Sgr7807I x	GCCGAGG
SgrTI x	CCDS(10/14)
SgsI	GG/CGCGCC	AscI	R0558	GG/CGCGCC	AscI, PalAI
SimI x	GGGTC(-3/0)
SinI	G/GWCC	AvaII	R0153	G/GWCC	AvaII, Bme18I, Eco47I, VpaK11BI
SlaI	C/TCGAG	PaeR7I	R0177	C/TCGAG	PaeR7I, Sfr274I, XhoI
		XhoI	R0146	C/TCGAG
Sma325I x	ARCCCT
SmaI	CCC/GGG	SmaI	R0141	CCC/GGG	Cfr9I^, TspMI^, XmaI^
		TspMI^	R0709	C/CCGGG
		XmaI^	R0180	C/CCGGG
Sma10259II x	CAAAGA
SmaUMH5I x	CTTGAC
SmaUMH8I x	GCGAACB
SmiI	ATTT/AAAT	SwaI	R0604	ATTT/AAAT	SwaI
SmiMI	CAYNN/NNRTG	MslI	R0571	CAYNN/NNRTG	MslI, RseI
SmlI	C/TYRAG	SmlI	R0597	C/TYRAG	SmoI
SmoI	C/TYRAG	SmlI	R0597	C/TYRAG	SmlI
SnaBI	TAC/GTA	SnaBI	R0130	TAC/GTA	BstSNI, Eco105I
SnaI x	GTATAC	BstZ17I	R3594	GTA/TAC	BssNAI, Bst1107I, BstZ17I-HF
		BstZ17I-HF	R3594	GTA/TAC
Sna507VIII x	CRTTGAG
Sno506I x	GGCCGAG
SpeI-HF	A/CTAGT	SpeI-HF	R3133	A/CTAGT	AhlI, BcuI
Spe19205IV x	GGACY
SphI	GCATG/C	SphI^§	R0182	GCATG/C	PaeI, SphI-HF
		SphI-HF	R3182	GCATG/C
SphI-HF	GCATG/C	SphI^§	R0182	GCATG/C	PaeI, SphI
		SphI-HF	R3182	GCATG/C
SplI x	C/GTACG	BsiWI^§	R0553	C/GTACG	BsiWI, BsiWI-HF, Pfl23II, PspLI
		BsiWI-HF	R3553	C/GTACG
SpnRII x	TCGAG
SpoDI x	GCGGRAG
SrfI	GCCC/GGGC	SrfI	R0629	GCCC/GGGC
SseBI	AGG/CCT	StuI	R0187	AGG/CCT	Eco147I, PceI, StuI
Sse9I	/AATT	MluCI	R0538	/AATT	MluCI, TasI
Sse8387I	CCTGCA/GG	SbfI^§	R0642	CCTGCA/GG	SbfI, SbfI-HF, SdaI
		SbfI-HF	R3642	CCTGCA/GG
Sse232I x	CG/CCGGCG				MreI
Sse8647I x	AG/GWCCT
SsiI	CCGC(-3/-1)	AciI	R0551	CCGC(-3/-1)	AciI, BspACI
SspDI	G/GCGCC	KasI	R0544	G/GCGCC	DinI^, EgeI^, EheI^, KasI, Mly113I^^, NarI^^, PluTI^^^, SfoI^
		NarI^^	R0191	GG/CGCC
		PluTI^^^	R0713	GGCGC/C
		SfoI^	R0606	GGC/GCC
SspI-HF	AAT/ATT	SspI-HF	R3132	AAT/ATT
Ssp714II x	CGCAGCG
Ssp6803IV x	GAAGGC
SspJOR1II x	AGCGANC
SspMI	C/TAG	BfaI	R0568	C/TAG	BfaI, FspBI, MaeI, XspI
SstE37I x	CGAAGAC(20/18)
SstI	GAGCT/C	Eco53kI^	R0116	GAG/CTC	Ecl136II^, EcoICRI^, Eco53kI^, Psp124BI, SacI, SacI-HF
		SacI^§	R0156	GAGCT/C
		SacI-HF	R3156	GAGCT/C
Sth132I x	CCCG(4/8)
Sth20745III x	GGACGAC
SthSt3II x	GAAGT
StuI	AGG/CCT	StuI	R0187	AGG/CCT	Eco147I, PceI, SseBI
StyD4I	/CCNGG	ScrFI^	R0110	CC/NGG	Bme1390I^, BmrFI^, BstSCI, MspR9I^, ScrFI^
		StyD4I	R0638	/CCNGG
StyI-HF	C/CWWGG	StyI-HF	R3500	C/CWWGG	BssT1I, Eco130I, EcoT14I, ErhI
SurP32aII x	ACRGAG
SwaI	ATTT/AAAT	SwaI	R0604	ATTT/AAAT	SmiI
Sxy1780I x	GGGTNA
TaaI	ACN/GT	HpyCH4III	R0618	ACN/GT	Bst4CI, HpyCH4III
TagI x	AC/GT
TaiI	ACGT/	HpyCH4IV^	R0619	A/CGT	HpyCH4IV^, HpySE526I^, MaeII^
TaqII	GACCGA(11/9)
TaqIII x	CACCCA(11/9)
TaqI-v2	T/CGA	TaqI-v2	R0149	T/CGA
TasI	/AATT	MluCI	R0538	/AATT	MluCI, Sse9I
TatI	W/GTACW
TauI	GCSG/C
TfiI	G/AWTC	TfiI	R0546	G/AWTC	PfeI
TkoII x	TTCAAG(10/8)
TpyTP2I x	ACCAAG
Tru1I	T/TAA	MseI	R0525	T/TAA	MseI, SaqAI, Tru9I
Tru9I	T/TAA	MseI	R0525	T/TAA	MseI, SaqAI, Tru1I
TscAI	CASTGNN/	TspRI	R0582	CASTGNN/	TspRI
TseFI	/GTSAC	Tsp45I	R0583	/GTSAC	NmuCI, Tsp45I
TseI	G/CWGC	ApeKI	R0643	G/CWGC	ApeKI
		TseI	R0591	G/CWGC
TsoI x	TARCCA(11/9)
TspARh3I x	GRACGAC
Tsp4CI x	ACN/GT	HpyCH4III	R0618	ACN/GT	Bst4CI, HpyCH4III, TaaI
TspDTI	ATGAA(11/9)
TspEI x	/AATT	MluCI	R0538	/AATT	MluCI, Sse9I, TasI
TspGWI	ACGGA(11/9)
Tsp45I	/GTSAC	Tsp45I	R0583	/GTSAC	NmuCI, TseFI
TspMI	C/CCGGG	SmaI^	R0141	CCC/GGG	Cfr9I, SmaI^, XmaI
		TspMI	R0709	C/CCGGG
		XmaI	R0180	C/CCGGG
TspRI	CASTGNN/	TspRI	R0582	CASTGNN/	TscAI
TssI x	GAGNNNCTC
TstI x	(8/13)CACNNNNNNTCC(12/7)
TsuI x	GCGAC
Tth111I	GACN/NNGTC	PflFI	R0595	GACN/NNGTC	PflFI, PsyI
		Tth111I	R0185	GACN/NNGTC
Tth111II x	CAARCA(11/9)
UbaF14I x	CCANNNNNTCG
UbaF11I x	TCGTA
UbaF12I x	CTACNNNGTC
UbaF9I x	TACNNNNNRTGT
UbaF13I x	GAGNNNNNNCTGG
UbaPI x	CGAACG
Van91I	CCANNNN/NTGG	PflMI	R0509	CCANNNN/NTGG	AccB7I, PflMI
Van9116I x	CCKAAG
VchE4II x	RTAAAYG
Vdi96II x	GNCYTAG
Vha464I	C/TTAAG	AflII	R0520	C/TTAAG	AflII, BfrI, BspTI, BstAFI, MspCI
VneI	G/TGCAC	ApaLI	R0507	G/TGCAC	Alw44I, ApaLI
VpaK11BI	G/GWCC	AvaII	R0153	G/GWCC	AvaII, Bme18I, Eco47I, SinI
VpaSKIII x	CGTCAG
VspI	AT/TAAT	AseI	R0526	AT/TAAT	AseI, PshBI
Vtu19109I x	CACRAYC
WviI x	CACRAG(21/19)
XagI	CCTNN/NNNAGG	EcoNI	R0521	CCTNN/NNNAGG	BstENI, EcoNI
XapI	R/AATTY	ApoI-HF	R3566	R/AATTY	AcsI, ApoI-HF
XbaI	T/CTAGA	XbaI	R0145	T/CTAGA
Xca85IV x	TACGAG
XceI	RCATG/Y	NspI	R0602	RCATG/Y	BstNSI, NspI
XcmI	CCANNNNN/NNNNTGG	XcmI	R0533	CCANNNNN/NNNNTGG
XhoI	C/TCGAG	PaeR7I	R0177	C/TCGAG	PaeR7I, Sfr274I, SlaI
		XhoI	R0146	C/TCGAG
XhoII x	R/GATCY	BstYI	R0523	R/GATCY	BstX2I, BstYI, MflI, PsuI
XmaI	C/CCGGG	SmaI^	R0141	CCC/GGG	Cfr9I, SmaI^, TspMI
		TspMI	R0709	C/CCGGG
		XmaI	R0180	C/CCGGG
XmaIII x	C/GGCCG	EagI^§	R0505	C/GGCCG	BseX3I, BstZI, EagI-HFv2, EclXI, Eco52I
		EagI-HF	R3505	C/GGCCG
		EagI-HFv2	R3505	C/GGCCG
XmaJI	C/CTAGG	AvrII	R0174	C/CTAGG	AspA2I, AvrII, BlnI
XmiI	GT/MKAC	AccI	R0161	GT/MKAC	AccI, FblI
XmnI	GAANN/NNTTC	XmnI	R0194	GAANN/NNTTC	Asp700I, MroXI, PdmI
XspI	C/TAG	BfaI	R0568	C/TAG	BfaI, FspBI, MaeI, SspMI
Yps3606I x	CGGAAG
Yru12986I x	AGGAAG
ZraI	GAC/GTC	AatII^	R0117	GACGT/C	AatII^
		ZraI	R0659	GAC/GTC
ZrmI	AGT/ACT	ScaI	R3122	AGT/ACT	BmcAI, ScaI-HF
		ScaI-HF	R3122	AGT/ACT
Zsp2I	ATGCA/T	NsiI^§	R0127	ATGCA/T	EcoT22I, Mph1103I, NsiI, NsiI-HF
		NsiI-HF	R3127	ATGCA/T

§ An HF version of this enzyme is available.

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