Enzymes with Nonpalindromic Sequences
| Enzyme | Recognition Sequence |
|---|---|
| AatII | GACGT/C |
| Acc65I | G/GTACC |
| AccI | GT/MKAC |
| AclI | AA/CGTT |
| AfeI | AGC/GCT |
| AflII | C/TTAAG |
| AflIII | A/CRYGT |
| AgeI-HF® | A/CCGGT |
| AhdI | GACNNN/NNGTC |
| AleI-v2 | CACNN/NNGTG |
| AluI | AG/CT |
| AlwNI | CAGNNN/CTG |
| ApaI | GGGCC/C |
| ApaLI | G/TGCAC |
| ApeKI | G/CWGC |
| ApoI-HF | R/AATTY |
| AscI | GG/CGCGCC |
| AseI | AT/TAAT |
| AsiSI | GCGAT/CGC |
| AvaI | C/YCGRG |
| AvaII | G/GWCC |
| AvrII | C/CTAGG |
| BaeGI | GKGCM/C |
| BamHI § | G/GATCC |
| BamHI-HF® | G/GATCC |
| BanI | G/GYRCC |
| BanII | GRGCY/C |
| BclI § | T/GATCA |
| BclI-HF | T/GATCA |
| BfaI | C/TAG |
| BglI | GCCNNNN/NGGC |
| BglII | A/GATCT |
| BlpI | GC/TNAGC |
| BmtI-HF® | GCTAG/C |
| BsaAI | YAC/GTR |
| BsaBI | GATNN/NNATC |
| BsaHI | GR/CGYC |
| BsaJI | C/CNNGG |
| BsaWI | W/CCGGW |
| BsiEI | CGRY/CG |
| BsiHKAI | GWGCW/C |
| BsiWI § | C/GTACG |
| BsiWI-HF® | C/GTACG |
| BslI | CCNNNNN/NNGG |
| BsoBI | C/YCGRG |
| BspDI | AT/CGAT |
| BspEI | T/CCGGA |
| BspHI | T/CATGA |
| Bsp1286I | GDGCH/C |
| BsrFI-v2 | R/CCGGY |
| BsrGI-HF® | T/GTACA |
| BssHII | G/CGCGC |
| BstAPI | GCANNNN/NTGC |
| BstBI | TT/CGAA |
| BstEII-HF® | G/GTNACC |
| BstNI | CC/WGG |
| BstUI | CG/CG |
| BstXI | CCANNNNN/NTGG |
| BstYI | R/GATCY |
| BstZ17I-HF® | GTATAC |
| Bsu36I | CC/TNAGG |
| BtgI | C/CRYGG |
| Cac8I | GCN/NGC |
| ClaI | AT/CGAT |
| CviKI-1 | RG/CY |
| CviQI | G/TAC |
| DdeI | C/TNAG |
| DpnI | GA/TC |
| DpnII | /GATC |
| DraI | TTT/AAA |
| DraIII-HF® | CACNNN/GTG |
| DrdI | GACNNNN/NNGTC |
| EaeI | Y/GGCCR |
| EagI-HF® | C/GGCCG |
| Eco53kI | GAG/CTC |
| EcoNI | CCTNN/NNNAGG |
| EcoO109I | RG/GNCCY |
| EcoRI § | G/AATTC |
| EcoRI-HF® | G/AATTC |
| EcoRV § | GAT/ATC |
| EcoRV-HF® | GAT/ATC |
| FatI | /CATG |
| Fnu4HI | GC/NGC |
| FseI | GGCCGG/CC |
| FspI | TGC/GCA |
| HaeII | RGCGC/Y |
| HaeIII | GG/CC |
| HhaI | GCG/C |
| HincII | GTY/RAC |
| HindIII § | A/AGCTT |
| HindIII-HF® | A/AGCTT |
| HinfI | G/ANTC |
| HinP1I | G/CGC |
| HpaI | GTT/AAC |
| HpaII | C/CGG |
| HpyCH4III | ACN/GT |
| HpyCH4IV | A/CGT |
| HpyCH4V | TG/CA |
| Hpy99I | CGWCG/ |
| Hpy188I | TCN/GA |
| Hpy166II | GTN/NAC |
| Hpy188III | TC/NNGA |
| KasI | G/GCGCC |
| KpnI-HF® | GGTAC/C |
| MboI | /GATC |
| MfeI-HF® | C/AATTG |
| MluCI | /AATT |
| MluI-HF® | A/CGCGT |
| MscI | TGG/CCA |
| MseI | T/TAA |
| MslI | CAYNN/NNRTG |
| MspA1I | CMG/CKG |
| MspI | C/CGG |
| MwoI | GCNNNNN/NNGC |
| NaeI | GCC/GGC |
| NarI | GG/CGCC |
| NciI | CC/SGG |
| NcoI § | C/CATGG |
| NcoI-HF® | C/CATGG |
| NdeI | CA/TATG |
| NgoMIV | G/CCGGC |
| NheI-HF® | G/CTAGC |
| NlaIII | CATG/ |
| NlaIV | GGN/NCC |
| NotI § | GC/GGCCGC |
| NotI-HF® | GC/GGCCGC |
| NruI-HF® | TCG/CGA |
| NsiI § | ATGCA/T |
| NsiI-HF® | ATGCA/T |
| NspI | RCATG/Y |
| PacI | TTAAT/TAA |
| PaeR7I | C/TCGAG |
| PciI | A/CATGT |
| PflFI | GACN/NNGTC |
| PflMI | CCANNNN/NTGG |
| PluTI | GGCGC/C |
| PmeI | GTTT/AAAC |
| PmlI | CAC/GTG |
| PpuMI | RG/GWCCY |
| PshAI | GACNN/NNGTC |
| PsiI-v2 | TTA/TAA |
| PspGI | /CCWGG |
| PspOMI | G/GGCCC |
| PspXI | VC/TCGAGB |
| PstI § | CTGCA/G |
| PstI-HF® | CTGCA/G |
| PvuI-HF® | CGAT/CG |
| PvuII § | CAG/CTG |
| PvuII-HF® | CAG/CTG |
| RsaI | GT/AC |
| RsrII | CG/GWCCG |
| SacI-HF® | GAGCT/C |
| SacII | CCGC/GG |
| SalI § | G/TCGAC |
| SalI-HF® | G/TCGAC |
| Sau3AI | /GATC |
| Sau96I | G/GNCC |
| SbfI-HF® | CCTGCA/GG |
| ScaI-HF® | AGT/ACT |
| ScrFI | CC/NGG |
| SexAI | A/CCWGGT |
| SfcI | C/TRYAG |
| SfiI | GGCCNNNN/NGGCC |
| SfoI | GGC/GCC |
| SgrAI | CR/CCGGYG |
| SmaI | CCC/GGG |
| SmlI | C/TYRAG |
| SnaBI | TAC/GTA |
| SpeI-HF® | A/CTAGT |
| SphI § | GCATG/C |
| SphI-HF® | GCATG/C |
| SrfI | GCCC/GGGC |
| SspI-HF® | AAT/ATT |
| StuI | AGG/CCT |
| StyD4I | /CCNGG |
| StyI-HF® | C/CWWGG |
| SwaI | ATTT/AAAT |
| TaqI-v2 | T/CGA |
| TfiI | G/AWTC |
| TseI | G/CWGC |
| Tsp45I | /GTSAC |
| TspMI | C/CCGGG |
| TspRI | NNCASTGNN/ |
| Tth111I | GACN/NNGTC |
| XbaI | T/CTAGA |
| XcmI | CCANNNNN/NNNNTGG |
| XhoI | C/TCGAG |
| XmaI | C/CCGGG |
| XmnI | GAANN/NNTTC |
| ZraI | GAC/GTC |
§ A HF version of this enzyme is available.
